In Vitro Effects of an Olive Polyphenol-Bromelain Formulation on U87MG Glioblastoma Cells: Viability, Cell-Cycle Distribution, Cytokine Release, and Total Antioxidant Capacity.

Λεπτομέρειες βιβλιογραφικής εγγραφής
Τίτλος:	In Vitro Effects of an Olive Polyphenol-Bromelain Formulation on U87MG Glioblastoma Cells: Viability, Cell-Cycle Distribution, Cytokine Release, and Total Antioxidant Capacity.
Συγγραφείς:	Ciraci E; Department of Biochemistry, Faculty of Pharmacy, Biruni University, Zeytinburnu, 34010 Istanbul, Türkiye.; Biruni Advanced Technology Application and Research Center (BAMER), Biruni University, 34015 Istanbul, Türkiye., Elgun T; Biruni Advanced Technology Application and Research Center (BAMER), Biruni University, 34015 Istanbul, Türkiye.; Department of Medical Biology, Faculty of Medicine, Biruni University, Zeytinburnu, 34015 Istanbul, Türkiye., Oltulu YM; Biruni Advanced Technology Application and Research Center (BAMER), Biruni University, 34015 Istanbul, Türkiye.; Department of Medical Biology, Faculty of Medicine, Biruni University, Zeytinburnu, 34015 Istanbul, Türkiye., Arslan HI; Biruni Advanced Technology Application and Research Center (BAMER), Biruni University, 34015 Istanbul, Türkiye., Yurttas AG; Department of Biochemistry, Faculty of Medicine, Istanbul Atlas University, 34408 Istanbul, Türkiye., Gundogan GI; Biruni Advanced Technology Application and Research Center (BAMER), Biruni University, 34015 Istanbul, Türkiye.; Department of Histology and Embryology, Faculty of Medicine, Biruni University, 34015 Istanbul, Türkiye., Uzun L; Department of Chemistry, Faculty of Science, Hacettepe University, 06800 Ankara, Türkiye., Emekdas G; Turk Kan Vakfi, 34718 Istanbul, Türkiye.
Πηγή:	Molecules (Basel, Switzerland) [Molecules] 2026 Sep 20; Vol. 31 (18). Date of Electronic Publication: 2026 Sep 20.
Τύπος έκδοσης:	Journal Article
Γλώσσα:	English
Στοιχεία περιοδικού:	Publisher: MDPI Country of Publication: Switzerland NLM ID: 100964009 Publication Model: Electronic Cited Medium: Internet ISSN: 1420-3049 (Electronic) Linking ISSN: 14203049 NLM ISO Abbreviation: Molecules Subsets: MEDLINE
Imprint Name(s):	Original Publication: Basel, Switzerland : MDPI, c1995-
Ιατρικοί όροι (MeSH):	Polyphenols/pharmacology , Polyphenols/chemistry , Antioxidants/pharmacology , Antioxidants/chemistry , Glioblastoma/metabolism , Glioblastoma/drug therapy , Glioblastoma/pathology , Olea/chemistry , Cytokines*/metabolism, Cell Survival/drug effects ; Cell Cycle/drug effects ; Humans ; Cell Line, Tumor ; Tandem Mass Spectrometry
Περίληψη:	Background: Glioblastoma (GBM) remains difficult to treat, and natural bioactive formulations are being explored as experimental modifiers of tumor-associated cellular processes. This study examined a fixed-ratio olive polyphenol-bromelain formulation in U87MG cells and performed targeted phenolic characterization of its olive-derived component. Methods: Seven phenolics were quantified by LC-MS/MS. U87MG cells were exposed to the formulation or a bromelain comparator. The MTT metabolic signal, DNA-content cell-cycle distribution, IL-6, IL-10, and extracellular total antioxidant capacity (TAC) were assessed. Results: The total quantified phenolics were 483.22 mg/kg, with oleocanthal, oleuropein, and 3,4-DHPEA-EDA predominating. The formulation reduced the MTT signal more than bromelain at lower and intermediate concentrations, although the response became non-monotonic at higher concentrations. Combination-treated cells showed greater G0/G1 accumulation. Culture supernatants showed lower IL-6 and higher IL-10 and TAC relative to untreated controls. Conclusions: The formulation was associated with changes in metabolic activity, cell-cycle distribution, and extracellular cytokine/redox readouts in U87MG cells. Because no polyphenol-only arm, direct cell-death assay, complete replicate-level inferential analysis, or non-tumoral comparator was available, the findings are descriptive and do not establish synergy, cytotoxicity, mechanism, tumor selectivity, or therapeutic efficacy.
