Academic Journal
Bioorthogonal Chemistry in Neurobiology: From Imaging to Proteomics.
| Τίτλος: | Bioorthogonal Chemistry in Neurobiology: From Imaging to Proteomics. |
|---|---|
| Συγγραφείς: | Taylor N; Werner Reichardt Centre for Integrative Neuroscience, University of Tübingen, Tübingen, Germany., de Sousa-Lourenço J; Luxembourg Centre for Systems Biomedicine (LCSB), University of Luxembourg, Esch-sur-Alzette, Luxembourg., Nikić-Spiegel I; Werner Reichardt Centre for Integrative Neuroscience, University of Tübingen, Tübingen, Germany.; Luxembourg Centre for Systems Biomedicine (LCSB), University of Luxembourg, Esch-sur-Alzette, Luxembourg. |
| Πηγή: | Chembiochem : a European journal of chemical biology [Chembiochem] 2026 Oct 28; Vol. 27 (20), pp. e202500949. |
| Τύπος έκδοσης: | Journal Article; Review |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Wiley-VCH Verlag Country of Publication: Germany NLM ID: 100937360 Publication Model: Print Cited Medium: Internet ISSN: 1439-7633 (Electronic) Linking ISSN: 14394227 NLM ISO Abbreviation: Chembiochem Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Weinheim, Germany : Wiley-VCH Verlag, c2000- |
| Ιατρικοί όροι (MeSH): | Click Chemistry*/methods , Proteomics*/methods , Amino Acids*/chemistry , Proteins*/chemistry , Proteins*/metabolism , Neurobiology*/methods, Humans ; Animals |
| Περίληψη: | Bioorthogonal click chemistry has emerged as a versatile approach for probing molecules with high selectivity and minimal perturbation. While applicable to diverse biomolecules, its use in protein studies has proven particularly powerful, especially in neurobiology, where precise molecular tools are essential. A key technology enabling such precision is genetic code expansion (GCE), which allows the incorporation of unnatural amino acids (UAAs) bearing bioorthogonal handles into proteins. These UAAs can be introduced site-specifically to label defined proteins or proteome-wide for systems-level analysis. Complementing this, proteome-wide labeling can be achieved through metabolic incorporation of amino acid analogs. Coupled with copper-catalyzed or strain-promoted click reactions, these labeling technologies enable applications ranging from protein visualization to mass spectrometry-based proteomics. Ongoing advances in UAA incorporation strategies and the chemical diversity of available UAAs continue to broaden the opportunities to study protein expression, localization, and dynamics. In this review, we highlight recent methodological developments in click chemistry-based protein labeling and their applications in neurobiology. (© 2026 The Author(s). ChemBioChem published by Wiley‐VCH GmbH.) |
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| Grant Information: | 317530061 Deutsche Forschungsgemeinschaft; 101068378 HORIZON EUROPE Marie Sklodowska-Curie Actions |
| Contributed Indexing: | Keywords: click chemistry; fluorescence microscopy; neural proteomics; protein labeling; unnatural amino acids |
| Substance Nomenclature: | 0 (Amino Acids) 0 (Proteins) |
| Entry Date(s): | Date Created: 20261014 Date Completed: 20261014 Latest Revision: 20261016 |
| Update Code: | 20261016 |
| PubMed Central ID: | PMC13662511 |
| DOI: | 10.1002/cbic.202500949 |
| PMID: | 42870666 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1439-7633 |
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| DOI: | 10.1002/cbic.202500949 |