Academic Journal
Προβολή σε EDS
Συνδεδεμένο Πλήρες Κείμενο

Histological effects of chitosan, chitosan-carried hyaluronic acid, and photobiomodulation on palatal wound healing in rats: an in vivo study.

Λεπτομέρειες βιβλιογραφικής εγγραφής
Τίτλος: Histological effects of chitosan, chitosan-carried hyaluronic acid, and photobiomodulation on palatal wound healing in rats: an in vivo study.
Συγγραφείς: Esfahanizadeh N; Department of Periodontics, Faculty of Dentistry, Tehran Medical Sciences, Islamic Azad University, 19395-1495, Tehran, Iran., Soleimani M; Department of Periodontics, Faculty of Dentistry, Tehran Medical Sciences, Islamic Azad University, 19395-1495, Tehran, Iran., Ghaemi S; Department of Periodontics, Faculty of Dentistry, Tehran Medical Sciences, Islamic Azad University, 19395-1495, Tehran, Iran., Mortazavi P; Department of Pathology, Faculty of Specialized Veterinary Sciences, Science and Research Branch, Islamic Azad University, 1477893855, Tehran, Iran., Hoseinzadeh HA; Department of Clinical Sciences, Karaj Branch, Islamic Azad University, 3149968111, Karaj, Iran., Hakimiha N; Laser Application in Medical Sciences Research Center, Shahid Beheshti University of Medical Sciences, 1989934148, Tehran, Iran., Hatami H; Department of Pharmaceutics, School of Pharmacy, Mashhad University of Medical Sciences, 91775-1365, Mashhad, Iran., Mosaddad SA; Department of Research Analytics , Saveetha Institute of Medical and Technical Sciences,Saveetha Dental College and Hospitals, Saveetha University, 600077, Tamil Nadu, Chennai, India. semosadd@ucm.es.; Department of Conservative Dentistry and Prosthodontics, Faculty of Dentistry, Complutense University of Madrid (UCM), 28040, Plaza Ramón y Cajal s/n, Madrid, Spain. semosadd@ucm.es.
Πηγή: Clinical oral investigations [Clin Oral Investig] 2026 Oct 15; Vol. 30 (11). Date of Electronic Publication: 2026 Oct 15.
Τύπος έκδοσης: Journal Article
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: Springer-Verlag Country of Publication: Germany NLM ID: 9707115 Publication Model: Electronic Cited Medium: Internet ISSN: 1436-3771 (Electronic) Linking ISSN: 14326981 NLM ISO Abbreviation: Clin Oral Investig Subsets: MEDLINE
Imprint Name(s): Publication: Berlin : Springer-Verlag
Original Publication: Berlin : Springer, c1997-
Ιατρικοί όροι (MeSH): Hyaluronic Acid*/pharmacology , Chitosan*/pharmacology , Wound Healing*/drug effects , Wound Healing*/radiation effects , Palate*/injuries , Palate*/pathology , Low-Level Light Therapy*, Animals ; Rats, Wistar ; Male ; Rats ; Re-Epithelialization ; Random Allocation
Περίληψη: Objectives: To compare the histological effects of topical chitosan (CS), a hyaluronic acid-chitosan gel (HA/CS), and photobiomodulation (PBM) on palatal wound healing in rats.
Methods: Sixty male Wistar rats were randomized to control, CS, HA/CS, or PBM groups (n = 15/group). Standardized 5-mm full-thickness palatal wounds were created. Gels were applied three times daily; PBM (660 nm, 150 mW, 4.2 J/cm², 14 s) was administered on days 0, 1, 3, 5, 7, 10, and 13. Five independent animals/group were assessed on days 3, 7, and 14. The animal was the experimental unit, and re-epithelialization was the prespecified primary outcome. Ordinal scores were compared at each endpoint using Kruskal-Wallis and Bonferroni-adjusted Dunn tests.
Results: HA/CS had higher re-epithelialization scores than control on days 3 (adjusted p = 0.003) and 7 (adjusted p = 0.024), and than PBM on day 3 (adjusted p = 0.015). PBM exceeded control on day 7 (adjusted p = 0.024). All day-14 animals had the maximum epithelial score. HA/CS had higher fibroblast scores than control on day 3; all interventions had higher fibroblast and angiogenesis scores than control on days 7 and 14. Collagen scores exceeded control with CS and HA/CS on day 3 and HA/CS on days 7 and 14.
