Academic Journal
Rapid and reliable detection of bacterial endospores in environmental samples by diagnostic electron microscopy combined with X-ray microanalysis
| Τίτλος: | Rapid and reliable detection of bacterial endospores in environmental samples by diagnostic electron microscopy combined with X-ray microanalysis |
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| Συγγραφείς: | Laue, Michael, Fulda, Gerhard |
| Πηγή: | Journal of Microbiological Methods. 94:13-21 |
| Στοιχεία εκδότη: | Elsevier BV, 2013. |
| Έτος έκδοσης: | 2013 |
| Θεματικοί όροι: | 0301 basic medicine, Clostridium/ultrastructure, 610 Medizin, Bacillus, Microscopy Electron/methods, 03 medical and health sciences, Sulfur/analysis, Phosphorus/analysis, Environmental Microbiology, Bacillus/isolation & purification, Spores Bacterial/isolation & purification, Clostridium/chemistry, Electron Probe Microanalysis/methods, Clostridium, Spores, Bacterial, Bacillus/chemistry, ddc:610, Bacteriological Techniques, 0303 health sciences, Calcium/analysis, Bacillus/ultrastructure, Phosphorus, Clostridium/isolation & purification, Spores Bacterial/chemistry, 3. Good health, Microscopy, Electron, Bacteriological Techniques/methods, Calcium, Biological Markers/analysis, Spores Bacterial/ultrastructure, Biomarkers, Sulfur, Electron Probe Microanalysis |
| Περιγραφή: | Diagnostic negative staining electron microscopy is a front-line method for the rapid investigation of environmental and clinical samples in emergency situations caused by bioterrorism or outbreaks of an infectious disease. Spores of anthrax are one of the diagnostic targets in case of bioterrorism, because they have been used as a bio-weapon in the past and their production and transmission are rather simple. With negative staining electron microscopy bacterial spores can be identified based on their morphology at the single cell level. However, because of their particular density, no internal structures are visible which sometimes makes it difficult to distinguish spores from particles with a similar size and shape that are frequently present in environmental samples. Spores contain a high concentration of calcium ions besides other elements, which may allow a proper discrimination of spores from other suspicious particles. To investigate this hypothesis, negative staining electron microscopy, using either transmission or scanning electron microscopes, was combined with energy dispersive X-ray microanalysis, which reveals the element content of individual nanoparticles. A peak pattern consisting of calcium, sulphur and phosphorus was found as a typical signature within the X-ray spectrum of spores in various Clostridium and Bacillus species, including all strains of anthrax (Bacillus anthracis) tested. Moreover, spores could be reliably identified by this combined approach in environmental samples, like household products, soil or various presumed bioterrorist samples. In summary, the use of X-ray spectroscopy, either directly in the transmission electron microscope, or in a correlative approach by using scanning electron microscopy, improves the emergency diagnostics of suspicious environmental samples. |
| Τύπος εγγράφου: | Article |
| Περιγραφή αρχείου: | application/pdf |
| Γλώσσα: | English |
| ISSN: | 0167-7012 |
| DOI: | 10.1016/j.mimet.2013.03.026 |
| DOI: | 10.25646/1799 |
| Σύνδεσμος πρόσβασης: | https://edoc.rki.de/bitstream/176904/1874/1/210URfBebbuVo.pdf https://pubmed.ncbi.nlm.nih.gov/23603002 https://edoc.rki.de/bitstream/176904/1874/1/210URfBebbuVo.pdf https://www.ncbi.nlm.nih.gov/pubmed/23603002 https://pubmed.ncbi.nlm.nih.gov/23603002/ https://www.sciencedirect.com/science/article/pii/S0167701213001334 https://edoc.rki.de/handle/176904/1874 https://europepmc.org/article/MED/23603002 |
| Rights: | Elsevier TDM |
| Αριθμός Καταχώρησης: | edsair.doi.dedup.....8dad84f6ec4f5da294761f274db2cbd3 |
| Βάση Δεδομένων: | OpenAIRE |
| ISSN: | 01677012 |
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| DOI: | 10.1016/j.mimet.2013.03.026 |