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Single-cell RNA sequencing unravels mosquito biology and host-pathogen interactions.

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Τίτλος: Single-cell RNA sequencing unravels mosquito biology and host-pathogen interactions.
Συγγραφείς: Liu LB; Guizhou Key Laboratory of Microbio and Infectious Disease Prevention and Control, School of Basic Medicine, Guizhou Medical University, Guiyang, 561113, Guizhou, China.; The Key and Characteristic Laboratory of Modern Pathogen Biology, School of Basic Medicine, Guizhou Medical University, Guiyang, 561113, Guizhou, China.; Institute of Virology, School of Basic Medicine, Guizhou Medical University, Guiyang, 561113, Guizhou, China., Ye HB; Guizhou Key Laboratory of Microbio and Infectious Disease Prevention and Control, School of Basic Medicine, Guizhou Medical University, Guiyang, 561113, Guizhou, China.; The Key and Characteristic Laboratory of Modern Pathogen Biology, School of Basic Medicine, Guizhou Medical University, Guiyang, 561113, Guizhou, China.; Institute of Virology, School of Basic Medicine, Guizhou Medical University, Guiyang, 561113, Guizhou, China., Tian ZH; Guizhou Key Laboratory of Microbio and Infectious Disease Prevention and Control, School of Basic Medicine, Guizhou Medical University, Guiyang, 561113, Guizhou, China.; The Key and Characteristic Laboratory of Modern Pathogen Biology, School of Basic Medicine, Guizhou Medical University, Guiyang, 561113, Guizhou, China.; Institute of Virology, School of Basic Medicine, Guizhou Medical University, Guiyang, 561113, Guizhou, China., Zeng XH; Guizhou Key Laboratory of Microbio and Infectious Disease Prevention and Control, School of Basic Medicine, Guizhou Medical University, Guiyang, 561113, Guizhou, China.; The Key and Characteristic Laboratory of Modern Pathogen Biology, School of Basic Medicine, Guizhou Medical University, Guiyang, 561113, Guizhou, China.; Institute of Virology, School of Basic Medicine, Guizhou Medical University, Guiyang, 561113, Guizhou, China., Wu JH; Guizhou Key Laboratory of Microbio and Infectious Disease Prevention and Control, School of Basic Medicine, Guizhou Medical University, Guiyang, 561113, Guizhou, China. jiahongwu2008@hotmail.com.; The Key and Characteristic Laboratory of Modern Pathogen Biology, School of Basic Medicine, Guizhou Medical University, Guiyang, 561113, Guizhou, China. jiahongwu2008@hotmail.com.; Institute of Virology, School of Basic Medicine, Guizhou Medical University, Guiyang, 561113, Guizhou, China. jiahongwu2008@hotmail.com.
Πηγή: Parasites & vectors [Parasit Vectors] 2026 Oct 02; Vol. 19 (1). Date of Electronic Publication: 2026 Oct 02.
Τύπος έκδοσης: Journal Article; Review
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: BioMed Central Country of Publication: England NLM ID: 101462774 Publication Model: Electronic Cited Medium: Internet ISSN: 1756-3305 (Electronic) Linking ISSN: 17563305 NLM ISO Abbreviation: Parasit Vectors Subsets: MEDLINE
Imprint Name(s): Original Publication: London : BioMed Central
Ιατρικοί όροι (MeSH): Host-Pathogen Interactions*/genetics , Culicidae*/genetics , Culicidae*/physiology , Culicidae*/parasitology , Single-Cell Analysis*/methods , Sequence Analysis, RNA*/methods , Mosquito Vectors*/genetics , Mosquito Vectors*/physiology, Animals ; Single-Cell Gene Expression Analysis ; Gene Expression Profiling ; Mosquito-Borne Diseases
Περίληψη: Single-cell RNA sequencing (scRNA-seq) is fundamentally reshaping the research paradigm of mosquito biology and host-pathogen interactions via its high-resolution transcriptomic profiling capability. This review first outlines the technical workflow and data analysis strategies of scRNA-seq, then highlights key applications of scRNA-seq in constructing mosquito cell atlases, deciphering post-blood-meal transcriptional remodeling, sexual dimorphism, and receptor coexpression patterns. Furthermore, the role of scRNA-seq in revealing the cellular tropism of mosquito-borne pathogens, host immune responses, and the identification of potential intervention targets is discussed. Despite current challenges such as the lack of specific cell markers and insufficient functional validation, scRNA-seq has provided a novel perspective for understanding mosquito biology at the single-cell level and laid an important foundation for developing targeted vector control strategies. Looking forward, integration with spatial transcriptomics, multiomics approaches, and dynamic experimental validation will further advance this field toward mechanistic research and translational applications.
(© 2026. The Author(s).)
Competing Interests: Declarations. Ethics approval and consent to participate: Not applicable. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests.
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Grant Information: 82502712 National Natural Science Foundation of China; QKHJC[2024]Youth219 Guizhou Provincial Basic Research Program (Natural Science); 2024YFC2607801 National Key Research and Development Program of China; Qiankehe[2025]024 Central-Guided Local Science and Technology Projects of Guizhou Province, China; Qian Ke He Platform Talent-CXTD[2022]004 Science and Technology Innovation Talent Team of Guizhou Province
Contributed Indexing: Keywords: Cell atlas; Host–pathogen interactions; Mosquito; Mosquito-borne disease; Single-cell RNA sequencing; Vector control
Entry Date(s): Date Created: 20261002 Date Completed: 20261002 Latest Revision: 20261005
Update Code: 20261005
PubMed Central ID: PMC13632198
DOI: 10.1186/s13071-026-07460-8
PMID: 42827263
Βάση Δεδομένων: MEDLINE
Περιγραφή
ISSN:1756-3305
DOI:10.1186/s13071-026-07460-8