Academic Journal
Recent progress in microbial production of D-aspartate.
| Τίτλος: | Recent progress in microbial production of D-aspartate. |
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| Συγγραφείς: | Imanishi D; Department of Materials Science and Bioengineering, Nagaoka University of Technology, Nagaoka, 940-2188, Niigata, Japan., Pham LV; Department of Materials Science and Bioengineering, Nagaoka University of Technology, Nagaoka, 940-2188, Niigata, Japan., Takahashi S; Department of Materials Science and Bioengineering, Nagaoka University of Technology, Nagaoka, 940-2188, Niigata, Japan. shoutaka@vos.nagaokaut.ac.jp. |
| Πηγή: | World journal of microbiology & biotechnology [World J Microbiol Biotechnol] 2026 Oct 01; Vol. 42 (10). Date of Electronic Publication: 2026 Oct 01. |
| Τύπος έκδοσης: | Journal Article; Review |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Springer Country of Publication: Germany NLM ID: 9012472 Publication Model: Electronic Cited Medium: Internet ISSN: 1573-0972 (Electronic) Linking ISSN: 09593993 NLM ISO Abbreviation: World J Microbiol Biotechnol Subsets: MEDLINE |
| Imprint Name(s): | Publication: 2005- : Berlin : Springer Original Publication: Oxford, OX, UK : Published by Rapid Communications of Oxford Ltd in association with UNESCO and in collaboration with the International Union of Microbiological Societies, c1990- |
| Ιατρικοί όροι (MeSH): | D-Aspartic Acid*/biosynthesis , D-Aspartic Acid*/metabolism , Bacteria*/metabolism , Bacteria*/genetics , Aspartic Acid*/biosynthesis, Amino Acid Isomerases/metabolism ; Asparaginase/metabolism ; D-Aspartate Oxidase/metabolism ; Industrial Microbiology ; Fermentation ; Metabolic Engineering |
| Περίληψη: | D-Aspartate (D-Asp) is a bioactive compound involved in neuroendocrine regulation and reproductive function in animals, including humans, with tissue-protective effects reported in experimental models, and has potential applications as a raw material for pharmaceuticals and functional food ingredients. It is also used as a side-chain precursor for semisynthetic antibiotics. An established industrial process for D-Asp uses enzymatic conversion by immobilized microbial cells with chemically synthesized D,L-Asp as the starting material and is constrained by dependence on chemical feedstock synthesis. Sustainable alternatives have therefore attracted attention: whole-cell bioconversion of exogenously supplied L-Asp has been demonstrated, whereas de novo production from a renewable carbon source has not yet been reported. This mini-review briefly notes the physiological and industrial contexts that motivate microbial production, and then focuses on the functions, biosynthesis, metabolism, and transport of D-Asp in microorganisms. We further discuss the screening of high-D-Asp-producing microorganisms using a high-throughput colorimetric assay based on D-aspartate oxidase, together with the molecular and cultivation characteristics of the strains identified. These studies indicate that racemization mediated by aspartate racemase, precursor supply via L-asparaginase, competing metabolism associated with bifunctional aspartate aminotransferase, and pH control during cultivation influence extracellular D-Asp accumulation; the contribution of membrane transport remains unresolved. Finally, we highlight the identification of D-Asp exporters, metabolic engineering of production strains, and optimization of cultivation processes as key future priorities for establishing a sustainable and reproducible fermentation platform for D-Asp production. (© 2026. The Author(s).) |
| Competing Interests: | Declarations. Ethics approval and consent to participate: Not applicable. Competing interests: The authors declare no competing interests. Generative AI and AI-assisted technologies: During the preparation of this work, the authors used Claude (Anthropic) and ChatGPT/Codex (OpenAI) to assist with literature organization, drafting, and translation. After using these tools, the authors reviewed and edited the content as needed and take full responsibility for the content of the published article. |
