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A multi-omic dissection of spatial hierarchy-dependent grain development in barley (Hordeum vulgare L.).

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Τίτλος: A multi-omic dissection of spatial hierarchy-dependent grain development in barley (Hordeum vulgare L.).
Συγγραφείς: Hong Y; Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops/Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, Yangzhou, 225009, China., Wu H; Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops/Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, Yangzhou, 225009, China., Su X; Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops/Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, Yangzhou, 225009, China., Zhu J; Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops/Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, Yangzhou, 225009, China., Lv C; Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops/Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, Yangzhou, 225009, China., Guo B; Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops/Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, Yangzhou, 225009, China., Wang F; Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops/Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, Yangzhou, 225009, China., Zhaxi L; Xizang Academy of Agriculture and Animal Husbandry Sciences, Research Institute of Agriculture, Lhasa, 850002, Xizang, China. zhaxiluobu2025@126.com., Xu R; Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops/Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, Yangzhou, 225009, China. rgxu@yzu.edu.cn.
Πηγή: TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik [Theor Appl Genet] 2026 Oct 14; Vol. 139 (11). Date of Electronic Publication: 2026 Oct 14.
Τύπος έκδοσης: Journal Article
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: Springer Country of Publication: Germany NLM ID: 0145600 Publication Model: Electronic Cited Medium: Internet ISSN: 1432-2242 (Electronic) Linking ISSN: 00405752 NLM ISO Abbreviation: Theor Appl Genet Subsets: MEDLINE
Imprint Name(s): Original Publication: Berlin, New York, Springer
Ιατρικοί όροι (MeSH): Hordeum*/genetics , Hordeum*/growth & development , Edible Grain*/genetics , Edible Grain*/growth & development , Seeds*/growth & development , Seeds*/genetics , Quantitative Trait Loci*, Chromosomes, Plant/genetics ; Chromosome Mapping ; Genome-Wide Association Study ; Phenotype ; Gene Expression Regulation, Plant ; Transcriptome ; Multiomics ; Polymorphism, Single Nucleotide
Περίληψη: Key Message: GWAS on grain uniformity and transcriptomics across spike shapes/positions reveal checkpoints in barley grainheterogeneity development. Grain uniformity, defined as the consistency of grain size among siblings within a sample, can influence yield performance and malting quality in barley, yet its genetic basis remains poorly understood. Here, we combine genome-wide association analysis of 334 barley accessions with spatiotemporal transcriptomics of two cultivars exhibiting contrasting uniformity. We identified six quantitative trait loci (QTLs) across chromosomes 1H, 2H, 4H, and 5H. The non-uniform cultivar displayed 3,314 differentially expressed genes between apical and basal grains at grain filling stage, versus only 222 in the uniform cultivar. This approximately 15-fold difference was accompanied by positional differences in starch biosynthesis, sugar transport, and trehalose-6-phosphate signaling. We identified four candidate genes proximal to stable GWAS signals on chromosome 1H and developed a dCAPS marker. These findings identify grain filling stage as a key stage associated with grain uniformity and suggest asymmetric carbon metabolism as a candidate mechanism underlying spatial hierarchy-dependent grain development.
(© 2026. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Competing Interests: Declarations. Conflicts of interest: The authors declare no conflict of interest. Ethical approval: Not applicable. Consent to participate: Not applicable. Consent for publication: Not applicable.
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Grant Information: XZ202502ZY0003 Science and Technology Projects of Xizang Autonomous Region, China; CARS-05 the National Modern Agriculture Industry Technology System, China
Entry Date(s): Date Created: 20261014 Date Completed: 20261014 Latest Revision: 20261014
Update Code: 20261015
DOI: 10.1007/s00122-026-05414-3
PMID: 42873280
Βάση Δεδομένων: MEDLINE
Περιγραφή
ISSN:1432-2242
DOI:10.1007/s00122-026-05414-3