4-Hydroxy-3-Methyl-2-Alkenylquinoline Disrupts Temporospatial Control of Flagellar Assembly and Induces Transcriptomic Remodeling in Bacillus subtilis Consistent with a Short-Circuiting of the Spo0A Phosphorelay.

Λεπτομέρειες βιβλιογραφικής εγγραφής
Τίτλος:	4-Hydroxy-3-Methyl-2-Alkenylquinoline Disrupts Temporospatial Control of Flagellar Assembly and Induces Transcriptomic Remodeling in Bacillus subtilis Consistent with a Short-Circuiting of the Spo0A Phosphorelay.
Συγγραφείς:	Williams MD; Department of Biology, Texas A&M University, College Station, TX 77843, USA.; Department of Biology, Prairie View A&M University, Prairie View, TX 77446, USA., Smith L; Department of Biology, Texas A&M University, College Station, TX 77843, USA.; Antimicrobial Division, Sano Chemicals Inc., Bryan, TX 77808, USA.
Πηγή:	Molecules (Basel, Switzerland) [Molecules] 2026 Sep 12; Vol. 31 (18). Date of Electronic Publication: 2026 Sep 12.
Τύπος έκδοσης:	Journal Article
Γλώσσα:	English
Στοιχεία περιοδικού:	Publisher: MDPI Country of Publication: Switzerland NLM ID: 100964009 Publication Model: Electronic Cited Medium: Internet ISSN: 1420-3049 (Electronic) Linking ISSN: 14203049 NLM ISO Abbreviation: Molecules Subsets: MEDLINE
Imprint Name(s):	Original Publication: Basel, Switzerland : MDPI, c1995-
Ιατρικοί όροι (MeSH):	Bacillus subtilis/drug effects , Bacillus subtilis/genetics , Bacillus subtilis/metabolism , Flagella/drug effects , Flagella/metabolism , Flagella/genetics , Bacterial Proteins/metabolism , Bacterial Proteins/genetics , Transcription Factors/metabolism , Transcription Factors/genetics , Transcriptome/drug effects , Quinolines/pharmacology , Quinolines*/chemistry, Gene Expression Regulation, Bacterial/drug effects ; Phosphorylation/drug effects
Περίληψη:	4-hydroxy-3-methyl-2-alkenylquinolines (HMAQs) belong to an emerging sub-class of naturally synthesized alkaloids with several prominent biological properties. HMAQ-7 is a novel variant recently identified in Burkholderia contaminans MS14 which was previously shown to impede normal cellular behaviors in Bacillus subtilis, including multiple colonization phenotypes such as motility, pellicle formation, and sporulation. While the list of biological and chemical characteristics among HMAQ's and related molecules is extensive, the mechanistic basis of these responses has been far more elusive. Using a combination of RNA-seq and bioinformatics, we have determined that several of the physiological defects induced by HMAQ-7 are strongly correlated with alterations in systems related to phospho-transduction. Specifically, HMAQ-7 augments gene expression in elements associated with late stationary phase growth, including those involved in phosphate assimilation and regulation of the spo0A phosphorelay. We also note that HMAQ-7 promotes dysregulated transcription of flagellar assembly genes which would normally be downregulated during late stationary phase. This study constitutes one of the first extensive investigations into an HMAQ's mode of action, providing vital insight into the ecological dynamics between B. contaminans and B. subtilis.
