Academic Journal
Cold-induced dishabituation in rodents exposed to recurrent hypoglycaemia
| Title: | Cold-induced dishabituation in rodents exposed to recurrent hypoglycaemia |
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| Authors: | Mark L. Evans, Jennifer E. Gallagher, Bernard Thorens, Bastiaan E. de Galan, Rory J. McCrimmon, Ulrik Pedersen-Bjergaard, Alison D. McNeilly, Keeran Vickneson, Jessica Blackburn |
| Contributors: | DSpace at Cambridge pro (8.1) |
| Source: | Diabetologia Diabetologia, 64, 6, pp. 1436-1441 Vickneson, K, Blackburn, J, Gallagher, J R, Evans, M L, de Galan, B E, Pedersen-Bjergaard, U, Thorens, B, McNeilly, A D & McCrimmon, R J 2021, ' Cold-induced dishabituation in rodents exposed to recurrent hypoglycaemia ', Diabetologia, vol. 64, no. 6, pp. 1436-1441 . https://doi.org/10.1007/s00125-021-05425-3 |
| Publisher Information: | Springer Science and Business Media LLC, 2021. |
| Publication Year: | 2021 |
| Subject Terms: | Blood Glucose, Male, 0301 basic medicine, Epinephrine, Short Communication, name=Internal Medicine, Rats, Sprague-Dawley, 03 medical and health sciences, 0302 clinical medicine, Animals, Insulin, Adaptation, Physiological, Counterregulatory responses, Hypoglycemia, Rats, 3. Good health, Diabetes and Metabolism, Cold Temperature, Type 1 diabetes, Impaired awareness, Glucose Clamp Technique, Habituation, Radboudumc 6: Metabolic Disorders RIMLS: Radboud Institute for Molecular Life Sciences, name=Endocrinology, Hypoglycaemia, Cold |
| Description: | Aims/hypothesis Recurrent hypoglycaemia in people with diabetes leads to progressive suppression of counterregulatory hormonal responses to subsequent hypoglycaemia. Recently it has been proposed that the mechanism underpinning this is a form of adaptive memory referred to as habituation. To test this hypothesis, we use two different durations of cold exposure to examine whether rodents exposed to recurrent hypoglycaemia exhibit two characteristic features of habituation, namely stimulus generalisation and dishabituation. Methods In the first study (stimulus generalisation study), hyperinsulinaemic–hypoglycaemic (2.8 mmol/l) glucose clamps were performed in non-diabetic rodents exposed to prior moderate-duration cold (4°C for 3 h) or control conditions. In the second study (dishabituation study), rodents exposed to prior recurrent hypoglycaemia or saline (154 mmol/l NaCl) injections over 4 weeks underwent a longer-duration cold (4°C for 4.5 h) exposure followed 24 h later by a hyperinsulinaemic–hypoglycaemic (2.8 mmol/l) glucose clamp. Output measures were counterregulatory hormone responses during experimental hypoglycaemia. Results Moderate-duration cold exposure blunted the adrenaline (epinephrine) response (15,266 ± 1920 vs 7981 ± 1258 pmol/l, Control vs Cold; p p Conclusions/interpretation Non-diabetic rodents exhibit two cardinal features of habituation, namely stimulus generalisation and dishabituation. These findings provide further support for the hypothesis that suppressed counterregulatory responses following exposure to recurrent hypoglycaemia in diabetes result from habituation. Graphical abstract |
| Document Type: | Article Other literature type |
| File Description: | application/pdf |
| Language: | English |
| ISSN: | 1432-0428 0012-186X |
| DOI: | 10.1007/s00125-021-05425-3 |
| DOI: | 10.17863/cam.63734 |
| Access URL: | https://link.springer.com/content/pdf/10.1007/s00125-021-05425-3.pdf https://pubmed.ncbi.nlm.nih.gov/33730186 https://hdl.handle.net/https://repository.ubn.ru.nl/handle/2066/235269 https://www.narcis.nl/publication/RecordID/oai%3Arepository.ubn.ru.nl%3A2066%2F235269 https://europepmc.org/article/MED/33730186 https://cris.maastrichtuniversity.nl/en/publications/cold-induced-dishabituation-in-rodents-exposed-to-recurrent-hypog https://link.springer.com/content/pdf/10.1007/s00125-021-05425-3.pdf https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8099849 https://link.springer.com/article/10.1007/s00125-021-05425-3 https://curis.ku.dk/ws/files/284192708/Vickneson2021_Article_Cold_inducedDishabituationInRo.pdf https://www.repository.cam.ac.uk/handle/1810/316622 https://doi.org/10.1007/s00125-021-05425-3 https://doi.org/10.17863/cam.63734 |
| Rights: | CC BY |
| Accession Number: | edsair.doi.dedup.....b70cdc622b28823d89f858461138c2ff |
| Database: | OpenAIRE |
| ISSN: | 14320428 0012186X |
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| DOI: | 10.1007/s00125-021-05425-3 |