Academic Journal
Ginsenoside Rg1 inhibits angiogenesis in diabetic retinopathy through the miR‐100‐3p/FBXW7/c‐MYC molecular axis
| Title: | Ginsenoside Rg1 inhibits angiogenesis in diabetic retinopathy through the miR‐100‐3p/FBXW7/c‐MYC molecular axis |
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| Authors: | Liping Xue, Min Hu, Yadi Li, Qin Zhu, Guanglong Zhou, Xiaofan Zhang, Yuan Zhou, Jieying Zhang, Peng Ding |
| Source: | J Diabetes Investig Journal of Diabetes Investigation, Vol 16, Iss 5, Pp 791-806 (2025) |
| Publisher Information: | Wiley, 2025. |
| Publication Year: | 2025 |
| Subject Terms: | Male, Diabetic Retinopathy, Ginsenoside Rg1, Ginsenosides, Neovascularization, Pathologic, Endothelial Cells, Articles, RC648-665, Diseases of the endocrine glands. Clinical endocrinology, Rats, Diabetes Mellitus, Experimental, Rats, Sprague-Dawley, Proto-Oncogene Proteins c-myc, MicroRNAs, Diabetic retinopathy, Animals, Humans, Angiogenesis |
| Description: | Aims/IntroductionGinsenoside Rg1 is an active ingredient found mainly in ginseng that has a variety of pharmacological effects, such as hypoglycemic, antioxidant, and anti‐inflammatory effects, and it inhibits vascular formation. In this study, we explored the effect of ginsenoside Rg1 on angiogenesis in diabetic retinopathy (DR) on the basis of its ability to inhibit angiogenesis and the specific molecular mechanism involved.Materials and MethodsWe induced an in vivo model of diabetes by injection of 55 mg/kg streptozotocin (STZ) into the abdominal cavity of SD rats daily for 3 days. Moreover, human retinal microvascular endothelial cells (HRMECs) were treated with 30 mmol/L glucose for 24 h to construct a high‐glucose (HG) cell model in vitro. The expression of related genes and proteins was detected by RT‐qPCR and Western blotting. HRMECs and retinal damage were evaluated by CCK‐8, scratch, tube formation assays, and HE staining.ResultsIn this study, Rg1 inhibited HG‐induced angiogenesis of HRMECs and inhibited STZ‐induced vascular leakage and capillary degeneration in vivo, alleviating the progression of DR. Mechanistically, Rg1 upregulated the expression of FBXW7 by inhibiting miR‐100‐3p, thereby promoting the ubiquitination and degradation of c‐MYC, inhibiting HG‐induced HRMECs proliferation, migration, invasion, and angiogenesis, and improving the development of DR.ConclusionsOverall, our study demonstrates that ginsenoside Rg1 can inhibit DR angiogenesis via the miR‐100‐3p/FBXW7/c‐MYC molecular axis. These findings provide a novel idea for the treatment of DR and provide an experimental basis for further research on the application of Rg1 in the treatment of DR. |
| Document Type: | Article Other literature type |
| Language: | English |
| ISSN: | 2040-1124 2040-1116 |
| DOI: | 10.1111/jdi.70016 |
| Access URL: | https://pubmed.ncbi.nlm.nih.gov/40033576 https://doaj.org/article/056e21ab49ca402fa96172d92fc82507 |
| Rights: | CC BY NC ND URL: http://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (http://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
| Accession Number: | edsair.doi.dedup.....0d934367064eae24ef918bb25bc57400 |
| Database: | OpenAIRE |
| ISSN: | 20401124 20401116 |
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| DOI: | 10.1111/jdi.70016 |