References:	Nat Commun. 2025 Nov 19;16(1):10151. (PMID: 41258298) Front Cell Dev Biol. 2022 Sep 12;10:1011435. (PMID: 36172282) Crit Rev Food Sci Nutr. 2002;42(3):209-21. (PMID: 12058980) Nat Med. 2018 May;24(5):541-550. (PMID: 29686425) Neuro Oncol. 2025 Nov 1;27(11):2751-2788. (PMID: 40827022) NPJ Precis Oncol. 2024 Oct 23;8(1):241. (PMID: 39443641) Food Sci Nutr. 2025 Sep 18;13(9):e70976. (PMID: 40979569) Anticancer Res. 2020 Oct;40(10):5427-5436. (PMID: 32988864) Front Oncol. 2023 Jan 09;12:1068778. (PMID: 36698404) PLoS One. 2019 Jan 18;14(1):e0210274. (PMID: 30657763) Semin Cancer Biol. 2016 Oct;40-41:82-99. (PMID: 27117759) Int J Mol Sci. 2025 Nov 01;26(21):. (PMID: 41226697) Radiology. 2022 Sep;304(3):494-508. (PMID: 35880978) JCO Oncol Pract. 2023 Apr;19(4):180-189. (PMID: 36638331) Neoplasia. 2001 Nov-Dec;3(6):469-79. (PMID: 11774029) Cell Mol Immunol. 2024 Dec;21(12):1354-1375. (PMID: 39406966) Nutrients. 2024 Jun 28;16(13):. (PMID: 38999808) PLoS One. 2010 Apr 16;5(4):e10202. (PMID: 20419137) Expert Rev Mol Med. 2021 Dec 02;23:e20. (PMID: 34852856) PLoS One. 2011;6(6):e20849. (PMID: 21695150) Clin Cancer Res. 2019 Jun 15;25(12):3643-3657. (PMID: 30824583) Food Funct. 2023 Sep 19;14(18):8101-8128. (PMID: 37650738) Cytokine Growth Factor Rev. 2025 Oct;85:26-42. (PMID: 39893129) Mol Carcinog. 2012 Mar;51(3):231-43. (PMID: 21432909) Molecules. 2020 Nov 14;25(22):. (PMID: 33202681) Pharmacogn Mag. 2015 Apr-Jun;11(42):404-9. (PMID: 25829782) Cancer Res. 2010 Jan 15;70(2):440-6. (PMID: 20068163) Curr Issues Mol Biol. 2025 Aug 18;47(8):. (PMID: 40864821) Cancer Res. 2026 Jul 15;86(14):3570-3587. (PMID: 41974019) Neuro Oncol. 2021 Aug 2;23(8):1231-1251. (PMID: 34185076)
Contributed Indexing:	Keywords: G0/G1 accumulation; IL-10; IL-6; U87MG; bromelain; cell-cycle distribution; glioblastoma; olive polyphenols; total antioxidant capacity
Substance Nomenclature:	0 (Polyphenols) 0 (Antioxidants) 0 (Cytokines)
Entry Date(s):	Date Created: 20260926 Date Completed: 20260926 Latest Revision: 20260929
Update Code:	20260929
PubMed Central ID:	PMC13610031
DOI:	10.3390/molecules31183334
PMID:	42796617
Βάση Δεδομένων:	MEDLINE

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ISSN:	1420-3049
DOI:	10.3390/molecules31183334