Academic Journal
Evaluation of a new urease reagent strip for detection of Helicobacter pylori in gastric biopsy specimens.
| Τίτλος: | Evaluation of a new urease reagent strip for detection of Helicobacter pylori in gastric biopsy specimens. |
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| Συγγραφείς: | Rogge JD; St. Francis Hospital Center, Beech Grove, Indiana, USA., Wagner DR, Carrico RJ, Glowinski EA, Mahoney SJ, Boguslaski RC, Genta RM |
| Πηγή: | The American journal of gastroenterology [Am J Gastroenterol] 1995 Nov; Vol. 90 (11), pp. 1965-8. |
| Τύπος έκδοσης: | Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Wolters Kluwer Health Country of Publication: United States NLM ID: 0421030 Publication Model: Print Cited Medium: Print ISSN: 0002-9270 (Print) Linking ISSN: 00029270 NLM ISO Abbreviation: Am J Gastroenterol Subsets: MEDLINE |
| Imprint Name(s): | Publication: <2019-> : [Philadelphia, PA] : Wolters Kluwer Health Original Publication: New York, Elsevier Science, -2003. |
| Ιατρικοί όροι (MeSH): | Reagent Strips*, Gastric Mucosa/*microbiology , Helicobacter Infections/*diagnosis , Helicobacter pylori/*isolation & purification , Urease/*analysis, Gastric Mucosa/pathology ; Helicobacter pylori/enzymology ; Pyloric Antrum/pathology ; Stomach/pathology ; Bacteriological Techniques ; Biopsy ; Evaluation Studies as Topic ; Humans ; Predictive Value of Tests ; Sensitivity and Specificity ; Specimen Handling ; Time Factors |
| Περίληψη: | Objectives: Determine sensitivity and specificity of a new urease reagent strip (URS) test for detection of Helicobacter pylori in gastric biopsy specimens. Methods: Six paired biopsy specimens were obtained from the greater curvature of the distal antrum, the lesser curvature near the incisura, and the corpus along the greater curvature during 66 procedures on 59 patients with endoscopic findings of gastric antral mucosal erythema or erosions, or gastric or duodenal ulcers. One biopsy from each site was tested with the URS. The second was evaluated with histology. A final antral biopsy was evaluated with a urea/gel test. Results: Twenty-eight of the 66 cases were histologically positive, with H. pylori observed in at least one of the three biopsy sites. The URS test correctly identified all 28. Of 193 individual biopsy specimens, 78 were positive for H. pylori. The URS correctly identified 77. Sensitivity was 0.99, specificity 0.95, positive predictive value 0.93, negative predictive value 0.99, and kappa 0.92. Average time to positive was 20 min. Twenty-seven antral biopsies were histologically positive for H. pylori. The URS test correctly identified all 27, whereas the urea/gel test correctly identified 21. For antral sites, URS sensitivity was 1.00 and specificity 0.95 versus urea/gel test sensitivity of 0.75 and specificity of 1.00. Conclusions: In this sample, the URS test is as accurate as histology in diagnosing H. pylori infections, and it provides results in less time and at a lower cost than histology. |
| Substance Nomenclature: | 0 (Reagent Strips) EC 3.5.1.5 (Urease) |
| Entry Date(s): | Date Created: 19951101 Date Completed: 19951208 Latest Revision: 20071115 |
| Update Code: | 20260130 |
| PMID: | 7485001 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 0002-9270 |
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