Academic Journal
Thymidine kinase 1 activity in serum and body cavity fluids of horses with lymphoma.
| Τίτλος: | Thymidine kinase 1 activity in serum and body cavity fluids of horses with lymphoma. |
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| Συγγραφείς: | Drozdzewska K; Equine Clinic, Freie Universität Berlin, Berlin, Germany.; Roseworthy Veterinary Hospital, School of Animal and Veterinary Sciences, Adelaide University, Roseworthy, Australia., Burde S; Laboklin Laboratory for Clinical Diagnostics GmbH & Co. KG, Bad Kissingen, Germany., Müller E; Laboklin Laboratory for Clinical Diagnostics GmbH & Co. KG, Bad Kissingen, Germany., Merle R; Institute of Veterinary Epidemiology and Biostatistics, Freie Universität Berlin, Berlin, Germany., Gehlen H; Equine Clinic, Freie Universität Berlin, Berlin, Germany. |
| Πηγή: | Journal of veterinary internal medicine [J Vet Intern Med] 2026 Sep 01; Vol. 40 (5). |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Wiley Periodicals Country of Publication: England NLM ID: 8708660 Publication Model: Print Cited Medium: Internet ISSN: 1939-1676 (Electronic) Linking ISSN: 08916640 NLM ISO Abbreviation: J Vet Intern Med Subsets: MEDLINE |
| Imprint Name(s): | Publication: <2010-> : Malden, MA : Wiley Periodicals Original Publication: Philadelphia : J.B. Lippincott Co., [c1987- |
| Ιατρικοί όροι (MeSH): | Thymidine Kinase*/blood , Thymidine Kinase*/metabolism , Lymphoma*/veterinary , Lymphoma*/enzymology , Lymphoma*/blood , Horse Diseases*/enzymology , Horse Diseases*/blood , Body Fluids*/enzymology, Biomarkers, Tumor/blood ; Animals ; Horses ; Male ; Female ; Cross-Sectional Studies |
| Περίληψη: | Background: The accuracy of serum thymidine kinase 1 (TK1S) as a biomarker of lymphoma in horses is variable. The value of measurement in body cavity fluid (TK1F) has not been established. Hypothesis/objectives: (1) TK1F and TK1S will be higher in horses with lymphoma compared to diseased controls (DCs); (2) TK1F will be higher than TK1S; and (3) there will be a strong correlation between both measurements. Animals: Twenty animals in the lymphoma and 31 animals with nonneoplastic, gastrointestinal/respiratory disorders in DC group. Methods: This was a cross-sectional study using prospectively collected data and samples obtained during routine diagnostic evaluation at referral hospitals. Thymidine kinase 1 was measured using chemiluminescence immunoassay and presented as median (and interquartile range). Nonparametric tests compared TK1F and TK1S between and within groups and Kendall's Tau assessed correlation between TK1F and TK1S. Results: Median TK1F was 7.48 (2.20-48.23) U/L in horses with lymphoma vs 2.50 (0.59-4.83) U/L in the DC group (P = .007); median TK1S was 4.26 (0.46-9.37) U/L vs 0.40 (0.40-0.75) U/L (P < .001), respectively. Both biomarkers showed good discrimination (area under the curve > 0.70) between lymphoma and the DC group, with optimal cutoff values of 5.7 and 2.5 U/L, respectively. Thymidine kinase 1 activity in body cavity fluid (3.62 [0.85-9.86] U/L) was significantly higher than TK1S (0.50 [0.40-2.09] U/L; P < .001) and there was no significant correlation between them within groups. Conclusions and Clinical Importance: Thymidine kinase 1 activity in body cavity fluid and TK1S provide different information and should be interpreted independently. They proved useful in lymphoma diagnosis, but not as a standalone test. (© The Author(s) 2026. Published by Oxford University Press on behalf of the American College of Veterinary Internal Medicine.) |
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| Grant Information: | Laboklin Laboratory for Clinical Diagnostics GmbH & Co. KG, Germany; Freie Universität Berlin |
| Contributed Indexing: | Keywords: TK1; chemiluminescence immunoassay; horse; lymphosarcoma; oncology; peritoneal fluid |
| Substance Nomenclature: | EC 2.7.1.21 (Thymidine Kinase) EC 2.7.1.21 (thymidine kinase 1) 0 (Biomarkers, Tumor) |
| Entry Date(s): | Date Created: 20260926 Date Completed: 20260926 Latest Revision: 20260928 |
| Update Code: | 20260928 |
| PubMed Central ID: | PMC13615731 |
| DOI: | 10.1093/jvimsj/aalag207 |
| PMID: | 42800046 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1939-1676 |
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| DOI: | 10.1093/jvimsj/aalag207 |