Academic Journal
Concordance between two monoclonal antibody-based antigen detection enzyme-linked immunosorbent assays for measuring cysticercal antigen levels in sera from pigs experimentally infected with Taenia solium and Taenia hydatigena
| Τίτλος: | Concordance between two monoclonal antibody-based antigen detection enzyme-linked immunosorbent assays for measuring cysticercal antigen levels in sera from pigs experimentally infected with Taenia solium and Taenia hydatigena |
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| Συγγραφείς: | Gianfranco Arroyo, Luz Toribio, Sara Garrido, Nancy Chile, Teresa Lopez-Urbina, Luis A. Gomez-Puerta, Miguel Muro, Robert H. Gilman, Yesenia Castillo, Pierre Dorny, Seth E. O’Neal, Armando E. Gonzalez, Hector H. Garcia, for The Cysticercosis Working Group in Peru (CWGP) |
| Πηγή: | Parasit Vectors Parasites & Vectors, Vol 17, Iss 1, Pp 1-10 (2024) |
| Στοιχεία εκδότη: | Springer Science and Business Media LLC, 2024. |
| Έτος έκδοσης: | 2024 |
| Θεματικοί όροι: | Taenia hydatigena, 0301 basic medicine, Intelligent agents (Computer software) -- Applications to public health, Swine, Antibodies, Helminth, Taenia solium-- Treatment, Enzyme-Linked Immunosorbent Assay, Infectious and parasitic diseases, RC109-216, 03 medical and health sciences, 0302 clinical medicine, Concordance, Taenia solium, Medicine and Health Sciences, Animals, Humans, TsW8/TsW5, Antigens, Swine Diseases, Taenia, Cysticercosis, Cysts, Research, Antibodies, Monoclonal, Cysticercus, Ag-ELISA, 3. Good health, Antigens, Helminth, Monoclonal antibodies, Taenia solium -- Transmission |
| Περιγραφή: | Background Antigen detection in Taenia solium cysticercosis confirms viable infection in the intermediate host (either pig or human). The reference B158/B60 monoclonal antibody (mAb)-based Ag-enzyme-linked immunosorbent assay (ELISA) has acceptable levels of sensitivity and specificity in human neurocysticercosis with multiple brain cysts, although its sensitivity is lower in cases with single brain cysts, whereas in porcine cysticercosis the assay specificity is affected by its frequent cross-reaction with Taenia hydatigena, another common cestode found in pigs. Our group has produced 21 anti-T. solium mAbs reacting against antigens of the whole cyst, vesicular fluid, and secretory/excretory products, identifying TsW8/TsW5 as the most promising pair of mAbs for an Ag-ELISA. Methods We report the use of the TsW8/TsW5 Ag-ELISA to measure cysticercus antigen levels [expressed as optical density (OD) values] in two panels of sera collected from day 0 (baseline) to day 90 postinfection (PI) from pigs experimentally infected with T. solium (n = 26) and T. hydatigena (n = 12). At baseline and on days 28 and 90 PI, we used Bland–Altman (BA) analysis and Lin’s concordance correlation coefficients (CCC) to determine the concordance between the TsW8/TsW5 and the B158/B60 Ag-ELISA. Results The TsW8/TsW5 Ag-ELISA was able to efficiently measure circulating antigen levels in T. solium-infected pigs, similar to that obtained with the B158/B60 Ag-ELISA. Almost all paired log-OD differences between assays were within the limits of agreement (LoA) in the BA analysis at baseline and on days 28 and 90 PI (92.3%, 100%, and 100%, respectively), and a high concordance of log-ODs between assays was also found (Lin’s CCC: 0.69, 0.92, and 0.96, respectively, all P T. hydatigena, almost all paired log-OD differences were within the LoA in the BA analysis, whereas the concordance of log-ODs between assays was low at baseline (Lin’s CCC: 0.24) but increased on days 28 and 90 PI (Lins’ CCC: 0.88 and 0.98, P Conclusions/significance The TsW8/TsW5 Ag-ELISA recognizes antigens in pigs with T. solium cysticercosis and is highly concordant with the B158/B60 Ag-ELISA. However, its diagnostic use is hampered by cross-reactions with T. hydatigena, as in other mAb-based Ag-ELISAs. Graphical Abstract |
| Τύπος εγγράφου: | Article Other literature type |
| Περιγραφή αρχείου: | application/pdf |
| Γλώσσα: | English |
| ISSN: | 1756-3305 |
| DOI: | 10.1186/s13071-024-06197-6 |
| Σύνδεσμος πρόσβασης: | https://pubmed.ncbi.nlm.nih.gov/38566124 https://doaj.org/article/9d01066d0096446784fd9cc908b513ea |
| Rights: | CC BY URL: http://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (http://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (http://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
| Αριθμός Καταχώρησης: | edsair.doi.dedup.....427e8b3f850a6c3a6839104963dc5f51 |
| Βάση Δεδομένων: | OpenAIRE |
| ISSN: | 17563305 |
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| DOI: | 10.1186/s13071-024-06197-6 |