Academic Journal
BSA-Seq-Based QTL Mapping for the Height of the First Fruiting Branch Node of Cotton and the Development of Molecular Markers.
| Τίτλος: | BSA-Seq-Based QTL Mapping for the Height of the First Fruiting Branch Node of Cotton and the Development of Molecular Markers. |
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| Συγγραφείς: | Zhao F; Cotton Research Institute, Xinjiang Academy of Agricultural and Reclamation Science/Northwest Inland Region Key Laboratory of Cotton Biology and Genetic Breeding, Ministry of Agriculture and Rural Affairs, Shihezi 832000, China., Yang T; Cotton Research Institute, Xinjiang Academy of Agricultural and Reclamation Science/Northwest Inland Region Key Laboratory of Cotton Biology and Genetic Breeding, Ministry of Agriculture and Rural Affairs, Shihezi 832000, China.; College of Modern Agriculture and Food Engineering, Xinjiang Vocational University of Technology, Kashi 844004, China., Wang X; Cotton Research Institute, Xinjiang Academy of Agricultural and Reclamation Science/Northwest Inland Region Key Laboratory of Cotton Biology and Genetic Breeding, Ministry of Agriculture and Rural Affairs, Shihezi 832000, China., Wang G; Cotton Research Institute, Xinjiang Academy of Agricultural and Reclamation Science/Northwest Inland Region Key Laboratory of Cotton Biology and Genetic Breeding, Ministry of Agriculture and Rural Affairs, Shihezi 832000, China., Qiao J; Cotton Research Institute, Xinjiang Academy of Agricultural and Reclamation Science/Northwest Inland Region Key Laboratory of Cotton Biology and Genetic Breeding, Ministry of Agriculture and Rural Affairs, Shihezi 832000, China., Kong X; Tumushuke Agri-Tech R&D Center, Xinjiang Academy of Agricultural Reclamation Sciences, Tumushuke 843900, China., Liu L; Cotton Research Institute, Xinjiang Academy of Agricultural and Reclamation Science/Northwest Inland Region Key Laboratory of Cotton Biology and Genetic Breeding, Ministry of Agriculture and Rural Affairs, Shihezi 832000, China., Han W; Cotton Research Institute, Xinjiang Academy of Agricultural and Reclamation Science/Northwest Inland Region Key Laboratory of Cotton Biology and Genetic Breeding, Ministry of Agriculture and Rural Affairs, Shihezi 832000, China., Yu Y; Cotton Research Institute, Xinjiang Academy of Agricultural and Reclamation Science/Northwest Inland Region Key Laboratory of Cotton Biology and Genetic Breeding, Ministry of Agriculture and Rural Affairs, Shihezi 832000, China. |
| Πηγή: | Genes [Genes (Basel)] 2026 Aug 28; Vol. 17 (9). Date of Electronic Publication: 2026 Aug 28. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: MDPI Country of Publication: Switzerland NLM ID: 101551097 Publication Model: Electronic Cited Medium: Internet ISSN: 2073-4425 (Electronic) Linking ISSN: 20734425 NLM ISO Abbreviation: Genes (Basel) Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Basel : MDPI |
| Ιατρικοί όροι (MeSH): | Quantitative Trait Loci*/genetics , Gossypium*/genetics , Gossypium*/growth & development, Chromosome Mapping/methods ; Whole Genome Sequencing/methods ; Genetic Markers ; Phenotype ; Genome, Plant ; Polymorphism, Single Nucleotide ; Plant Breeding |
| Περίληψη: | The height of the first fruiting branch node (HFFBN) is a core indicator for mechanical harvesting of cotton, and the development of molecular markers for this trait is important for accelerating the breeding process. In this study, using bulked segregant analysis coupled with whole-genome sequencing (BSA-seq), one quantitative trait locus (QTL) associated with the HFFBN was mapped; a molecular marker, qFBH7, associated with the HFFBN of cotton was developed; and its application value was systematically evaluated. A total of 20 lines with extreme phenotypes were selected from the recombinant inbred lines constructed using upland cotton Z3-146 and Z3-147 as parental lines. The screened lines with extreme phenotypes were used to construct the extreme high-HFFBN pool and the extreme low-HFFBN pool, which were subsequently used for BSA-seq. Using the upland cotton genome as a reference, relevant QTLs were mapped by BSA-seq. One relevant candidate region was identified, with a total length of 2.25 Mb. The validation experiments revealed that the genotyping results of the KASP_FBH7_03 molecular marker in the parental lines Z3-146 and Z3-147 were completely consistent with the BSA-seq data: Z3-146 had the TT genotype, and Z3-147 had the CC genotype. Among the 66 samples from the natural population, there was a significant difference (p < 0.05) in the HFFBN between the CC and TT genotypes, and the mean HFFBN of the TT genotype was greater than that of the CC genotype. In summary, the KASP_FBH7_03 molecular marker can be effectively used for selective breeding for the HFFBN of cotton, and the TT genotype has a positive regulatory effect on the HFFBN. This study not only provides resources for breeding cotton varieties suited to mechanical harvesting but also offers a robust tool for molecular marker-assisted selection. |
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| Grant Information: | GMLM2023 Winall Hi-tech Seed Co.,Ltd.; State Key Laboratory of Cotton Bio-breeding and Integrated Utilization; CB2024A20 State Key Laboratory of Cotton Bio-breeding and Integrated Utilization Open Fund; 2025SNGGGCC-BT01 Germplasm Innovation and New Variety Breeding of Drought-Tolerant, Salt-Tolerant, and Machine-Harvestable Cotton |
| Contributed Indexing: | Keywords: SNP-based genotyping; bulked segregant analysis; fruiting branch node height; quantitative trait loci; upland cotton |
| Substance Nomenclature: | 0 (Genetic Markers) |
| Entry Date(s): | Date Created: 20260926 Date Completed: 20260926 Latest Revision: 20260929 |
| Update Code: | 20260929 |
| PubMed Central ID: | PMC13606609 |
| DOI: | 10.3390/genes17091030 |
| PMID: | 42792924 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 2073-4425 |
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| DOI: | 10.3390/genes17091030 |