Academic Journal
Purification, characterization, and pathogenicity of urease produced by Vibrio parahaemolyticus.
| Τίτλος: | Purification, characterization, and pathogenicity of urease produced by Vibrio parahaemolyticus. |
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| Συγγραφείς: | Cai Y; Department of Clinical Laboratory, Shanghai No. 2 Medical University, P.R. China., Ni Y |
| Πηγή: | Journal of clinical laboratory analysis [J Clin Lab Anal] 1996; Vol. 10 (2), pp. 70-3. |
| Τύπος έκδοσης: | Journal Article; Research Support, Non-U.S. Gov't |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Wiley Country of Publication: United States NLM ID: 8801384 Publication Model: Print Cited Medium: Print ISSN: 0887-8013 (Print) Linking ISSN: 08878013 NLM ISO Abbreviation: J Clin Lab Anal Subsets: MEDLINE |
| Imprint Name(s): | Publication: Hoboken, N.J. : Wiley Original Publication: New York : Alan R. Liss, Inc., c1987- |
| Ιατρικοί όροι (MeSH): | Urease/*isolation & purification , Vibrio parahaemolyticus/*enzymology, Urease/chemistry ; Urease/metabolism ; Vibrio parahaemolyticus/chemistry ; Vibrio parahaemolyticus/pathogenicity ; Animals ; Animals, Suckling ; Electrophoresis, Polyacrylamide Gel ; Hot Temperature ; Hydrogen-Ion Concentration ; Isoelectric Focusing ; Mice ; Molecular Weight ; Rabbits |
| Περίληψη: | Vibrio parahaemolyticus is a halophilic bacterium associated with gastroenteritis and traveller's diarrhea. The urease-positive, Kanagawa-negative V. parahaemolyticus had been isolated from patients and the environment in the Pacific Northwest, first reported by Kelly et al. (5). Recently, we purified the urease produced by a clinical isolated of V. parahaemolyticus, and its characterization and pathogenicity has been studied. The urease isolation procedure included a water extraction and anion exchange chromatography. The molecular weight for the native enzyme was 275 KDa, and the three subunits of 85 KDa, 59 KDa, and 33 KDa were determined. The isoelectric focusing of urease was 5.2. The purified urease also can cause intestinal fluid accumulation and demonstrate a positive result in the suckling mouse test. These results suggested that the urease produced by V. parahaemolyticus may be another important indicator for the pathogenesis of the bacteria. |
| Substance Nomenclature: | EC 3.5.1.5 (Urease) |
| Entry Date(s): | Date Created: 19960101 Date Completed: 19961206 Latest Revision: 20081121 |
| Update Code: | 20260130 |
| DOI: | 10.1002/(SICI)1098-2825(1996)10:2<70::AID-JCLA2>3.0.CO;2-P |
| PMID: | 8852357 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 0887-8013 |
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| DOI: | 10.1002/(SICI)1098-2825(1996)10:2<70::AID-JCLA2>3.0.CO;2-P |