Academic Journal
Unveiling Candida albicans intestinal carriage in healthy volunteers: the role of micro- and mycobiota, diet, host genetics and immune response
| Title: | Unveiling Candida albicans intestinal carriage in healthy volunteers: the role of micro- and mycobiota, diet, host genetics and immune response |
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| Authors: | Delavy, Margot, Sertour, Natacha, Patin, Etienne, Le Chatelier, Emmanuelle, Cole, Nathaniel, Dubois, Florian, Xie, Zixuan, Saint-André, Violaine, Manichanh, Chaysavanh, Walker, Alan, Quintana-Murci, Lluis, Duffy, Darragh, d'Enfert, Christophe, Bougnoux, Marie-Elisabeth, Consortium, Milieu Intérieur |
| Contributors: | Institut Català de la Salut, [Delavy M, Sertour N] Unité Biologie et Pathogénicité Fongiques, Institut Pasteur, Université Paris Cité INRAE, Paris, France. [Patin E] Human Evolutionary Genetics Unit, Institut Pasteur, Université Paris Cité, CNRS UMR2000, Paris, France. [Le Chatelier E] MGP MetaGénoPolis, INRA, Université Paris-Saclay, Jouy-en-Josas, France. [Cole N] The Rowett Institute, University of Aberdeen, Aberdeen, UK. [Dubois F] Translational Immunology Unit, Institut Pasteur, Université Paris Cité, Paris, France. Institut Pasteur, Université Paris Cité, CBUTechS, Paris, France. [Manichanh C] Grup de Recerca de Microbioma Intestinal, Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. Vall d’Hebron Hospital Universitari, Barcelona, Spain, Vall d'Hebron Barcelona Hospital Campus, Vougny, Marie-Christine, University of Aberdeen.Rowett Institute, Biologie et Pathogénicité fongiques - Fungal Biology and Pathogenicity (BPF), Institut Pasteur Paris (IP)-Université Paris Cité (UPCité)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Génétique Evolutive Humaine - Human Evolutionary Genetics, Institut Pasteur Paris (IP)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), MetaGenoPolis (MGP (US 1367)), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), University of Aberdeen, Immunologie Translationnelle - Translational Immunology lab, Institut Pasteur Paris (IP)-Université Paris Cité (UPCité), Cytometrie et Biomarqueurs – Cytometry and Biomarkers (UTechS CB), Vall d’Hebron Research Institute (VHIR), Hub Bioinformatique et Biostatistique - Bioinformatics and Biostatistics HUB, Hôpital Necker - Enfants Malades AP-HP, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), This work was supported by a grant from Agence Nationale de la Recherche (FunComPath ANR-14-IFEC-0004), the French Government’s Investissement d’Avenir program (Laboratoire d’Excellence Integrative Biology of Emerging Infectious Diseases ANR10-LABX-62-IBEID , and ANR-10-LABX-69-01 ), the European Union’s Horizon 2020 research and innovation program under the Marie Sklodowska-Curie action, Innovative Training Network (FunHoMic, Grant No. 812969), and the European Union’s Horizon 2020 Research and Innovation Program (HDM-FUN, Grant No. 847507). AWW and the Rowett Institute (University of Aberdeen) received core funding support from the Scottish Government’s Rural and Environmental Sciences and Analytical Services (RESAS)., ANR-14-IFEC-0004,FunComPath,From fungal commensalism to pathogenicity:dissection of the colonization-to-infection shift of Candida albicans(2014), ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), ANR-10-LABX-0069,MILIEU INTERIEUR,GENETIC & ENVIRONMENTAL CONTROL OF IMMUNE PHENOTYPE VARIANCE: ESTABLISHING A PATH TOWARDS PERSONALIZED MEDICINE(2010), European Project: 812969,H2020-MSCA-ITN-2018,FunHoMic(2019), European Project: 847507,H2020-SC1-2019-Two-Stage-RTD,HDM-FUN(2020) |
| Source: | Gut Microbes Scientia Scientia. Dipòsit d'Informació Digital del Departament de Salut instname Gut Microbes, Vol 15, Iss 2 (2023) |
| Publisher Information: | Informa UK Limited, 2023. |
| Publication Year: | 2023 |