Conclusions: The tested HA/CS formulation showed favorable early epithelial and selected stromal findings. Day-14 epithelial scores showed a ceiling effect. HA-specific effects and HA-CS synergy cannot be established.
(© 2026. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Competing Interests: Declarations. Ethics approval and consent to participate: The animal study protocol was approved by the Ethics Committee of the Faculty of Dentistry,Tehran Medical Sciences, Islamic Azad University (IR.IAU.DENTAL.REC.1402.116). Consent for publication: Not applicable. Competing interests: The authors declare no competing interests.
References: Tavelli L, Barootchi S, Stefanini M, Zucchelli G, Giannobile WV, Wang HL (2023) Wound healing dynamics, morbidity, and complications of palatal soft-tissue harvesting. Periodontol 2000 92: 90–119 https://doi.org/10.1111/prd.12466.
Politis C, Schoenaers J, Jacobs R, Agbaje JO (2016) Wound healing problems in the mouth. Front Physiol 7:507. https://doi.org/10.3389/fphys.2016.00507. (PMID: 10.3389/fphys.2016.00507278534355089986)
Oliveira JA, da Silveira MI, Soares LFF, Alves RDO, Carrera TMI, Azevedo MR, Oliveira GJPL, Pigossi SC (2024) Wound-healing agents for palatal donor area: A network meta-analysis. Clin Oral Implants Res 35:359–376. https://doi.org/10.1111/clr.14241. (PMID: 10.1111/clr.1424138315151)
de Almeida MCL, Rocha RGG, Magno MB, Lima RR, Saito MT (2024) Performance of multiple therapeutic approaches for palatal wound healing after soft tissue graft removal: An overview of systematic reviews. Clin Oral Investig 28:347. https://doi.org/10.1007/s00784-024-05733-z. (PMID: 10.1007/s00784-024-05733-z38819478)
Tong W, Ren J, Yao X, Wang S, Li W, Zheng L, Hong X, Zhao S (2025) Biomaterials strategy for promoting palatal wound healing. Front Bioeng Biotechnol 13:1646629. https://doi.org/10.3389/fbioe.2025.1646629. (PMID: 10.3389/fbioe.2025.16466294096443612437701)
Aranaz I, Alcántara AR, Civera MC, Arias C, Elorza B, Heras Caballero A (2021) Acosta, N. Chitosan: An overview of its properties and applications. Polymers 13:3256. https://doi.org/10.3390/polym13193256. (PMID: 10.3390/polym13193256346410718512059)
Matica MA, Aachmann FL, Tøndervik A, Sletta H, Ostafe V (2019) Chitosan as a wound dressing starting material: Antimicrobial properties and mode of action. Int J Mol Sci 20:5889. https://doi.org/10.3390/ijms20235889. (PMID: 10.3390/ijms20235889317712456928789)
Yadav VS, Makker K, Haidrus R, Dawar A, Gumber B (2024) Chitosan-based dressing for management of palatal donor site: A randomized clinical trial. J Periodontal Res 59:1153–1161. https://doi.org/10.1111/jre.13267. (PMID: 10.1111/jre.1326738594813)
Iacob AT, Drăgan M, Ghețu N, Pieptu D, Vasile C, Buron F, Routier S, Giusca SE, Caruntu ID, Profire L (2018) Preparation, characterization and wound healing effects of new membranes based on chitosan, hyaluronic acid and arginine derivatives. Polymers 10:607. https://doi.org/10.3390/polym10060607. (PMID: 10.3390/polym10060607309666416404145)
Shabani M, Sharifi S, Karimi I, Shirian S, Fadaei M, Mirzaei E (2024) Evaluation of the restorative effect of ozone and chitosan–hyaluronic acid with and without mesenchymal stem cells on wound healing in rats. Vet Med Sci 10:e1439. https://doi.org/10.1002/vms3.1439. (PMID: 10.1002/vms3.14393869520811063917)
Joshi VM, Kandaswamy E, St. Germain J, Schiavo JH, Shaikh FMH (2024) Effect of hyaluronic acid on palatal wound healing: A systematic review. Clin Oral Investig 28:565. https://doi.org/10.1007/s00784-024-05955-1. (PMID: 10.1007/s00784-024-05955-139358570)
