Academic Journal
A randomised controlled trial of amniotic membrane in the treatment of a standardised burn injury in the merino lamb
| Τίτλος: | A randomised controlled trial of amniotic membrane in the treatment of a standardised burn injury in the merino lamb |
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| Συγγραφείς: | Leila Cuttle, Margit Kempf, John F. Fraser, Gael E. Phillips, Roy M. Kimble, Mark Hayes |
| Συνεισφορές: | Wolf, S. |
| Πηγή: | Burns. 35:998-1003 |
| Στοιχεία εκδότη: | Elsevier BV, 2009. |
| Έτος έκδοσης: | 2009 |
| Θεματικοί όροι: | 0301 basic medicine, FIBROBLASTS, Ovine model, Amniotic membrane, 920117 Skin and Related Disorders, INTERLEUKIN (IL)-6, Cicatrix, Random Allocation, 03 medical and health sciences, C1, Scar, CORNEAL, PHORBOL ESTERS, IL-8 PRODUCTION, Animals, Skin/metabolism/pathology, GLUCOCORTICOIDS, Amnion, GROWTH-FACTORS, Fetal wound healing, Skin, Deep dermal burn injury, Wound Healing, 0303 health sciences, Sheep, Biological Dressings, CYTOKINES, EPITHELIAL-CELLS, Actins, 3. Good health, Amnion/transplantation, Disease Models, Animal, Cicatrix/metabolism/pathology/prevention and control, Actins/metabolism, Disease Models, 110304 Dermatology, Tissue and Organ Harvesting, Tissue and Organ Harvesting/methods, 110305 Emergency Medicine, Burns, SKIN |
| Περιγραφή: | Burn injury is associated with disabling scar formation which impacts on many aspects of the patient's life. Previously we have shown that the fetus heals a deep dermal burn in a scarless fashion. Amniotic membrane (AM) is the outermost fetal tisue and has beeen used as a dressing in thermal injuries, though there is little data to support this use. To assess the efficacy of AM in scar minimisation after deep dermal burn wound, we conducted a randomised controlled study in the 1-month lamb. Lambs were delivered by caesarian section and the amniotic membranes stored after which lambs were returned to their mothers post-operatively. At 1 month, a standardised deep dermal burn was created under general anaesthesia on both flanks of the lamb. One flank was covered with unmatched AM, the other with paraffin gauze. Animals were sequentially euthanased from Day 3-60 after injury and tissue analysed for histopathology and immunohistochemically for alpha-smooth muscle actin (alphaSMA) content. AM resulted in reduced scar tissue as assessed histopathologically and reduced alphaSMA content. This study provides the first laboratory evidence that AM may reduce scar formation after burn injury. |
| Τύπος εγγράφου: | Article |
| Γλώσσα: | English |
| ISSN: | 0305-4179 |
| DOI: | 10.1016/j.burns.2009.01.003 |
| Σύνδεσμος πρόσβασης: | https://pubmed.ncbi.nlm.nih.gov/19447551 http://www.sciencedirect.com/science/article/pii/S0305417909000266 https://www.sciencedirect.com/science/article/pii/S0305417909000266 https://core.ac.uk/display/19661259 https://espace.library.uq.edu.au/view/UQ:186183 https://www.ncbi.nlm.nih.gov/pubmed/19447551 |
| Rights: | Elsevier TDM |
| Αριθμός Καταχώρησης: | edsair.doi.dedup.....ba80c65b6b41e9a84f3c09ae2f1e853f |
| Βάση Δεδομένων: | OpenAIRE |
| ISSN: | 03054179 |
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| DOI: | 10.1016/j.burns.2009.01.003 |