Academic Journal
Development and Characterization of Synthetic Norovirus RNA for Use in Molecular Detection Methods
| Τίτλος: | Development and Characterization of Synthetic Norovirus RNA for Use in Molecular Detection Methods |
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| Συγγραφείς: | Eun-Jung Cho, Younggil Cha, Su Kyung Lee, Han-Sung Kim, Jae-Seok Kim, Eun Jin Lee, Nuri Lee, Ki Ho Hong, Hee Jin Huh, Young Joo Cha, Hyun Soo Kim |
| Συνεισφορές: | Eun-Jung Cho, Younggil Cha, Su Kyung Lee, Han-Sung Kim, Jae-Seok Kim, Eun Jin Lee, Nuri Lee, Ki Ho Hong, Hee Jin Huh, Young Joo Cha, Hyun Soo Kim, Hong, Ki Ho |
| Πηγή: | Ann Lab Med |
| Στοιχεία εκδότη: | Annals of Laboratory Medicine, 2022. |
| Έτος έκδοσης: | 2022 |
| Θεματικοί όροι: | 0301 basic medicine, 0303 health sciences, Genotype, Characterization, Noroviruses, Norovirus, Uncertainty, Viral / analysis, Real-Time Polymerase Chain Reaction, Multicenter study, 3. Good health, Real-Time Polymerase Chain Reaction / methods, 03 medical and health sciences, Caliciviridae Infections* / diagnosis, Viral / genetics, Republic of Korea, RNA, Humans, RNA, Viral, Original Article, Reference material, Norovirus* / genetics, Caliciviridae Infections |
| Περιγραφή: | Reference materials are essential for the quality assurance of molecular detection methods. We developed and characterized synthetic norovirus GI and GII RNA reference materials.Norovirus GI and GII RNA sequences including the ORF1-ORF2 junction region were designed based on 1,495 reported norovirus sequences and synthesized via plasmid preparation and in vitro transcription. The synthetic norovirus GI and GII RNAs were evaluated using six commercial norovirus detection kits used in Korea and subjected to homogeneity and stability analyses. A multicenter study involving five laboratories and using four commercial real-time PCR norovirus detection assays was conducted for synthetic norovirus RNA characterization and uncertainty measurements.The synthetic norovirus GI and GII RNAs were positively detected using the six commercial norovirus detection kits and were homogeneous and stable for one year when stored at -20°C or -70°C. All data from the five laboratories were within a range of 1.0 log copies/μL difference for each RNA, and the overall mean concentrations for norovirus GI and GII RNAs were 7.90 log copies/μL and 6.96 log copies/μL, respectively.The synthetic norovirus GI and GII RNAs are adequate for quality control based on commercial molecular detection reagents for noroviruses with high sequence variability. The synthetic RNAs can be used as reference materials in norovirus molecular detection methods. |
| Τύπος εγγράφου: | Article Other literature type |
| Γλώσσα: | English |
| ISSN: | 2234-3814 2234-3806 |
| DOI: | 10.3343/alm.2023.43.1.38 |
| Σύνδεσμος πρόσβασης: | https://pubmed.ncbi.nlm.nih.gov/36045055 |
| Rights: | CC BY NC CC BY NC ND URL: http://creativecommons.org/licenses/by-nc/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0 (http://creativecommons.org/licenses/by-nc/4.0/) ) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| Αριθμός Καταχώρησης: | edsair.doi.dedup.....bd459a2e20399bf0e966be9f3868d11f |
| Βάση Δεδομένων: | OpenAIRE |
| ISSN: | 22343814 22343806 |
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| DOI: | 10.3343/alm.2023.43.1.38 |