Yıldırım S, Özener HÖ, Doğan B, Kuru B (2018) Effect of topically applied hyaluronic acid on pain and palatal epithelial wound healing: An examiner-masked, randomized, controlled clinical trial. J Periodontol 89:36–45. https://doi.org/10.1902/jop.2017.170105. (PMID: 10.1902/jop.2017.17010528914592)
Lee JH, Lee KE, Nam OH, Chae YK, Lee MH, Kweon DK, Kim MS, Lee HS, Choi SC (2022) Orodispersible hyaluronic acid film delivery for oral wound healing in rats. J Dent Sci 17:1595–1603. https://doi.org/10.1016/j.jds.2022.04.004. (PMID: 10.1016/j.jds.2022.04.004362993359588893)
Bulut T, Ercan N (2025) The effect of polyvinylpyrrolidone–sodium hyaluronate gel on palatal wound healing: A randomized controlled clinical trial. BMC Oral Health 25:1305. https://doi.org/10.1186/s12903-025-06677-w. (PMID: 10.1186/s12903-025-06677-w4078161612333267)
Dahi M, Amash S, Bertl K, Beck F, Heimel P, Stavropoulos A (2026) Effect of hyaluronic acid on palatal wound healing—a randomized crossover clinical trial. Clin Oral Implants Res advance online publication. https://doi.org/10.1111/clr.70159.
Dompe C, Moncrieff L, Matys J, Grzech-Leśniak K, Kocherova I, Bryja A, Bruska M, Dominiak M, Mozdziak P, Skiba THI et al (2020) Photobiomodulation—Underlying mechanism and clinical applications. J Clin Med 9:1724. https://doi.org/10.3390/jcm9061724. (PMID: 10.3390/jcm9061724325032387356229)
Otterço AN, Andrade AL, Brassolatti P, Pinto KNZ, Araújo HSS, Parizotto NA (2018) Photobiomodulation mechanisms in the kinetics of the wound healing process in rats. J Photochem Photobiol B 183:22–29. https://doi.org/10.1016/j.jphotobiol.2018.04.010. (PMID: 10.1016/j.jphotobiol.2018.04.01029684717)
Morshedzadeh G, Aslroosta H, Vafaei M (2022) Effect of GaAlAs 940 nm photobiomodulation on palatal wound healing after free gingival graft surgery: A split-mouth randomized controlled clinical trial. BMC Oral Health 22:202. https://doi.org/10.1186/s12903-022-02229-8. (PMID: 10.1186/s12903-022-02229-8356107029131644)
Gopal L, Palwankar P, Dhalla N (2024) The efficacy of low-level laser therapy on the healing of oral wounds: a systematic review. Cureus ,16, e70832. https://doi.org/10.7759/cureus.70832.
Chaushu L, Atzil S, Vered M, Chaushu G, Matalon S, Weinberg E (2021) Age-related palatal wound healing: An experimental in vivo study. Biology 10:240. https://doi.org/10.3390/biology10030240. (PMID: 10.3390/biology10030240338088828003724)
Liu Y, Zhang S, Sakran KA, Yin J, Lan M, Yang C, Wang Y, Zeng N, Huang H, Shi B (2022) Observation of palatal wound healing process following various degrees of mucoperiosteal and bone trauma in a young rat model. Biology 11:1142. https://doi.org/10.3390/biology11081142. (PMID: 10.3390/biology11081142360097699405411)
Keswani SG, Balaji S, Le LD, Leung A, Parvadia JK, Frischer J et al (2013) Role of salivary VEGF in palatal mucosal wound healing. Wound Repair Regen 21:554–562. https://doi.org/10.1111/wrr.12065. (PMID: 10.1111/wrr.12065237582124528184)
Percie du Sert N, Hurst V, Ahluwalia A, Alam S, Avey MT, Baker M et al (2020) The ARRIVE guidelines 2.0: Updated guidelines for reporting animal research. PLoS Biol 18:e3000410. https://doi.org/10.1371/journal.pbio.3000410. (PMID: 10.1371/journal.pbio.3000410326632197360023)
Lee JH, Lee KE, Kang SW, Park SH, Chae YK, Lee MH et al (2024) Effect of orodispersible hyaluronic acid film on palatal mucosa wound healing. Oral Dis 30:518–527. https://doi.org/10.1111/odi.14517. (PMID: 10.1111/odi.1451736691707)
