Academic Journal
Carotenoid metabolic profiling and transcriptome‐genome mining reveal functional equivalence among blue‐pigmented copepods and appendicularia
| Title: | Carotenoid metabolic profiling and transcriptome‐genome mining reveal functional equivalence among blue‐pigmented copepods and appendicularia |
|---|---|
| Authors: | Nazia Mojib, Maan Amad, Manjula Thimma, Naroa Aldanondo, Mande Kumaran, Xabier Irigoien |
| Contributors: | Kumaran, Mande, KAUST, Analytical Core lab at KAUST, Bioscience Core lab at KAUST, Analytical Chemistry Core Lab, Biological and Environmental Sciences and Engineering (BESE) Division, Bioscience Core Lab, Engagement, Environmental, Marine Science Program, Plankton ecology Research Group, Red Sea Research Center (RSRC) |
| Source: | Molecular Ecology. 23:2740-2756 |
| Publisher Information: | Wiley, 2014. |
| Publication Year: | 2014 |
| Subject Terms: | 0301 basic medicine, mesozooplankton, β-carotene hydroxylase, Molecular Sequence Data, PROTEIN, Xanthophylls, SEQUENCE, 7. Clean energy, VERTICAL MIGRATION, ASTAXANTHIN, Mixed Function Oxygenases, 12. Responsible consumption, Copepoda, 03 medical and health sciences, carotenoid-binding proteins, Animals, high-resolution mass spectrometry, Amino Acid Sequence, Urochordata, 14. Life underwater, LOBSTER SHELL, MAXIMUM-LIKELIHOOD, Indian Ocean, Phylogeny, carotene hydroxylase, Pigmentation, BETA-CAROTENE, EVOLUTION, Lipocalins, astaxanthin, COLORATION MECHANISM, ZOOPLANKTON, 13. Climate action, Metabolome, Transcriptome, transcriptome |
| Description: | The tropical oligotrophic oceanic areas are characterized by high water transparency and annual solar radiation. Under these conditions, a large number of phylogenetically diverse mesozooplankton species living in the surface waters (neuston) are found to be blue pigmented. In the present study, we focused on understanding the metabolic and genetic basis of the observed blue phenotype functional equivalence between the blue‐pigmented organisms from the phylum Arthropoda, subclass Copepoda (Acartia fossae) and the phylum Chordata, class Appendicularia (Oikopleura dioica) in the Red Sea. Previous studies have shown that carotenoid–protein complexes are responsible for blue coloration in crustaceans. Therefore, we performed carotenoid metabolic profiling using both targeted and nontargeted (high‐resolution mass spectrometry) approaches in four different blue‐pigmented genera of copepods and one blue‐pigmented species of appendicularia. Astaxanthin was found to be the principal carotenoid in all the species. The pathway analysis showed that all the species can synthesize astaxanthin from β‐carotene, ingested from dietary sources, via 3‐hydroxyechinenone, canthaxanthin, zeaxanthin, adonirubin or adonixanthin. Further, using de novo assembled transcriptome of blue A. fossae (subclass Copepoda), we identified highly expressed homologous β‐carotene hydroxylase enzymes and putative carotenoid‐binding proteins responsible for astaxanthin formation and the blue phenotype. In blue O. dioica (class Appendicularia), corresponding putative genes were identified from the reference genome. Collectively, our data provide molecular evidences for the bioconversion and accumulation of blue astaxanthin–protein complexes underpinning the observed ecological functional equivalence and adaptive convergence among neustonic mesozooplankton. |
| Document Type: | Article |
| File Description: | application/pdf; application/vnd.openxmlformats-officedocument.spreadsheetml.sheet; application/octet-stream |
| Language: | English |
| ISSN: | 1365-294X 0962-1083 |
| DOI: | 10.1111/mec.12781 |
| DOI: | 10.5061/dryad.mm18b |
| Access URL: | https://pubmed.ncbi.nlm.nih.gov/24803335 https://onlinelibrary.wiley.com/doi/10.1111/mec.12781 https://dspace.azti.es/bitstream/24689/349/1/carotenoid%20metabolic.pdf https://repository.kaust.edu.sa/handle/10754/550807 https://onlinelibrary.wiley.com/doi/pdf/10.1111/mec.12781 https://repository.kaust.edu.sa/bitstream/10754/550807/1/Mojib_et_al-2014-Molecular_Ecology.pdf https://www.ncbi.nlm.nih.gov/pubmed/24803335 http://dspace.azti.es/handle/24689/349 |
| Rights: | CC BY NC ND |
| Accession Number: | edsair.doi.dedup.....5fb3faab279874a07a87eef2b2bc9f39 |
| Database: | OpenAIRE |
| ISSN: | 1365294X 09621083 |
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| DOI: | 10.1111/mec.12781 |