| References: | Cell Mol Life Sci. 2011 Mar;68(5):817-31. (PMID: 21161322) Bioresour Technol. 2017 Dec;245(Pt B):1588-1602. (PMID: 28579173) Biochim Biophys Acta. 1991 Jul 12;1078(3):377-82. (PMID: 1907199) Chem Soc Rev. 2021 May 24;50(10):5952-5984. (PMID: 34027955) Biosci Biotechnol Biochem. 1993 Jul;57(7):1145-8. (PMID: 7763985) Res Microbiol. 2024 Sep-Oct;175(7):104219. (PMID: 38945250) Extremophiles. 2016 Jul;20(4):385-93. (PMID: 27094682) J Biochem. 2004 Apr;135(4):533-40. (PMID: 15115779) Metab Eng. 2022 Nov;74:121-129. (PMID: 36341775) J Food Drug Anal. 2017 Jul;25(3):699-708. (PMID: 28911655) J Biol Chem. 2011 Aug 19;286(33):29044-29052. (PMID: 21719707) J Am Chem Soc. 1947 Jul;69(7):1674. (PMID: 20251401) Science. 2010 Apr 30;328(5978):627-9. (PMID: 20431016) Appl Environ Microbiol. 2022 Aug 9;88(15):e0088322. (PMID: 35862682) Biol Pharm Bull. 2015;38(2):298-305. (PMID: 25747990) Enzyme Microb Technol. 2021 Sep;149:109835. (PMID: 34311880) Metab Eng. 2019 Jul;54:244-254. (PMID: 31063790) Appl Microbiol Biotechnol. 2015 Apr;99(8):3341-9. (PMID: 25758960) Int J Mol Sci. 2020 Nov 18;21(22):. (PMID: 33218144) Water Sci Technol. 2022 Jan;85(1):116-124. (PMID: 35050870) Ecotoxicol Environ Saf. 2023 Jul 1;259:115067. (PMID: 37244200) FEBS J. 2025 Sep;292(17):4395-4417. (PMID: 40146632) Biotechnol Lett. 2025 Dec 15;48(1):12. (PMID: 41398542) Microb Biotechnol. 2025 Jun;18(6):e70179. (PMID: 40491232) J Biol Chem. 2015 May 22;290(21):13079-94. (PMID: 25847251) J Mol Biol. 2002 May 31;319(2):479-89. (PMID: 12051922) Biotechnol Bioeng. 2021 Jul;118(7):2493-2502. (PMID: 33760222) Science. 2009 Sep 18;325(5947):1552-5. (PMID: 19762646) J Biol Chem. 1996 Feb 9;271(6):3079-84. (PMID: 8621704) Appl Microbiol Biotechnol. 2022 Apr;106(7):2651-2663. (PMID: 35305124) Amino Acids. 2017 Sep;49(9):1521-1533. (PMID: 28681245) J Bacteriol. 2009 Jun;191(11):3752-7. (PMID: 19329637) Anim Nutr. 2018 Sep;4(3):311-315. (PMID: 30175260) Sci Rep. 2025 Jul 13;15(1):25328. (PMID: 40653503) J Bacteriol. 2013 Dec;195(23):5391-5. (PMID: 24097941) Fertil Steril. 2005 Nov;84(5):1444-9. (PMID: 16275242) Biosci Microbiota Food Health. 2023;42(4):254-263. (PMID: 37791341) J Biol Chem. 2006 Apr 28;281(17):11586-94. (PMID: 16510449) Biomolecules. 2019 Sep 03;9(9):. (PMID: 31484465) FEBS J. 2022 Oct;289(19):5933-5946. (PMID: 35377552) J Bacteriol. 2018 Jul 10;200(15):. (PMID: 29437849) Commun Biol. 2025 Sep 25;8(1):1359. (PMID: 40998974) FEMS Microbiol Rev. 2020 Sep 1;44(5):538-564. (PMID: 32495833) J Psychiatr Res. 2013 Oct;47(10):1432-7. (PMID: 23835041) Springerplus. 2013 Dec 27;2:691. (PMID: 24422181) Biochem Biophys Res Commun. 2019 Jul 5;514(4):1108-1114. (PMID: 31101340) Biochem Biophys Rep. 2021 Jul 08;27:101073. (PMID: 34286112) Cells. 2024 Aug 22;13(16):. (PMID: 39195288) J Bacteriol. 2007 Oct;189(19):7089-97. (PMID: 17660287) |
| Grant Information: | 25K08877 Grant-in-Aid for Scientific Research (C) |
| Contributed Indexing: | Keywords: Latilactobacillus curvatus; d-Aspartate; Aspartate racemase; High-throughput screening; Lactic acid bacteria; Microbial production |
| Substance Nomenclature: | 4SR0Q8YD1X (D-Aspartic Acid) EC 5.1.1.- (Amino Acid Isomerases) EC 5.1.1.13 (aspartate racemase) 30KYC7MIAI (Aspartic Acid) EC 3.5.1.1 (Asparaginase) EC 1.4.3.1 (D-Aspartate Oxidase) |
| Entry Date(s): | Date Created: 20261001 Date Completed: 20261001 Latest Revision: 20261004 |
| Update Code: | 20261004 |
| PubMed Central ID: | PMC13631041 |
| DOI: | 10.1007/s11274-026-05296-z |
| PMID: | 42821137 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1573-0972 |
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| DOI: | 10.1007/s11274-026-05296-z |