Academic Journal
Continuous centrifugal microfluidics (CCM) isolates heterogeneous circulating tumor cells via full automation
| Title: | Continuous centrifugal microfluidics (CCM) isolates heterogeneous circulating tumor cells via full automation |
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| Authors: | Woo, Hyeong Jung, Kim, Seung-Hoon, Kang, Hyo Jung, Lee, Soo-Hwan, Lee, Seung Joon, Kim, Jong Man, Gurel, Ogan, Kim, Soo Yeol, Roh, Hye Ran, Lee, Jungmin, Park, Yeonsoo, Shin, Hyun Young, Shin, Yong-Il, Lee, Sun Min, Oh, So Yeon, Kim, Young Zoon, Chae, Jung-Il, Lee, Seoyoung, Hong, Min Hee, Cho, Byoung Chul, Lee, Eun Sook, Pantel, Klaus, Kim, Hye Ryun, Kim, Minseok S. |
| Contributors: | Hyeong Jung Woo, Seung-Hoon Kim, Hyo Jung Kang, Soo-Hwan Lee, Seung Joon Lee, Jong Man Kim, Ogan Gurel, Soo Yeol Kim, Hye Ran Roh, Jungmin Lee, Yeonsoo Park, Hyun Young Shin, Yong-Il Shin, Sun Min Lee, So Yeon Oh, Young Zoon Kim, Jung-Il Chae, Seoyoung Lee, Min Hee Hong, Byoung Chul Cho, Eun Sook Lee, Klaus Pantel, Hye Ryun Kim, Minseok S Kim, Kim, Hye Ryun |
| Source: | Theranostics |
| Publisher Information: | Ivyspring International Publisher, 2022. |
| Publication Year: | 2022 |
| Subject Terms: | 0301 basic medicine, Microfluidics, ISOLATION PLATFORM, Cell Separation, cancer heterogeneity, circulating tumor cells, Neoplastic Cells, Cell Line, Automation, 03 medical and health sciences, Circulating* / metabolism, full automation, Cell Line, Tumor, Epithelial Cell Adhesion Molecule / genetics, Humans, 0303 health sciences, Tumor, DNA, Epithelial Cell Adhesion Molecule, Neoplastic Cells, Circulating, Cell Separation / methods, 3. Good health, ErbB Receptors, Microfluidics / methods, unbiased isolation, ErbB Receptors / genetics, continuous centrifugal microfluidics, Research Paper |
| Description: | Understanding cancer heterogeneity is essential to finding diverse genetic mutations in metastatic cancers. Thus, it is critical to isolate all types of CTCs to identify accurate cancer information from patients. Moreover, full automation robustly capturing the full spectrum of CTCs is an urgent need for CTC diagnosis to be routine clinical practice. Methods: Here we report the full capture of heterogeneous CTC populations using fully automated, negative depletion-based continuous centrifugal microfluidics (CCM). Results: The CCM system demonstrated high performance (recovery rates exceeding 90% and WBC depletion rate of 99.9%) across a wide range of phenotypes (EpCAM(+), EpCAM(-), small-, large-sized, and cluster) and cancers (lung, breast, and bladder). Applied in 30 lung adenocarcinoma patients harboring epidermal growth factor receptor (EGFR) mutations, the system isolated diverse phenotypes of CTCs in marker expression and size, implying the importance of unbiased isolation. Genetic analyses of intra-patient samples comparing cell-free DNA with CCM-isolated CTCs yielded perfect concordance, and CTC enumeration using our technique was correlated with clinical progression as well as response to EGFR inhibitors. Conclusion: Our system also introduces technical advances which assure rapid, reliable, and reproducible results, thus enabling a more comprehensive application of robust CTC analysis in clinical practice. |
| Document Type: | Article Other literature type |
| Language: | English |
| ISSN: | 1838-7640 |
| DOI: | 10.7150/thno.72511 |
| Access URL: | https://pubmed.ncbi.nlm.nih.gov/35664056 |
| Rights: | CC BY NC ND URL: http://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions. |
| Accession Number: | edsair.doi.dedup.....542406abaecc08d982fe76ffebc0e56a |
| Database: | OpenAIRE |
| ISSN: | 18387640 |
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| DOI: | 10.7150/thno.72511 |