Academic Journal
ATR-FTIR Spectroscopy Highlights the Problem of Distinguishing Between Exophiala dermatitidis and E. phaeomuriformis Using MALDI-TOF MS
| Title: | ATR-FTIR Spectroscopy Highlights the Problem of Distinguishing Between Exophiala dermatitidis and E. phaeomuriformis Using MALDI-TOF MS |
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| Authors: | Macit Ilkit, Dilara Ogunc, Aylin Döğen, Ramazan Gümral, Yasemin Baygu, Yaşar Gök, Çağrı Ergin, Seyedmojtaba Seyedmousavi, Betil Ozhak-Baysan |
| Contributors: | Çukurova Üniversitesi |
| Source: | Microbial Ecology, 71, 2, pp. 339-46 |
| Publisher Information: | Springer Science and Business Media LLC, 2015. |
| Publication Year: | 2015 |
| Subject Terms: | 0301 basic medicine, Identification, chemistry, 03 medical and health sciences, Tandem Mass Spectrometry, tandem mass spectrometry, Spectroscopy, Fourier Transform Infrared, Exophiala, Matrix-Assisted Laser Desorption-Ionization, Humans, Chemical analysis, human, phaeohyphomycosis, procedures, infrared spectroscopy, Mycological Typing Techniques, Infrared spectroscopy, Spectroscopy, comparative study, Taxonomy, 0303 health sciences, Spectrometry, isolation and purification, microbiology, İdentification, Exophiala/*chemistry/classification/isolation & purification, Mycological Typing Techniques/*methods, Phaeohyphomycosis/*microbiology, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Spectroscopy, Fourier Transform Infrared/*methods, Tandem Mass Spectrometry/*methods, Mass, EMC MM-04-28-01, MALDI mass spectrometry, Phaeohyphomycosis, classification, Fourier Transform Infrared, İnfrared spectroscopy, matrix-assisted laser desorption-ionization mass spectrometry, microbiological examination, Radboudumc 4: lnfectious Diseases and Global Health RIHS: Radboud Institute for Health Sciences |
| Description: | The present study compared two chemical-based methods, namely, matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS) and attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy, to understand the misidentification of Exophiala dermatitidis and Exophiala phaeomuriformis. The study utilized 44 E. dermatitidis and 26 E. phaeomuriformis strains, which were partially treated with strong acids and bases for further evaluation. MALDI-TOF MS and ATR-FTIR spectroscopy data of the two Exophiala species were compared. Data groupings were observed for the chromic acid- and nitric acid-treated species when the black yeast sources were categorized as creosoted-oak sleepers, concrete sleepers, or dishwasher isolates. The MALDI-TOF MS data for the metalloenzyme-containing regions were consistent with the ATR-FTIR spectroscopy data. These results indicated that environmental isolates might contain metals not found in human isolates and might interfere with chemical-based identification methods. Therefore, MALDI-TOF MS reference libraries should be created for clinical strains and should exclude petroleum-associated environmental isolates. |
| Document Type: | Article |
| Language: | English |
| ISSN: | 1432-184X 0095-3628 |
| DOI: | 10.1007/s00248-015-0670-z |
| Access URL: | https://pubmed.ncbi.nlm.nih.gov/26373644 https://www.narcis.nl/publication/RecordID/oai%3Arepository.ubn.ru.nl%3A2066%2F172610 http://acikerisim.pau.edu.tr:8080/xmlui/handle/11499/9708?show=full https://dialnet.unirioja.es/servlet/articulo?codigo=5420146 https://link.springer.com/article/10.1007/s00248-015-0670-z https://repub.eur.nl/pub/84564 https://link.springer.com/content/pdf/10.1007%2Fs00248-015-0670-z.pdf http://hdl.handle.net/2066/172610 http://acikerisim.pau.edu.tr:8080/xmlui/handle/11499/9708 |
| Rights: | Springer TDM |
| Accession Number: | edsair.doi.dedup.....6ea164d83bc89e30a0e711cf892a1de4 |
| Database: | OpenAIRE |
| ISSN: | 1432184X 00953628 |
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| DOI: | 10.1007/s00248-015-0670-z |