References:	Antibiotics (Basel). 2021 Feb 02;10(2):. (PMID: 33540653) J Nat Prod. 2013 Feb 22;76(2):150-6. (PMID: 23394257) Chem Biol. 2007 Jan;14(1):87-96. (PMID: 17254955) Proc Natl Acad Sci U S A. 2010 May 4;107(18):8486-91. (PMID: 20404177) Mol Microbiol. 2015 Apr;96(2):325-48. (PMID: 25598361) Phytopathology. 2023 Jan;113(1):11-20. (PMID: 35913221) Front Microbiol. 2015 Sep 09;6:950. (PMID: 26441884) Appl Environ Microbiol. 2006 Jan;72(1):937-41. (PMID: 16391139) Expert Opin Drug Discov. 2021 Jul;16(7):807-822. (PMID: 33467922) J Bacteriol. 2026 Jun 18;208(6):e0058925. (PMID: 42133690) Sci Adv. 2026 Jul 17;12(29):eadz1707. (PMID: 42455896) J Bacteriol. 1991 Apr;173(8):2625-32. (PMID: 1901572) Bioinformatics. 2012 Aug 15;28(16):2184-5. (PMID: 22743226) J Bacteriol. 2008 Aug;190(15):5339-52. (PMID: 18539738) Bioinformatics. 2014 Apr 1;30(7):923-30. (PMID: 24227677) J Bacteriol. 2014 Dec;196(23):4184-96. (PMID: 25225273) J Bacteriol. 2014 Jan;196(1):16-27. (PMID: 24123822) Front Microbiol. 2017 Jun 20;8:1021. (PMID: 28676791) Int J Toxicol. 2012 Jul-Aug;31(4):326-36. (PMID: 22689636) Curr Opin Microbiol. 2005 Apr;8(2):203-7. (PMID: 15802253) Mol Microbiol. 2001 Dec;42(5):1199-209. (PMID: 11886552) Front Cell Infect Microbiol. 2018 Jul 04;8:230. (PMID: 30023354) J Mol Biol. 1989 Dec 5;210(3):551-9. (PMID: 2693738) Nat Methods. 2013 Apr;10(4):325-7. (PMID: 23435259) J Bacteriol. 2000 Jun;182(12):3446-51. (PMID: 10852876) J Bacteriol. 2005 Feb;187(4):1357-68. (PMID: 15687200) Front Cell Infect Microbiol. 2019 Feb 28;9:33. (PMID: 30873388) Pathogens. 2017 Dec 25;7(1):. (PMID: 29295589) Mol Microbiol. 2013 Oct;90(1):6-21. (PMID: 23888912) Genome Biol. 2014;15(12):550. (PMID: 25516281) Biochimie. 1992 Jul-Aug;74(7-8):619-26. (PMID: 1391039) J Bacteriol. 1975 Jul;123(1):366-71. (PMID: 806579) Curr Opin Microbiol. 2009 Apr;12(2):182-91. (PMID: 19249239) Bioinformatics. 2012 Oct 15;28(20):2678-9. (PMID: 22914218) Antimicrob Agents Chemother. 2018 Nov 26;62(12):. (PMID: 30275083) Cell Chem Biol. 2017 Dec 21;24(12):1437-1444.e3. (PMID: 29033316) J Nat Prod. 2020 Jul 24;83(7):2145-2154. (PMID: 32631063) Cell Mol Life Sci. 2002 Mar;59(3):392-402. (PMID: 11964117) J Biosci Bioeng. 2006 Aug;102(2):110-7. (PMID: 17027872) Nucleic Acids Res. 2019 Jul 2;47(W1):W191-W198. (PMID: 31066453) Annu Rev Genet. 2014;48:319-40. (PMID: 25251856) J Biochem. 1998 Jul;124(1):98-104. (PMID: 9644251) Appl Environ Microbiol. 2011 Sep;77(17):6189-98. (PMID: 21742901) Gigascience. 2021 Feb 16;10(2):. (PMID: 33590861) mBio. 2024 Sep 11;15(9):e0164224. (PMID: 39152718) Antimicrob Agents Chemother. 2020 Jul 22;64(8):. (PMID: 32457108) Antimicrob Agents Chemother. 2020 Nov 17;64(12):. (PMID: 32958713) mBio. 2010 May 18;1(1):. (PMID: 20689749) Microbiology (Reading). 2020 Feb;166(2):169-179. (PMID: 31860435) Pest Manag Sci. 2019 Aug;75(8):2264-2270. (PMID: 30701660) J Bacteriol. 