Academic Journal
Light-induced remodeling of the Drosophila phosphoproteome regulates visual adaptation through the Trp channel.
| Τίτλος: | Light-induced remodeling of the Drosophila phosphoproteome regulates visual adaptation through the Trp channel. |
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| Συγγραφείς: | Kramer LD; Department of Biochemistry, Purdue University, West Lafayette, IN 47907, USA., Brandwine-Shemmer T; Department of Medical Neurobiology Faculty of Medicine and Edmond and Lily Safra Center for Brain Sciences (ELSC), Hebrew University, Jerusalem, Israel., Pierce LR; Department of Biochemistry, Purdue University, West Lafayette, IN 47907, USA., Liu YK; Department of Biochemistry, Purdue University, West Lafayette, IN 47907, USA., Bartels JP; Institute of Biology, Department of Biochemistry, University of Hohenheim, 70599 Stuttgart, Germany., Huber A; Institute of Biology, Department of Biochemistry, University of Hohenheim, 70599 Stuttgart, Germany., Minke B; Department of Medical Neurobiology Faculty of Medicine and Edmond and Lily Safra Center for Brain Sciences (ELSC), Hebrew University, Jerusalem, Israel., Tao WA; Department of Biochemistry, Purdue University, West Lafayette, IN 47907, USA.; Purdue Center for Cancer Research, Purdue University, West Lafayette, IN 47907, USA.; Department of Chemistry, Purdue University, West Lafayette, IN 47907, USA., Weake VM; Department of Biochemistry, Purdue University, West Lafayette, IN 47907, USA.; Purdue Center for Cancer Research, Purdue University, West Lafayette, IN 47907, USA. |
| Πηγή: | Science signaling [Sci Signal] 2026 Sep 29; Vol. 19 (957), pp. eaei4151. Date of Electronic Publication: 2026 Sep 29. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: American Association for the Advancement of Science Country of Publication: United States NLM ID: 101465400 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1937-9145 (Electronic) Linking ISSN: 19450877 NLM ISO Abbreviation: Sci Signal Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Washington, D.C. : American Association for the Advancement of Science |
| Ιατρικοί όροι (MeSH): | Drosophila Proteins*/metabolism , Drosophila Proteins*/genetics , Phosphoproteins*/metabolism , Phosphoproteins*/genetics , Proteome*/metabolism , Transient Receptor Potential Channels*/metabolism , Transient Receptor Potential Channels*/genetics , Drosophila melanogaster*/metabolism , Adaptation, Ocular*/physiology , Light*, Phosphorylation/radiation effects ; Protein Kinase C/metabolism ; Animals ; Blue Light |
| Περίληψη: | Light is an important environmental stimulus that is necessary for vision and circadian entrainment. Here, we present a system-wide analysis of the kinase-mediated light response in Drosophila using optimized data-independent acquisition mass spectrometry (DIA-MS) for quantitative phosphoproteomics analysis. Exposure to standard white light or high-intensity blue light induced a widespread increase in phosphorylation relative to dark-adapted flies. The light-sensitive phosphoproteins were part of multiple pathways, including phototransduction, circadian control, lipid metabolism, and synapse organization, and exhibited distinct phosphorylation signatures under dark adaptation or white or blue light. The Ca2+-permeable transient receptor potential (Trp) channel was differentially phosphorylated at multiple sites in a white and blue light-dependent manner, and phosphorylation stoichiometric analysis identified Thr849 in Trp as a pivotal functional switch. It was entirely unphosphorylated in the dark, but 80% of the Trp molecules were phosphorylated in either light condition. This phosphorylation event was mediated in part by the eye-specific protein kinase C InaC and regulated the eye's electrophysiological response to oscillating light. Thus, our results show that light remodels the Drosophila phosphoproteome, establishing a crucial mechanism for sensory adaptation to a changing light-dark environment. |
| Substance Nomenclature: | 0 (Drosophila Proteins) 0 (Phosphoproteins) 0 (Proteome) 0 (Transient Receptor Potential Channels) 0 (trp protein, Drosophila) EC 2.7.11.13 (Protein Kinase C) |
| Entry Date(s): | Date Created: 20260929 Date Completed: 20260929 Latest Revision: 20260929 |
| Update Code: | 20260930 |
| DOI: | 10.1126/scisignal.aei4151 |
| PMID: | 42809632 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1937-9145 |
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| DOI: | 10.1126/scisignal.aei4151 |