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Light-induced remodeling of the Drosophila phosphoproteome regulates visual adaptation through the Trp channel.

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Τίτλος: Light-induced remodeling of the Drosophila phosphoproteome regulates visual adaptation through the Trp channel.
Συγγραφείς: Kramer LD; Department of Biochemistry, Purdue University, West Lafayette, IN 47907, USA., Brandwine-Shemmer T; Department of Medical Neurobiology Faculty of Medicine and Edmond and Lily Safra Center for Brain Sciences (ELSC), Hebrew University, Jerusalem, Israel., Pierce LR; Department of Biochemistry, Purdue University, West Lafayette, IN 47907, USA., Liu YK; Department of Biochemistry, Purdue University, West Lafayette, IN 47907, USA., Bartels JP; Institute of Biology, Department of Biochemistry, University of Hohenheim, 70599 Stuttgart, Germany., Huber A; Institute of Biology, Department of Biochemistry, University of Hohenheim, 70599 Stuttgart, Germany., Minke B; Department of Medical Neurobiology Faculty of Medicine and Edmond and Lily Safra Center for Brain Sciences (ELSC), Hebrew University, Jerusalem, Israel., Tao WA; Department of Biochemistry, Purdue University, West Lafayette, IN 47907, USA.; Purdue Center for Cancer Research, Purdue University, West Lafayette, IN 47907, USA.; Department of Chemistry, Purdue University, West Lafayette, IN 47907, USA., Weake VM; Department of Biochemistry, Purdue University, West Lafayette, IN 47907, USA.; Purdue Center for Cancer Research, Purdue University, West Lafayette, IN 47907, USA.
Πηγή: Science signaling [Sci Signal] 2026 Sep 29; Vol. 19 (957), pp. eaei4151. Date of Electronic Publication: 2026 Sep 29.
Τύπος έκδοσης: Journal Article
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: American Association for the Advancement of Science Country of Publication: United States NLM ID: 101465400 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1937-9145 (Electronic) Linking ISSN: 19450877 NLM ISO Abbreviation: Sci Signal Subsets: MEDLINE
Imprint Name(s): Original Publication: Washington, D.C. : American Association for the Advancement of Science
Ιατρικοί όροι (MeSH): Drosophila Proteins*/metabolism , Drosophila Proteins*/genetics , Phosphoproteins*/metabolism , Phosphoproteins*/genetics , Proteome*/metabolism , Transient Receptor Potential Channels*/metabolism , Transient Receptor Potential Channels*/genetics , Drosophila melanogaster*/metabolism , Adaptation, Ocular*/physiology , Light*, Phosphorylation/radiation effects ; Protein Kinase C/metabolism ; Animals ; Blue Light
Περίληψη: Light is an important environmental stimulus that is necessary for vision and circadian entrainment. Here, we present a system-wide analysis of the kinase-mediated light response in Drosophila using optimized data-independent acquisition mass spectrometry (DIA-MS) for quantitative phosphoproteomics analysis. Exposure to standard white light or high-intensity blue light induced a widespread increase in phosphorylation relative to dark-adapted flies. The light-sensitive phosphoproteins were part of multiple pathways, including phototransduction, circadian control, lipid metabolism, and synapse organization, and exhibited distinct phosphorylation signatures under dark adaptation or white or blue light. The Ca2+-permeable transient receptor potential (Trp) channel was differentially phosphorylated at multiple sites in a white and blue light-dependent manner, and phosphorylation stoichiometric analysis identified Thr849 in Trp as a pivotal functional switch. It was entirely unphosphorylated in the dark, but 80% of the Trp molecules were phosphorylated in either light condition. This phosphorylation event was mediated in part by the eye-specific protein kinase C InaC and regulated the eye's electrophysiological response to oscillating light. Thus, our results show that light remodels the Drosophila phosphoproteome, establishing a crucial mechanism for sensory adaptation to a changing light-dark environment.
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Grant Information: R01 EY033734 United States EY NEI NIH HHS
Substance Nomenclature: 0 (Drosophila Proteins)
0 (Phosphoproteins)
0 (Proteome)
0 (Transient Receptor Potential Channels)
0 (trp protein, Drosophila)
EC 2.7.11.13 (Protein Kinase C)
Entry Date(s): Date Created: 20260929 Date Completed: 20260929 Latest Revision: 20261023
Update Code: 20261023
PubMed Central ID: PMC13688266
DOI: 10.1126/scisignal.aei4151
PMID: 42809632
Βάση Δεδομένων: MEDLINE
Περιγραφή
ISSN:1937-9145
DOI:10.1126/scisignal.aei4151