Ikinci G, Şenel S, Akıncıbay H, Kaş S, Erciş S, Wilson CG, Hıncal AA (2002) Effect of chitosan on a periodontal pathogen Porphyromonas gingivalis. Int J Pharm 235:121–127. https://doi.org/10.1016/S0378-5173(01)00974-7. (PMID: 10.1016/S0378-5173(01)00974-711879747)
Gal P, Kilik R, Mokry M, Vidinsky B, Vasilenko T, Mozes S, Bobrov N, Tomori Z, Bober J, Lenhardt L (2008) Simple method of open skin wound healing model in corticosteroid-treated and diabetic rats: Standardization of semi-quantitative and quantitative histological assessments. Vet Med 53:652–659. https://doi.org/10.17221/1973-VETMED. (PMID: 10.17221/1973-VETMED)
Nabiyi P, Jenabian N, Kazemi S, Moghadamnia AA, Seyedmajidi SA (2024) Effect of hyaluronic acid mucoadhesives on palatal wound healing and postoperative discomfort in free gingival graft surgery: A clinical trial. Front Dent 21:42. https://doi.org/10.18502/fid.v21i42.16848. (PMID: 10.18502/fid.v21i42.168483990637111792737)
Mauriello L, Pezzella V, Blasi A, Lanzillo SG, Iorio-Siciliano V, Ramaglia L (2026) Effects of hyaluronic acid as an adjunct to nonsurgical treatment of periodontal pockets: A narrative review. Quintessence Int 57:6–14. https://doi.org/10.3290/j.qi.b6689073. (PMID: 10.3290/j.qi.b668907341283209)
Iorio-Siciliano V, Blasi A, Mauriello L, Salvi GE, Ramaglia L, Sculean A (2025) Non-surgical treatment of moderate periodontal intrabony defects with adjunctive cross-linked hyaluronic acid: A single-blinded randomized controlled clinical trial. J Clin Periodontol 52:310–322. https://doi.org/10.1111/jcpe.14078. (PMID: 10.1111/jcpe.1407839402910)
Iorio-Siciliano V, Blasi A, Mauriello L, Windisch P, Salvi GE, Sculean A, Ramaglia L (2026) Non-surgical submarginal instrumentation of peri-implant mucositis with delivery of sodium hypochlorite/amino acids and cross-linked hyaluronic acid: A randomized clinical trial. Clin Oral Implants Res 37:643–656. https://doi.org/10.1111/clr.70109. (PMID: 10.1111/clr.701094176144213155298)
Yakout BK, El-Kimary GI, Heikal LAH, Khadr M, Kamel FR (2023) Efficacy of hyaluronic acid gel and photobiomodulation therapy on wound healing after surgical gingivectomy: A randomized controlled clinical trial. BMC Oral Health 23:805. https://doi.org/10.1186/s12903-023-03519-5. (PMID: 10.1186/s12903-023-03519-53789154910612174)
de Castro JR, da Silva Pereira F, Chen L, Arana-Chavez VE, Ballester RY, DiPietro LA et al (2020) Improvement of full-thickness rat skin wounds by photobiomodulation therapy: A dosimetric study. J Photochem Photobiol B 206:111850. https://doi.org/10.1016/j.jphotobiol.2020.111850. (PMID: 10.1016/j.jphotobiol.2020.11185032203726)
Mauriello L, Cuozzo A, Pezzella V, Iorio-Siciliano V, Isola G, Spagnuolo G, Ramaglia L, Blasi A (2025) Effects of photobiomodulation therapy (PBMT) in the management of postoperative pain after third lower molar extraction: A narrative review. J Clin Med 14:5210. https://doi.org/10.3390/jcm14155210. (PMID: 10.3390/jcm141552104080683212347340)
Contributed Indexing: Keywords: Chitosan; Hyaluronic acid; Low-level light therapy; Palate, hard; Rats, wistar; Wound healing
Substance Nomenclature: 9004-61-9 (Hyaluronic Acid)
9012-76-4 (Chitosan)
Entry Date(s): Date Created: 20261015 Date Completed: 20261015 Latest Revision: 20261015
Update Code: 20261016
DOI: 10.1007/s00784-026-07221-y
PMID: 42878922
Βάση Δεδομένων: MEDLINE
Περιγραφή
ISSN:1436-3771
DOI:10.1007/s00784-026-07221-y