Academic Journal
High-resolution cryoEM of nucleosomes in nuclear extracts of mammalian cells.
| Τίτλος: | High-resolution cryoEM of nucleosomes in nuclear extracts of mammalian cells. |
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| Συγγραφείς: | Ker DS; Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, U.K., Aboalnaga H; Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, U.K., Pellegrini L; Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, U.K. |
| Πηγή: | Bioscience reports [Biosci Rep] 2026 Oct 21; Vol. 46 (10). |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Portland Press on behalf of the Biochemical Society Country of Publication: England NLM ID: 8102797 Publication Model: Print Cited Medium: Internet ISSN: 1573-4935 (Electronic) Linking ISSN: 01448463 NLM ISO Abbreviation: Biosci Rep Subsets: MEDLINE |
| Imprint Name(s): | Publication: London : Portland Press on behalf of the Biochemical Society Original Publication: London : The Biochemical Society, c1981- |
| Ιατρικοί όροι (MeSH): | Nucleosomes*/ultrastructure , Nucleosomes*/chemistry , Cryoelectron Microscopy*/methods , Cell Nucleus*/ultrastructure, Humans |
| Περίληψη: | Frontier structural biology methods are transitioning from analysis of reconstituted macromolecular complexes in vitro to imaging of macromolecular assemblies within the physiological confines of the cell. Preparation of samples for in situ cryoEM analysis requires focused ion beam (FIB) milling or ultramicrotome sectioning, laborious and technically challenging procedures that are low-throughput and require a high degree of technical skill. We have devised a simple approach for cryoEM of nuclear macromolecular complexes that preserves to a high degree their physiological environment while removing the need for thin sectioning of the sample. The method requires only the preparation of nuclear extracts without additional purification or enrichment steps. We applied the method to obtain a 2.3 Å cryoEM structure of nucleosomes visualised directly in the nuclear lysate of human cells. (© 2026 The Author(s).) |
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| Grant Information: | United Kingdom WT_ Wellcome Trust; 221892/Z/20/Z Wellcome Trust (WT) |
| Contributed Indexing: | Keywords: chromatin; cryoem; nuclear extraction; nucleosome; single-particle analysis; structural biology |
| Substance Nomenclature: | 0 (Nucleosomes) |
| Entry Date(s): | Date Created: 20260924 Date Completed: 20261006 Latest Revision: 20261009 |
| Update Code: | 20261009 |
| PubMed Central ID: | PMC13643017 |
| DOI: | 10.1042/BSR20260477 |
| PMID: | 42782067 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1573-4935 |
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| DOI: | 10.1042/BSR20260477 |