Book
Revealing and Characterizing Astrocytic Atrophy in an Animal Model of Depression
| Τίτλος: | Revealing and Characterizing Astrocytic Atrophy in an Animal Model of Depression |
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| Συγγραφείς: | Lin, Si-Si, Zhou, Bin, Tang, Yong, Verkhratsky, Alexei |
| Πηγή: | Methods in Molecular Biology ISBN: 9781071643655 Lin, S-S, Zhou, B, Tang, Y & Verkhratsky, A 2025, Revealing and Characterizing Astrocytic Atrophy in an Animal Model of Depression. in Methods in Molecular Biology. vol. 2896, Methods in molecular biology (Clifton, N.J.), Springer Nature, pp. 271-285. https://doi.org/10.1007/978-1-0716-4366-2_21 |
| Στοιχεία εκδότη: | Springer US, 2025. |
| Έτος έκδοσης: | 2025 |
| Θεματικοί όροι: | Male, Astrocytes/metabolism, Stress, Mice, Animals, Microscopy, Depressive Disorder, Behavior, Depressive Disorder, Major, Microscopy, Confocal/methods, Microscopy, Confocal, Cytoskeletal Proteins/metabolism, Behavior, Animal, Animal, Depression, Major/pathology, Disease Models, Animal, Confocal/methods, Stress, Psychological/metabolism, Astrocytes, Disease Models, Depression/pathology, Depressive Disorder, Major/pathology, Atrophy, Psychological/metabolism, Prefrontal Cortex/metabolism, Stress, Psychological |
| Περιγραφή: | Astrocyte atrophy is the main histopathological hallmark of major depressive disorder (MDD) in humans and in animal models of depression. In particular, depression and depressive-like behaviors are associated with a substantial decrease in size and complexity of astrocytes in the prefrontal cortex. This results in the reduced homeostatic support of synaptic transmission, which arguably translates into abnormal activity of neuronal networks and pathological changes in mood and behavior. Treatment of experimental animals with antidepressants as well as with acupuncture in the specific acupoints alleviates depressive-like behaviors and rescues astrocytic atrophy. Here, we describe the methodology for inducing and monitoring the depressive-like behaviors in mice using chronic unpredictable mild stress (CUMS), with subsequent in-depth analysis of astrocytic morphology using confocal microscopy in conjunction with astrocyte-specific labeling by virally transfected genetically encoded fluorescent probe mCherry and intracellular injection of Lucifer yellow. We also describe immunocytochemical visualization of astrocyte-specific cytoskeletal linker ezrin, which is involved in controlling perisynaptic astrocytic leaflets. |
| Τύπος εγγράφου: | Part of book or chapter of book Article |
| Γλώσσα: | English |
| DOI: | 10.1007/978-1-0716-4366-2_21 |
| Σύνδεσμος πρόσβασης: | https://pubmed.ncbi.nlm.nih.gov/40111613 https://research.manchester.ac.uk/en/publications/0dfed33e-b7e2-41ea-b1a9-3445b3a95a9b https://doi.org/10.1007/978-1-0716-4366-2_21 |
| Rights: | Springer Nature TDM |
| Αριθμός Καταχώρησης: | edsair.doi.dedup.....c122a0e114dcbf420834110c5ca1601f |
| Βάση Δεδομένων: | OpenAIRE |
| DOI: | 10.1007/978-1-0716-4366-2_21 |
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