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Identification of a DNA Cytosine Methyltransferase Gene in Conjugative Transposon Tn5252

Bibliographic Details
Title: Identification of a DNA Cytosine Methyltransferase Gene in Conjugative Transposon Tn5252
Authors: Janardhan Sampath, M. N. Vijayakumar
Source: Plasmid. 39:63-76
Publisher Information: Elsevier BV, 1998.
Publication Year: 1998
Subject Terms: DNA, Bacterial, 0301 basic medicine, Base Sequence, Sequence Homology, Amino Acid, Streptococcus pyogenes, Molecular Sequence Data, Gene Transfer Techniques, Gene Expression, Sequence Analysis, DNA, Open Reading Frames, 03 medical and health sciences, Streptococcus pneumoniae, Mutagenesis, Conjugation, Genetic, DNA Transposable Elements, Amino Acid Sequence, DNA (Cytosine-5-)-Methyltransferases
Description: The nucleotide sequence of the 3.5-kb right junction fragment of the streptococcal conjugative transposon Tn5252 was obtained. The DNA fragment was found to carry four putative genes one of which displayed a high degree of similarity to prokaryotic 5C-cytosine methyltransferases carrying multiple sequence specificities. No cognate endonuclease gene was detected in the sequenced DNA. Purified methylase polypeptide synthesized in a T7 promoter-controlled overexpression system was found to lack methylase activity while the cell extracts of host cells containing the recombinant plasmid carrying the methylase gene were active. In vivo mutations in the methylase gene did not seem to affect the transferability of the element.
Document Type: Article
Language: English
ISSN: 0147-619X
DOI: 10.1006/plas.1997.1316
Access URL: https://pubmed.ncbi.nlm.nih.gov/9473447
https://www.ncbi.nlm.nih.gov/pubmed/9473447
https://www.sciencedirect.com/science/article/abs/pii/S0147619X97913160
https://europepmc.org/abstract/MED/9473447
Rights: Elsevier TDM
Accession Number: edsair.doi.dedup.....ba2e657cbd34419bf2363b59d29af674
Database: OpenAIRE
Description
ISSN:0147619X
DOI:10.1006/plas.1997.1316