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Effects of Post-infection Supplementation with Lactiplantibacillus plantarum CRD7 and Lacticaseibacillus rhamnosus CRD11 on Helicobacter pylori Virulence Gene Expression in a Rat Model of Gastric Infection.

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Τίτλος: Effects of Post-infection Supplementation with Lactiplantibacillus plantarum CRD7 and Lacticaseibacillus rhamnosus CRD11 on Helicobacter pylori Virulence Gene Expression in a Rat Model of Gastric Infection.
Συγγραφείς: Seddighi NS; Department of Biology, Da.C., Islamic Azad University, Damghan, Iran., Asl AAH; Department of Biology, Da.C., Islamic Azad University, Damghan, Iran., Karam MRA; Department of Molecular Biology, Pasteur Institute of Iran, Tehran, Iran., Khatibi SR; Department of Epidemiology and Biostatistics, School of Health, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran. khatibiser@gmail.com., Azizi O; Public Health Research Center, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran. Azizi-omid@outlook.com.; Research Center of Advanced Technologies in Medicine, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran. Azizi-omid@outlook.com.
Πηγή: Current microbiology [Curr Microbiol] 2026 Oct 02; Vol. 83 (11). Date of Electronic Publication: 2026 Oct 02.
Τύπος έκδοσης: Journal Article
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: Springer International Country of Publication: United States NLM ID: 7808448 Publication Model: Electronic Cited Medium: Internet ISSN: 1432-0991 (Electronic) Linking ISSN: 03438651 NLM ISO Abbreviation: Curr Microbiol Subsets: MEDLINE
Imprint Name(s): Original Publication: New York, Springer International.
Ιατρικοί όροι (MeSH): Probiotics*/administration & dosage , Helicobacter Infections*/microbiology , Helicobacter Infections*/therapy , Helicobacter pylori*/genetics , Helicobacter pylori*/pathogenicity , Lacticaseibacillus rhamnosus*/physiology , Virulence Factors*/genetics , Lactiplantibacillus plantarum*/physiology, Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Antigens, Bacterial/genetics ; Animals ; Male ; Rats ; Disease Models, Animal ; Rats, Wistar
Περίληψη: Helicobacter pylori infects nearly half of the global population and causes gastritis through virulence factors such as CagA and VacA. Increasing antibiotic resistance has reduced the effectiveness of conventional eradication therapies, highlighting the need for alternative strategies, including probiotics. This study evaluated the effects of post-infection supplementation with Lactiplantibacillus plantarum, Lacticaseibacillus rhamnosus, or their combination on cagA and vacA mRNA expression in a rat model of H. pylori infection. Male Wistar rats (n = 40) were randomly assigned to five groups (n = 8 each), including uninfected controls, H. pylori-infected untreated rats, and infected groups receiving L. plantarum CRD7, L. rhamnosus CRD11, or their combination. Infection was established by oral gavage and confirmed by rapid urease testing, bacterial isolation, and quantitative colony counting. Probiotics were administered orally after infection, and gastric tissues were collected for RNA extraction. Expression levels of cagA and vacA were quantified by qRT-PCR using the 2^(-ΔΔCt) method. H. pylori infection significantly increased cagA (2.7 ± 0.6-fold) and vacA (7.2 ± 0.7-fold) expression compared with controls. Probiotic supplementation reduced cagA expression to 1.86 ± 0.43-fold with L. rhamnosus, 1.2 ± 0.45-fold with L. plantarum, and 0.7 ± 0.8-fold with the combined treatment. Similarly, vacA expression decreased to 3.6 ± 0.44-fold, 1.2 ± 0.6-fold, and 1.7 ± 0.8-fold, respectively. One-way ANOVA showed a trend toward reduced cagA expression (p = 0.08) and significant differences in vacA expression among groups (p < 0.001). Post-infection supplementation with L. plantarum CRD7 and L. rhamnosus CRD11, particularly in combination, attenuated the expression of key H. pylori virulence-associated genes and may represent potential adjunctive strategies for modulating H. pylori virulence-associated gene expression.
(© 2026. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Competing Interests: Declarations. Competing interest: The authors declare no competing interests. Consent for Publication: Not applicable. Consent for Participate: Not applicable. Ethics Approval: All animal experiments were approved by the Ethics Committee of Torbat Heydariyeh University of Medical Sciences (THUMS), Iran (ethical code: IR.THUMS.AEC.1401.001).
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Substance Nomenclature: 0 (Bacterial Proteins)
0 (VacA protein, Helicobacter pylori)
0 (Virulence Factors)
0 (Antigens, Bacterial)
0 (cagA protein, Helicobacter pylori)
Entry Date(s): Date Created: 20261002 Date Completed: 20261002 Latest Revision: 20261030
Update Code: 20261030
DOI: 10.1007/s00284-026-05112-9
PMID: 42827167
Βάση Δεδομένων: MEDLINE
Περιγραφή
ISSN:1432-0991
DOI:10.1007/s00284-026-05112-9