2006 Jun;188(12):4442-52. (PMID: 16740951) mBio. 2019 May 21;10(3):. (PMID: 31113899) Cell Death Dis. 2015 Jan 22;6:e1609. (PMID: 25611384) J Proteome Res. 2025 Aug 1;24(8):4082-4097. (PMID: 40680064) Bioinformatics. 2016 Oct 1;32(19):3047-8. (PMID: 27312411) Mol Microbiol. 2025 Apr;123(4):305-329. (PMID: 39812382) J Bacteriol. 2004 Sep;186(18):6003-14. (PMID: 15342569) J Bacteriol. 1989 Jun;171(6):3095-101. (PMID: 2498284) Bioinformatics. 2013 Jul 15;29(14):1830-1. (PMID: 23740750) Mol Microbiol. 2003 Oct;50(1):101-4. (PMID: 14507367) Eur J Med Chem. 2017 Sep 29;138:491-500. (PMID: 28689097) J Bacteriol. 2006 Jul;188(14):5273-85. (PMID: 16816200) FEMS Microbiol Lett. 2018 May 1;365(9):. (PMID: 29718276) Appl Environ Microbiol. 2013 May;79(9):2899-905. (PMID: 23435879) Appl Environ Microbiol. 2020 Nov 24;86(24):. (PMID: 33008823) BMC Microbiol. 2015 Sep 29;15:192. (PMID: 26420312) Bioinformatics. 2013 Jan 1;29(1):15-21. (PMID: 23104886) BMC Bioinformatics. 2016 Oct 21;17(1):428. (PMID: 27769170) Curr Opin Microbiol. 2016 Dec;34:38-46. (PMID: 27501460) Nat Biotechnol. 2017 Apr 11;35(4):316-319. (PMID: 28398311) Front Microbiol. 2016 Jun 28;7:1025. (PMID: 27446060) Front Microbiol. 2016 Mar 18;7:275. (PMID: 27047450) Molecules. 2020 Dec 02;25(23):. (PMID: 33276615) J Gen Microbiol. 1989 May;135(5):1335-45. (PMID: 2516118) Genes Dev. 2005 Dec 15;19(24):3083-94. (PMID: 16357223) Gels. 2023 Sep 29;9(10):. (PMID: 37888361) mSphere. 2025 May 27;10(5):e0108124. (PMID: 40338090) Appl Environ Microbiol. 2008 Mar;74(6):1798-804. (PMID: 18245256) Molecules. 2023 Feb 08;28(4):. (PMID: 36838633) J Bacteriol. 2024 Aug 22;206(8):e0013324. (PMID: 39012109) Mol Microbiol. 2020 Aug;114(2):279-291. (PMID: 32259388) Mol Microbiol. 2000 Nov;38(3):535-42. (PMID: 11069677) Antibiotics (Basel). 2022 Aug 23;11(9):. (PMID: 36139923) Curr Opin Microbiol. 2026 Aug;92:102791. (PMID: 42413152) J Bacteriol. 2011 Nov;193(22):6366-74. (PMID: 21949067) Nat Microbiol. 2026 Jul;11(7):1920-1933. (PMID: 42174289) J Bacteriol. 1992 May;174(9):2771-8. (PMID: 1569009)
Grant Information:	R41 AI131792 United States AI NIAID NIH HHS; R42 AI131792 United States AI NIAID NIH HHS; R41AI131792 National Institute of Allergy and Infectious Diseases; R42AI131792 National Institute of Allergy and Infectious Diseases
Contributed Indexing:	Keywords: Bacillus subtilis; Burkholderia; Spo0A; alkaloid; anti-biofilm; anti-sporulation; antimicrobial; flagella; quinolone
Substance Nomenclature:	0 (Bacterial Proteins) 0 (Spo0A protein, Bacillus subtilis) 0 (Transcription Factors) 0 (Quinolines)
Entry Date(s):	Date Created: 20260926 Date Completed: 20260926 Latest Revision: 20260930
Update Code:	20260930
PubMed Central ID:	PMC13609334
DOI:	10.3390/molecules31183226
PMID:	42796513
Βάση Δεδομένων:	MEDLINE

Full Text Finder

Περιγραφή
ISSN:	1420-3049
DOI:	10.3390/molecules31183226