| Subject Terms: | [SDV]Life Sciences [q-bio], FENÓMENOS Y PROCESOS::fenómenos microbiológicos::microbiota::microbiota intestinal, RC799-869, R Medicine, PSYCHIATRY AND PSYCHOLOGY::Behavior and Behavior Mechanisms::Psychology, Social::Life Style, MESH: Gastrointestinal Microbiome, MESH: Diet, colonization resistance, Candida albicans, microbiota, GWAS, Humans, Intestins - Microbiologia, European Commission, PSIQUIATRÍA Y PSICOLOGÍA::conducta y mecanismos de la conducta::psicología social::estilo de vida, MESH: Immunity, metagenomics, MESH: Humans, MESH: Candida albicans, Immunity, lifestyle factors, QR Microbiology, Diseases of the digestive system. Gastroenterology, host factors, ORGANISMOS::Eukaryota::hongos::Ascomycota::Saccharomycetales::Candida::Candida albicans, 3. Good health, QR, Gastrointestinal Microbiome, Diet, [SDV] Life Sciences [q-bio], PHENOMENA AND PROCESSES::Microbiological Phenomena::Microbiota::Gastrointestinal Microbiome, MESH: Genome-Wide Association Study, Dieta, mycobiota, ORGANISMS::Eukaryota::Fungi::Ascomycota::Saccharomycetales::Candida::Candida albicans, Research Paper, Genome-Wide Association Study |
| Description: | Candida albicans is a commensal yeast present in the gut of most healthy individuals but with highly variable concentrations. However, little is known about the host factors that influence colonization densities. We investigated how microbiota, host lifestyle factors, and genetics could shape C. albicans intestinal carriage in 695 healthy individuals from the Milieu Intérieur cohort. C. albicans intestinal carriage was detected in 82.9% of the subjects using quantitative PCR. Using linear mixed models and multiway-ANOVA, we explored C. albicans intestinal levels with regard to gut microbiota composition and lifestyle factors including diet. By analyzing shotgun metagenomics data and C. albicans qPCR data, we showed that Intestinimonas butyriciproducens was the only gut microbiota species whose relative abundance was negatively correlated with C. albicans concentration. Diet is also linked to C. albicans growth, with eating between meals and a low-sodium diet being associated with higher C. albicans levels. Furthermore, by Genome-Wide Association Study, we identified 26 single nucleotide polymorphisms suggestively associated with C. albicans colonization. In addition, we found that the intestinal levels of C. albicans might influence the host immune response, specifically in response to fungal challenge. We analyzed the transcriptional levels of 546 immune genes and the concentration of 13 cytokines after whole blood stimulation with C. albicans cells and showed positive associations between the extent of C. albicans intestinal levels and NLRP3 expression, as well as secreted IL-2 and CXCL5 concentrations. Taken together, these findings open the way for potential new interventional strategies to curb C. albicans intestinal overgrowth. |
| Document Type: | Article Other literature type Review |
| File Description: | application/pdf |
| Language: | English |
| ISSN: | 1949-0984 1949-0976 |
| DOI: | 10.1080/19490976.2023.2287618 |
| Access URL: | https://pubmed.ncbi.nlm.nih.gov/38017705 https://hdl.handle.net/11351/10849 https://doaj.org/article/ca1afc041cd943248806021151391300 https://pasteur.hal.science/pasteur-04323991v1/document https://doi.org/10.1080/19490976.2023.2287618 https://pasteur.hal.science/pasteur-04323991v1 |
| Rights: | CC BY URL: http://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (http://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The terms on which this article has been published allow the posting of the Accepted Manuscript in a repository by the author(s) or with their consent. |
| Accession Number: | edsair.doi.dedup.....89c88d57b994c0ffff3d9c317f33fd7d |
| Database: | OpenAIRE |
| ISSN: | 19490984 19490976 |
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| DOI: | 10.1080/19490976.2023.2287618 |