Academic Journal
Investigating the Interplay of SARS-CoV-2 RNAemia and Peripheral Inflammation in Platelet Dysfunction During Acute SARS-CoV-2 Infection
| Title: | Investigating the Interplay of SARS-CoV-2 RNAemia and Peripheral Inflammation in Platelet Dysfunction During Acute SARS-CoV-2 Infection |
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| Authors: | Mariangela Scavone, Roberta Rovito, Claudia Ghali, Antonella Fioretti, Bianca Clerici, Elena Bossi, Camilla Tincati, Andrea Santoro, Elisa Borghi, Gianmarco Podda, Giulia Carla Marchetti |
| Source: | Pathog Immun Pathogens and Immunity, Vol 10, Iss 2 (2025) |
| Publisher Information: | Case Western Reserve University, 2025. |
| Publication Year: | 2025 |
| Subject Terms: | Inflammation, Chemokines and Cytokines, SARS-CoV-2 viral RNA, Pathology, COVID-19, RB1-214, Immunologic diseases. Allergy, RC581-607, Platelets Dysfunction, Research Article |
| Description: | Background: Circulating degranulated platelets have been described during acute SARS-CoV-2 infection and associated with COVID-19 complications. This study investigated the relationship between the presence of plasma SARS-CoV-2 RNA (ie, SARS-CoV-2 RNAemia), systemic inflammation, and platelet dysfunction in a group of patients with COVID-19. Unlike our previous publication, which focused on platelet characterization, this work explores potential determinants of platelet activation, based on a distinct subset of patients with available stored samples. Methods: Patients with COVID-19 were stratified by platelet δ-granule content using the luciferin/luciferase assay into 2 groups: normal (COVδ-norm) and low (COVδ-low). Plasma SARS-CoV-2 RNAemia (RT-qPCR), cytokines, and chemokines (Cytometric Bead Array) were quantified on plasma samples. Markers of platelet activation were measured by flow cytometry in whole blood. Results: A total of 75 patients with COVID-19 were enrolled; 57 presented normal levels of platelet δ-granule content (COVδ-norm) and 18 had low levels of platelet δ-granules (COVδ-low). Groups were comparable in terms of age, sex, comorbidities, and SARS-CoV-2 RNAemia levels. Patients in the COVδ-low group showed significantly higher chemokine and cytokine levels compared to those in the COVδ-norm group, with strong correlations between IL-6, as well as granulocyte-macrophage colony-stimulating factor (GM-CSF), with platelet degranulation parameters. A similar trend, albeit less pronounced, was observed when patients were stratified based on their platelet activation phenotype. Conclusions: These findings suggest that peripheral inflammation, rather than SARS-CoV-2 RNAemia, is associated with platelet dysfunction during acute SARS-CoV-2 infection. |
| Document Type: | Article Other literature type |
| File Description: | application/pdf |
| ISSN: | 2469-2964 |
| DOI: | 10.20411/pai.v10i2.823 |
| Access URL: | https://doaj.org/article/d1938482fcc841aba9315290dfdff23c |
| Rights: | CC BY URL: http://creativecommons.org/licenses/by/4.0/This work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (http://creativecommons.org/licenses/by/4.0/) |
| Accession Number: | edsair.doi.dedup.....5d086facf1aac1c315a33fec50c08e0f |
| Database: | OpenAIRE |
| ISSN: | 24692964 |
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| DOI: | 10.20411/pai.v10i2.823 |