Academic Journal
Phenotypic and genotypic assessment of antimicrobial and biocide resistance in enterobacteria from anaerobic digestion of dairy waste: implications for environmental and public health.
| Τίτλος: | Phenotypic and genotypic assessment of antimicrobial and biocide resistance in enterobacteria from anaerobic digestion of dairy waste: implications for environmental and public health. |
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| Συγγραφείς: | Alves MR; Graduate Program in Veterinary Sciences, Center for Agricultural Sciences and Engineering - CCAE, Federal University of Espírito Santo - UFES, Alto Universitario, no number, Guararema, Alegre, ES, 29500-000, Brazil., Ferreira NS; Graduate Program in Pharmaceutical Sciences, Center of Health Sciences, Federal University of Espírito Santo (UFES), Vitoria, Espírito Santo State, 29043-900, Brazil., Fontes MS; Department of Pharmacy and Nutrition, Federal University of Espírito Santo - UFES, Alegre, ES, 29500-000, Brazil., Lima MF; Department of Pharmacy and Nutrition, Federal University of Espírito Santo - UFES, Alegre, ES, 29500-000, Brazil., Otenio MH; Embrapa Dairy Cattle - Brazilian Agricultural Research Corporation, Juiz de Fora, Brazil., Ignacchiti MDC; Department of Pharmacy and Nutrition, Federal University of Espírito Santo - UFES, Alegre, ES, 29500-000, Brazil., Resende JA; Graduate Program in Veterinary Sciences, Center for Agricultural Sciences and Engineering - CCAE, Federal University of Espírito Santo - UFES, Alto Universitario, no number, Guararema, Alegre, ES, 29500-000, Brazil. juliana.resende@ufes.br.; Graduate Program in Pharmaceutical Sciences, Center of Health Sciences, Federal University of Espírito Santo (UFES), Vitoria, Espírito Santo State, 29043-900, Brazil. juliana.resende@ufes.br.; Department of Pharmacy and Nutrition, Federal University of Espírito Santo - UFES, Alegre, ES, 29500-000, Brazil. juliana.resende@ufes.br. |
| Πηγή: | World journal of microbiology & biotechnology [World J Microbiol Biotechnol] 2026 Oct 08; Vol. 42 (10). Date of Electronic Publication: 2026 Oct 08. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Springer Country of Publication: Germany NLM ID: 9012472 Publication Model: Electronic Cited Medium: Internet ISSN: 1573-0972 (Electronic) Linking ISSN: 09593993 NLM ISO Abbreviation: World J Microbiol Biotechnol Subsets: MEDLINE |
| Imprint Name(s): | Publication: 2005- : Berlin : Springer Original Publication: Oxford, OX, UK : Published by Rapid Communications of Oxford Ltd in association with UNESCO and in collaboration with the International Union of Microbiological Societies, c1990- |
| Ιατρικοί όροι (MeSH): | Disinfectants*/pharmacology , Enterobacteriaceae*/genetics , Enterobacteriaceae*/drug effects , Enterobacteriaceae*/isolation & purification , Drug Resistance, Bacterial*/genetics, Anti-Bacterial Agents/pharmacology ; Drug Resistance, Multiple, Bacterial/genetics ; Genes, Bacterial/genetics ; Anaerobiosis ; Genotype ; Phenotype ; Animals ; Microbial Sensitivity Tests ; Dairying ; Cattle |
| Περίληψη: | Antimicrobial-resistant bacteria in agricultural waste represent a critical One Health concern. Anaerobic digestion of livestock waste is widely promoted; however, the fate of antimicrobial resistance and biocide tolerance in full-scale systems remains unclear, constraining safe digestate reuse. This study assessed phenotypic and genotypic resistance in Escherichia coli-like enterobacteria isolated from effluent and biofertilizer in a full-scale dairy anaerobic digestion system. A total of 173 isolates were screened for antimicrobial resistance genes (ARGs), antimicrobial susceptibility, multidrug resistance (MDR), and biocide inhibition-zone responses. No diarrheagenic virulence genes were detected. At least one screened ARG was detected in 91.0% of effluent and 83.3% of biofertilizer isolates. qacE∆1 was the most frequent marker and occurred significantly more often in effluent (70.8%) than biofertilizer isolates (44.0%; q = 0.00291). Phenotypic resistance to at least one antimicrobial was observed in 27.0% and 34.5% of effluent and biofertilizer isolates, respectively, while MDR occurred in 2.2% and 9.5% (p = 0.0521). tet(a) carriage was strongly associated with tetracycline resistance (OR = 20.9; q < 0.001). H (© 2026. The Author(s).) |
| Competing Interests: | Declarations. Ethics Approval: Not applicable. This study analyzed environmental samples collected from a dairy-waste anaerobic digestion system and did not involve experimental procedures performed on humans or animals. Competing interests: The authors declare no competing interests. |
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| Grant Information: | FAPES; TO 326/2022 Fundação de Amparo à Pesquisa e Inovação do Espírito Santo |
| Contributed Indexing: | Keywords: Anaerobic digestion; Antimicrobial resistance; Biocide; Dairy cattle; Enterobacterales |
| Substance Nomenclature: | 0 (Disinfectants) 0 (Anti-Bacterial Agents) |
| Entry Date(s): | Date Created: 20261008 Date Completed: 20261008 Latest Revision: 20261011 |
| Update Code: | 20261011 |
| PubMed Central ID: | PMC13649801 |
| DOI: | 10.1007/s11274-026-05319-9 |
| PMID: | 42848244 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1573-0972 |
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| DOI: | 10.1007/s11274-026-05319-9 |