Academic Journal
Dynamics of cytoplasm and cleavage divisions correlates with preimplantation embryo development
| Title: | Dynamics of cytoplasm and cleavage divisions correlates with preimplantation embryo development |
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| Authors: | Szpila, Marcin, Ajduk, Anna, Milewski, Robert |
| Source: | Reproduction. 155:1-14 |
| Publisher Information: | Oxford University Press (OUP), 2018. |
| Publication Year: | 2018 |
| Subject Terms: | Male, 0301 basic medicine, Cytoplasm, Pregnancy Rate, Embryonic Development, Cytoplasm - metabolism, Fertilization in Vitro, intracytoplasmic, Sperm injections, Time-Lapse Imaging, Embryo Culture Techniques, Pregnancy rate, Mice, 03 medical and health sciences, Pregnancy, inbred C57BL, Animals, inbred CBA, Embryo Implantation, Sperm Injections, Intracytoplasmic, Embryonic development - physiology, Fertilization in vitro, Time-lapse imaging, Embryo culture techniques, Blastocyst - physiology, Embryo transfer, Blastocyst - cytology, Embryo Transfer, 16. Peace & justice, Mice, Inbred C57BL, Blastocyst, Embryo implantation, Mice, Inbred CBA, Female |
| Description: | In vitro fertilization has become increasingly popular as an infertility treatment. In order to improve efficiency of this procedure, there is a strong need for a refinement of existing embryo assessment methods and development of novel, robust and non-invasive selection protocols. Studies conducted on animal models can be extremely helpful here, as they allow for more extensive research on the potential biomarkers of embryo quality. In the present paper, we subjected mouse embryos to non-invasive time-lapse imaging and combined the Particle Image Velocimetry analysis of cytoplasmic dynamics in freshly fertilized oocytes with the morphokinetic analysis of recordings covering 5 days of preimplantation development. Our results indicate that parameters describing cytoplasmic dynamics and cleavage divisions independently correspond to mouse embryo’s capacity to form a high-quality blastocyst. We also showed for the first time that these parameters are associated with the percentage of abnormal embryonic cells with fragmented nuclei and with embryo’s ability to form primitive endoderm, one of the cell lineages differentiated during preimplantation development. Finally, we present a model that links selected cytoplasmic and morphokinetic parameters reflecting frequency of fertilization-induced Ca2+-oscillations and timing of 4-cell stage and compaction with viability of the embryo assessed as the total number of cells at the end of its preimplantation development. Our results indicate that a combined analysis of cytoplasmic dynamics and morphokinetics may facilitate the assessment of embryo’s ability to form high-quality blastocysts. |
| Document Type: | Article |
| Language: | English |
| ISSN: | 1741-7899 1470-1626 |
| DOI: | 10.1530/rep-17-0230 |
| Access URL: | https://rep.bioscientifica.com/downloadpdf/journals/rep/155/1/REP-17-0230.pdf https://pubmed.ncbi.nlm.nih.gov/28993454 https://repozytorium.uw.edu.pl//handle/item/105468 https://rep.bioscientifica.com/view/journals/rep/155/1/REP-17-0230.xml |
| Rights: | OUP Standard Publication Reuse |
| Accession Number: | edsair.doi.dedup.....ba248a9aec31cd07af9902bdf45a9b8f |
| Database: | OpenAIRE |
| ISSN: | 17417899 14701626 |
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| DOI: | 10.1530/rep-17-0230 |