Academic Journal
Study of gamma radiation effects on the metabolic activity of Escherichia coli ATCC 25922 cells.
| Τίτλος: | Study of gamma radiation effects on the metabolic activity of Escherichia coli ATCC 25922 cells. |
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| Συγγραφείς: | Costa TS; Instituto Tecnológico de Aeronáutica , Departamento de Engenharia Eletrônica, Pça. Mal. do-Ar Eduardo Gomes, 50, Vila das Acacias, 12228-900 São José dos Campos, SP, Brazil., Mello DCR; Instituto Tecnológico de Aeronáutica , Departamento de Engenharia Eletrônica, Pça. Mal. do-Ar Eduardo Gomes, 50, Vila das Acacias, 12228-900 São José dos Campos, SP, Brazil., Federico CA; Instituto de Estudos Avançados, Divisão de Energia Nuclear, Trevo Coronel Aviador José Alberto Albano do Amarante, 1, Putim, 12228-001 São José dos Campos, SP, Brazil., Oliveira LD; Universidade Estadual de São Paulo Júlio de Mesquita Filho, Instituto de Ciência e Tecnologia, Departamento de Biociências e Diagnóstico Bucal, Av. Eng. Francisco José Longo, 777, Jardim São Dimas, 12245-000 São José dos Campos, SP, Brazil., Fernandes PC; Instituto Tecnológico de Aeronáutica , Departamento de Engenharia Eletrônica, Pça. Mal. do-Ar Eduardo Gomes, 50, Vila das Acacias, 12228-900 São José dos Campos, SP, Brazil. |
| Πηγή: | Anais da Academia Brasileira de Ciencias [An Acad Bras Cienc] 2026 Oct 12; Vol. 98 (4), pp. e20251237. Date of Electronic Publication: 2026 Oct 12 (Print Publication: 2026). |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Academia Brasileira De Ciencias Country of Publication: Brazil NLM ID: 7503280 Publication Model: eCollection Cited Medium: Internet ISSN: 1678-2690 (Electronic) Linking ISSN: 00013765 NLM ISO Abbreviation: An Acad Bras Cienc Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Rio De Janeiro : Academia Brasileira De Ciencias |
| Ιατρικοί όροι (MeSH): | Escherichia coli*/radiation effects , Escherichia coli*/metabolism , Biofilms*/radiation effects , Biofilms*/growth & development , Gamma Rays*, DNA Damage/radiation effects ; Oxidative Stress/radiation effects ; Dose-Response Relationship, Radiation |
| Περίληψη: | Investigating how Escherichia coli responds to gamma radiation enhances understanding of microbial functional mechanisms by elucidating how bacterial cells cope with ionizing radiation-induced DNA damage and oxidative stress. E. coli is a Gram-negative bacterium found in the intestinal microbiota of humans and widely used as a model organism in radiation microbiology. Exposure to ionizing radiation can impair cellular integrity, potentially leading to metabolic shifts and a loss of metabolic capacity. In this study, the effects of gamma radiation on E. coli populations were evaluated using MTT reduction assays as an operational proxy for metabolic activity rather than strict replicative viability. Irradiation experiments were performed using a fixed dose rate of 6 Gy/h with variable absorbed doses (0.05 to 40 Gy), as well as variable dose rates at a fixed dose of 6 Gy. Bacterial cells were suspended in BHI broth and incubated to allow biofilm formation. The results showed distinct metabolic profiles in planktonic and biofilm-associated cells at a constant dose rate, with dose-dependent alterations in metabolic output during biofilm formation. |
| Entry Date(s): | Date Created: 20261015 Date Completed: 20261015 Latest Revision: 20261015 |
| Update Code: | 20261016 |
| DOI: | 10.1590/0001-3765202620251237 |
| PMID: | 42879574 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1678-2690 |
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| DOI: | 10.1590/0001-3765202620251237 |