Academic Journal
Effects of probiotics on joint homeostasis and osteoarthritis: Development of microbe-gut-cartilage axis-on-a-chip.
| Τίτλος: | Effects of probiotics on joint homeostasis and osteoarthritis: Development of microbe-gut-cartilage axis-on-a-chip. |
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| Συγγραφείς: | Liu J; Department of Biomedical Engineering, The Chinese University of Hong Kong, Hong Kong SAR, China.; InnoHK Center for Neuromusculoskeletal Restorative Medicine, Hong Kong Science Park, Hong Kong SAR, China., Zhang N; Department of Biomedical Engineering, The Chinese University of Hong Kong, Hong Kong SAR, China.; Department of Orthopedic Surgery, Center for Orthopedic Surgery, The Third Affiliated Hospital, Southern Medical University, Guangzhou, China., Lei Y; Department of Biomedical Engineering, The Chinese University of Hong Kong, Hong Kong SAR, China.; Department of Orthopaedic Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China., Wang X; Department of Biomedical Engineering, The Chinese University of Hong Kong, Hong Kong SAR, China.; Department of Rehabilitation Medicine, The Third Affiliated Hospital, Southern Medical University, Guangzhou, Guangdong, China., Zhang X; Institute of Digestive Disease and Department of Medicine and Therapeutics, The State Key Laboratory of Digestive Disease, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Hong Kong SAR, China., Huang W; Department of Orthopaedic Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China., Ding C; Clinical Research Center, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong, China.; Clinical Research Centre, Beijing Tsinghua Changgung Hospital, Tsinghua Medicine, Tsinghua University, Beijing, China.; School of Biomedical Engineering, Tsinghua Medicine, Tsinghua University, Beijing, China., Xie D; Department of Orthopedic Surgery, Center for Orthopedic Surgery, The Third Affiliated Hospital, Southern Medical University, Guangzhou, China., Yu J; Institute of Digestive Disease and Department of Medicine and Therapeutics, The State Key Laboratory of Digestive Disease, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Hong Kong SAR, China., Tuan RS; Department of Biomedical Engineering, The Chinese University of Hong Kong, Hong Kong SAR, China.; InnoHK Center for Neuromusculoskeletal Restorative Medicine, Hong Kong Science Park, Hong Kong SAR, China.; Institute for Tissue Engineering and Regenerative Medicine, and School of Biomedical Sciences, The Chinese University of Hong Kong, Hong Kong SAR, China.; Peter Hung Pain Research Institute, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong SAR, China., Li ZA; Department of Biomedical Engineering, The Chinese University of Hong Kong, Hong Kong SAR, China.; InnoHK Center for Neuromusculoskeletal Restorative Medicine, Hong Kong Science Park, Hong Kong SAR, China.; Institute for Tissue Engineering and Regenerative Medicine, and School of Biomedical Sciences, The Chinese University of Hong Kong, Hong Kong SAR, China.; Peter Hung Pain Research Institute, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong SAR, China.; The State Key Laboratory of Digestive Disease, The Chinese University of Hong Kong, Hong Kong SAR, China. |
| Πηγή: | Science advances [Sci Adv] 2026 Oct 09; Vol. 12 (41), pp. eaeg8557. Date of Electronic Publication: 2026 Oct 09. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: American Association for the Advancement of Science Country of Publication: United States NLM ID: 101653440 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2375-2548 (Electronic) Linking ISSN: 23752548 NLM ISO Abbreviation: Sci Adv Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Washington, DC : American Association for the Advancement of Science, [2015]- |
| Ιατρικοί όροι (MeSH): | Probiotics*/pharmacology , Osteoarthritis*/metabolism , Osteoarthritis*/microbiology , Osteoarthritis*/pathology , Osteoarthritis*/drug therapy , Homeostasis*/drug effects , Joints*/metabolism , Joints*/drug effects , Cartilage*/metabolism , Cartilage*/drug effects , Lab-On-A-Chip Devices*, Organoids/metabolism ; Chondrocytes/metabolism ; Humans ; Microphysiological Systems ; Microbiota ; Animals ; Multiomics |
| Περίληψη: | Although emerging evidence implicates gut microbiome-cartilage cross-talk in the pathogenesis of osteoarthritis (OA), the most common joint disease, studying this microbe-gut-joint axis remains challenging due to limitations of animal models and conventional in vitro systems. Here, we report a microbe-gut-cartilage axis-on-a-chip (MGCAoC) that recreates tissue-specific microenvironments with oxygen gradients and physiological fluid shear. The computer simulation-optimized chip design generates an anoxic-oxic interface for the gut module and enables perfusion of probiotic metabolites to human cartilage organoids without cross-contamination. Multiomics profiling suggested that probiotic metabolites may promote cartilage anabolism, potentially involving PI3K and MAPK pathway, and this effect was associated with up-regulated collagen type II and aggrecan expression while suppressing metalloproteinases. In inflamed cartilage organoids, these metabolites were associated with reduced proinflammatory signaling while preserving matrix integrity. The chondroprotective effects were confirmed using cartilage organoids derived from patients with OA treated with identified metabolites. This MGCAoC enables mechanistic investigation of gut-joint axis physiology and provides a versatile preclinical tool for screening probiotic-based OA therapeutics. |
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| Entry Date(s): | Date Created: 20261009 Date Completed: 20261009 Latest Revision: 20261011 |
| Update Code: | 20261011 |
| PubMed Central ID: | PMC13651862 |
| DOI: | 10.1126/sciadv.aeg8557 |
| PMID: | 42853928 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 2375-2548 |
|---|---|
| DOI: | 10.1126/sciadv.aeg8557 |