Academic Journal
Fine mapping of QPm.caas-4BL.2 for adult-plant resistance to powdery mildew in wheat.
| Title: | Fine mapping of QPm.caas-4BL.2 for adult-plant resistance to powdery mildew in wheat. |
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| Authors: | Dong Y; Crop Research Institute, Ningxia Academy of Agriculture and Forestry Sciences, 490 Huanghe Road, Yinchuan, Ningxia, 750002, China.; Institute of Crop Sciences, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, Beijing, 100081, China., Dong Y; Institute of Crop Sciences, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, Beijing, 100081, China., Xu X; Institute of Crop Sciences, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, Beijing, 100081, China., Ren Y; College of Agronomy, Henan Agricultural University, 218 Pingan Road, Zhengzhou, Henan, 450046, China., Liu J; Institute of Crop Sciences, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, Beijing, 100081, China., Xu D; College of Agronomy, Qingdao Agricultural University, 700 Changcheng Road, Qingdao, Shandong, 266109, China., Hao Y; Institute of Crop Sciences, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, Beijing, 100081, China., Yang Y; College of Agronomy, Henan Agricultural University, 218 Pingan Road, Zhengzhou, Henan, 450046, China., Zhang W; Crop Research Institute, Ningxia Academy of Agriculture and Forestry Sciences, 490 Huanghe Road, Yinchuan, Ningxia, 750002, China., Kang L; Crop Research Institute, Ningxia Academy of Agriculture and Forestry Sciences, 490 Huanghe Road, Yinchuan, Ningxia, 750002, China., He J; Crop Research Institute, Ningxia Academy of Agriculture and Forestry Sciences, 490 Huanghe Road, Yinchuan, Ningxia, 750002, China., Cao L; Crop Research Institute, Ningxia Academy of Agriculture and Forestry Sciences, 490 Huanghe Road, Yinchuan, Ningxia, 750002, China., Ren N; Crop Research Institute, Ningxia Academy of Agriculture and Forestry Sciences, 490 Huanghe Road, Yinchuan, Ningxia, 750002, China., Zhang X; Institute of Crop Sciences, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, Beijing, 100081, China., He Z; Institute of Crop Sciences, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, Beijing, 100081, China.; International Maize and Wheat Improvement Center (CIMMYT) China Office, c/o CAAS, 12 Zhongguancun South Street, Beijing, 100081, China., Xia X; Institute of Crop Sciences, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, Beijing, 100081, China. xiaxianchun@caas.cn., Chen D; Crop Research Institute, Ningxia Academy of Agriculture and Forestry Sciences, 490 Huanghe Road, Yinchuan, Ningxia, 750002, China. cdsnky@163.com. |
| Source: | TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik [Theor Appl Genet] 2026 Oct 13; Vol. 139 (11). Date of Electronic Publication: 2026 Oct 13. |
| Publication Type: | Journal Article |
| Language: | English |
| Journal Info: | Publisher: Springer Country of Publication: Germany NLM ID: 0145600 Publication Model: Electronic Cited Medium: Internet ISSN: 1432-2242 (Electronic) Linking ISSN: 00405752 NLM ISO Abbreviation: Theor Appl Genet Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Berlin, New York, Springer |
| MeSH Terms: | Triticum*/genetics , Triticum*/microbiology , Plant Diseases*/microbiology , Plant Diseases*/genetics , Disease Resistance*/genetics , Quantitative Trait Loci* , Ascomycota*, Chromosomes, Plant/genetics ; Genetic Markers ; Chromosome Mapping ; Haplotypes ; Phenotype ; Polymorphism, Single Nucleotide ; Physical Chromosome Mapping ; Genes, Plant ; Genotype |
| Abstract: | Key Message: We fine-mapped QPm.caas-4BL.2 for adult-plant resistance to powdery mildew in an 880-kb physical interval, predicted a candidate gene, identified a favorable haplotype in wheat, and developed a CAPS marker for marker-assisted breeding. Powdery mildew, a globally devastating disease, severely impairs both grain yield and quality in wheat. Identification of powdery mildew resistance genes is essential for marker-assisted selection of disease-resistant wheat cultivars. In the present study, we conducted fine mapping of the QTL QPm.caas-4BL.2 for adult-plant resistance to powdery mildew from the Zhou8425B/Chinese Spring recombinant inbred line (RIL) population. By integrating genotypic and phenotypic data from progeny populations of 63 heterozygous recombinant individuals, QPm.caas-4BL.2 was mapped to an 880-kb physical interval (611.71-612.59 Mb) on chromosome 4B, containing 12 high-confidence annotated genes in the Chinese