Academic Journal
Assessing spontaneous sensory neuron activity usingin vivocalcium imaging
| Title: | Assessing spontaneous sensory neuron activity usingin vivocalcium imaging |
|---|---|
| Authors: | Sonia L Ingram, Kim I Chisholm, Feng Wang, Yves De Koninck, Franziska Denk, George L Goodwin |
| Source: | Pain |
| Publisher Information: | Cold Spring Harbor Laboratory, 2023. |
| Publication Year: | 2023 |
| Subject Terms: | 0301 basic medicine, Action Potentials/physiology, Sensory Receptor Cells, Action Potentials, Pain, Lidocaine, Mice, 03 medical and health sciences, 0302 clinical medicine, Sensory Receptor Cells/physiology, Animals, Calcium, Research Paper |
| Description: | Heightened spontaneous activity in sensory neurons is often reported in individuals living with chronic pain. It is possible to study this activity in rodents using electrophysiology, but these experiments require great skill and can be prone to bias. Here, we have examined whetherin vivocalcium imaging with GCaMP6s can be used as an alternative approach. We show that spontaneously active calcium transients can be visualised in the fourth lumbar dorsal root ganglion (L4 DRG) viain vivoimaging in a mouse model of pain. Application of lidocaine to the nerve, between the inflamed site and the DRG, silenced spontaneous firing and revealed the true baseline level of calcium for spontaneously active neurons. We used this data to train a machine leaning algorithm to predict when a neuron is spontaneously active. We show that our algorithm is accurate in two different models of pain: intraplantar Complete Freund’s Adjuvant and antigen-induced arthritis, with accuracies of 90.0% +/-1.2 and 85.9 % +/-2.1, respectively, assessed against visual inspection by an experienced observer. The algorithm can also detect neuronal activity in imaging experiments generated in a different lab using a different microscope configuration (Accuracy = 94.0 % +/2.2). We provide a Google Colaboratory Notebook to allow anyone easy access to this novel tool, for assessment of peripheral neuron activity in their own calcium imaging setups. |
| Document Type: | Article Other literature type |
| ISSN: | 1872-6623 0304-3959 |
| DOI: | 10.1101/2023.01.12.523728 |
| DOI: | 10.1097/j.pain.0000000000003116 |
| Access URL: | https://pubmed.ncbi.nlm.nih.gov/38112748 |
| Rights: | CC BY |
| Accession Number: | edsair.doi.dedup.....233c8b4c869cfc5306a41f6781ce742a |
| Database: | OpenAIRE |
| ISSN: | 18726623 03043959 |
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| DOI: | 10.1101/2023.01.12.523728 |