Academic Journal
Local γ‐aminobutyric acid and glutamate circuit control of hypophyseotrophic corticotropin‐releasing factor neuron activity in the paraventricular nucleus of the hypothalamus
| Title: | Local γ‐aminobutyric acid and glutamate circuit control of hypophyseotrophic corticotropin‐releasing factor neuron activity in the paraventricular nucleus of the hypothalamus |
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| Authors: | Dominique Muller, Laszlo Vutskits, Peter Streit, Viktor Bartanusz, R. C. Gaillard, Jozsef Zoltan Kiss |
| Source: | European Journal of Neuroscience, Vol. 19, No 3 (2004) pp. 777-782 |
| Publisher Information: | Wiley, 2004. |
| Publication Year: | 2004 |
| Subject Terms: | 616.8, Corticotropin-Releasing Hormone, Corticotropin-Releasing Hormone/ metabolism, Glutamic Acid, Microscopy, Electron/methods, Bicuculline, Paraventricular Hypothalamic Nucleus/ cytology/drug effects, GABA Antagonists, Rats, Sprague-Dawley, 03 medical and health sciences, Organ Culture Techniques, 0302 clinical medicine, Immunohistochemistry/methods, Excitatory Amino Acid Agonists, Animals, Drug Interactions, Valine/ analogs & derivatives/pharmacology, Radiometry/methods, Radiometry, Evoked Potentials, 6-Cyano-7-nitroquinoxaline-2,3-dione, Neurons, Glutamic Acid/metabolism/ physiology, Synapses/drug effects/metabolism/ultrastructure, Excitatory Amino Acid Agonists/pharmacology, Immunohistochemistry, Electric Stimulation, ddc:616.8, Rats, Excitatory Amino Acid Antagonists/pharmacology, Microscopy, Electron, GABA Antagonists/pharmacology, Neurons/drug effects/metabolism/ physiology/ultrastructure, Gamma-Aminobutyric Acid/metabolism/ physiology, Evoked Potentials/drug effects, Synapses, Excitatory Amino Acid Antagonists, Bicuculline/pharmacology, Paraventricular Hypothalamic Nucleus |
| Description: | Paraventricular corticotropin‐releasing factor (CRF) neurons play a pivotal role in regulating neuroendocrine responses to stress. The mechanisms by which synaptic inputs control the activity of these neurons are not well understood. The present study was undertaken to determine the role of the intrinsic γ‐aminobutyric acid (GABA)‐ and glutamatergic neural circuits of the hypothalamic paraventricular nucleus (PVN) in the control of CRF neural activity. We show that in organotypic cultures of the PVN, blockade of the intrinsic GABAergic neurotransmission by the GABAA receptor antagonist bicuculline resulted in a significant increase in CRF secretion. The bicuculline‐induced CRF secretory activity was abolished by the coadministration of the selective α‐amino‐3‐hydroxy‐5‐methyl‐4‐isoxazoleprionic acid (AMPA)/kainate receptor antagonist 6‐cyano‐7‐nitroquinoxaline‐2,3‐dione (CNQX). Electrical stimulation of the CRF cell division elicited glutamatergic extracellular field potentials that were dramatically enhanced by bicuculline and were suppressed by CNQX. These results show that the functional activity of CRF neurons in organotypic cultures of the PVN is under a tonic inhibitory influence of an intrinsic GABAergic circuit. Suppression of GABAergic transmission appears to have a permissive role for inducing an increased secretory activity of CRF neurons that is driven by an excitatory glutamatergic network via AMPA/kainate receptors. |
| Document Type: | Article |
| File Description: | application/pdf |
| Language: | English |
| ISSN: | 1460-9568 0953-816X |
| DOI: | 10.1111/j.1460-9568.2004.03167.x |
| Access URL: | https://pubmed.ncbi.nlm.nih.gov/14984429 https://scholars.uthscsa.edu/en/publications/local-%CE%B3-aminobutyric-acid-and-glutamate-circuit-control-of-hypoph https://core.ac.uk/display/60785367 http://onlinelibrary.wiley.com/doi/10.1111/j.1460-9568.2004.03167.x/abstract https://www.ncbi.nlm.nih.gov/pubmed/14984429 https://archive-ouverte.unige.ch/unige:10176 https://archive-ouverte.unige.ch/unige:10176 |
| Rights: | Wiley Online Library User Agreement |
| Accession Number: | edsair.doi.dedup.....947a83086fa814dd52b9694e7e513435 |
| Database: | OpenAIRE |
| ISSN: | 14609568 0953816X |
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| DOI: | 10.1111/j.1460-9568.2004.03167.x |