Spring reference genome (IWGSC RefSeq v2.1). TraesCS4B03G0836900 (annotated as an MLO) was considered a potential causal gene of QPm.caas-4BL.2 based on gene sequence polymorphisms, transcriptome profiles, and haplotype analysis. The TraesCS4B03G0836900 gene contains seven SNPs (single nucleotide polymorphisms) and formed six haplotypes in 145 re-sequenced wheat accessions, with Hap5-mlo carrying a missense mutation that showed significantly lower disease severity than other haplotypes. A cleaved amplified polymorphic sequence (CAPS) marker targeting the missense mutation was developed and validated in the Zhou8425B-derived varieties. Varieties carrying Zhou8425B allele exhibited 6.9% reduction in disease severity compared to those with Chinese Spring allele. This study provides genes, molecular markers, and resistant germplasm for improvement of powdery mildew resistance in wheat cultivars. (© 2026. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.) |
| Competing Interests: | Declarations. Ethics approval and consent to participate: These experiments complied with the ethical standards in China. Competing interests: The authors declare no competing interests. |
| References: | Theor Appl Genet. 2012 Jul;125(2):297-309. (PMID: 22434502) Theor Appl Genet. 2024 Jan 19;137(1):25. (PMID: 38240841) Plant Dis. 2017 Oct;101(10):1753-1760. (PMID: 30676927) Plant Cell. 1996 Jan;8(1):5-14. (PMID: 12239354) Annu Rev Phytopathol. 2025 Sep;63(1):147-173. (PMID: 40903423) Plant J. 2021 Jul;107(1):303-314. (PMID: 33893684) Front Plant Sci. 2023 Sep 18;14:1269498. (PMID: 37790783) Theor Appl Genet. 2023 Oct 25;136(11):232. (PMID: 37875655) Nat Genet. 2015 Dec;47(12):1494-8. (PMID: 26551671) Plant Dis. 2024 Jan;108(1):13-19. (PMID: 37526485) Mol Plant. 2020 Dec 7;13(12):1694-1708. (PMID: 32979565) BMC Biol. 2023 Feb 8;21(1):29. (PMID: 36755285) Gigascience. 2020 Jun 1;9(6):. (PMID: 32501478) Mol Biol Evol. 2013 Apr;30(4):772-80. (PMID: 23329690) Theor Appl Genet. 2025 Jul 19;138(8):185. (PMID: 40682612) Nature. 2020 Dec;588(7837):277-283. (PMID: 33239791) Genome Biol. 2014;15(12):550. (PMID: 25516281) Mol Plant. 2020 Dec 7;13(12):1733-1751. (PMID: 32896642) Plant Commun. 2025 Mar 10;6(3):101222. (PMID: 39690740) Nat Commun. 2024 Mar 19;15(1):2449. (PMID: 38503771) Plant Genome. 2025 Jun;18(2):e70029. (PMID: 40268757) Theor Appl Genet. 2024 Nov 6;137(12):261. (PMID: 39505770) Plant Physiol. 2024 Dec 23;197(1):. (PMID: 39405430) Science. 2009 Mar 6;323(5919):1360-3. (PMID: 19229000) J Biol Chem. 1999 Dec 3;274(49):34993-5004. (PMID: 10574976) Nature. 2003 Oct 30;425(6961):973-7. (PMID: 14586469) Phytopathology. 1998 Sep;88(9):890-4. (PMID: 18944865) Nat Genet. 2019 May;51(5):885-895. (PMID: 30962619) Mol Plant. 2023 Dec 4;16(12):1893-1910. (PMID: 37897037) Plant Biotechnol J. 2023 May;21(5):979-989. (PMID: 36650924) Plant Dis. 2009 Nov;93(11):1219. (PMID: 30754589) Theor Appl Genet. 2018 May;131(5):1063-1071. (PMID: 29392374) Plant Biotechnol J. 2025 Sep;23(9):3851-3863. (PMID: 40522813) Plant Sci. 2023 Oct;335:111785. (PMID: 37419327) Theor Appl Genet. 2023 May 31;136(6):146. (PMID: 37258797) Theor Appl Genet. 2025 Jun 12;138(7):148. (PMID: 40504369) Plant Biotechnol J. 2014 Aug;12(6):787-96. (PMID: 24646323) Mol Plant. 2021 Dec 6;14(12):1965-1968. (PMID: 34715393) Bioinformatics. 2015 Jun 15;31(12):2038-9. (PMID: 25649618) Nat Methods. 2015 Apr;12(4):357-60. (PMID: 25751142) Nature. 2022 Feb;602(7897):455-460. (PMID: 35140403) Theor Appl Genet. 2019 Nov;132(11):3009-3022. (PMID: 31317234) Theor Appl Genet. 2022 Mar;135(3):1083-1099. (PMID: 35006334) Nat Biotechnol. 2014 Sep;32(9):947-51. (PMID: 25038773) Theor Appl Genet. 2023 Aug 22;136(9):196. (PMID: 37606731) Front Genet. 2021 May 12;12:661742. (PMID: 34054924) J Exp Bot. 2023 May 19;74(10):3019-3032. (PMID: 36879436) |
| Grant Information: | 32360503 National Natural Science Foundation of China; 2023AAC05050 Natural Science Foundation of Ningxia Province; 2023BSB03059 Key Research and Development Program of Ningxia; 2025BBF01010 Key Research and Development Program of Ningxia; 2022YFD1200900 National Key Research and Development Project of China |
| Substance Nomenclature: | 0 (Genetic Markers) |
| Entry Date(s): | Date Created: 20261013 Date Completed: 20261013 Latest Revision: 20261016 |
| Update Code: | 20261016 |
| DOI: | 10.1007/s00122-026-05406-3 |
| PMID: | 42867689 |
| Database: | MEDLINE |
| ISSN: | 1432-2242 |
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| DOI: | 10.1007/s00122-026-05406-3 |