Λεπτομέρειες βιβλιογραφικής εγγραφής
| Τίτλος: |
Improving CTC isolation by controlling orbit engagement in inertial microfluidics. |
| Συγγραφείς: |
Ghera CF; Department of Biomedical Engineering, University of Illinois Chicago, Chicago, IL, USA. papauts@uic.edu., Hay R; Institute for Multiscale Thermofluids, University of Edinburgh, UK., Zhou J; Department of Cardiovascular and Thoracic Surgery, Rush University Medical Center, Chicago, IL 60612, USA.; Department of Anatomy and Cell Biology, Rush University Medical Center, Chicago, IL 60612, USA., Owen B; Institute for Multiscale Thermofluids, University of Edinburgh, UK., Papautsky I; Department of Biomedical Engineering, University of Illinois Chicago, Chicago, IL, USA. papauts@uic.edu.; University of Illinois Cancer Center, Chicago, IL, USA. |
| Πηγή: |
Lab on a chip [Lab Chip] 2026 Oct 13; Vol. 26 (20), pp. 5443-5458. Date of Electronic Publication: 2026 Oct 13. |
| Τύπος έκδοσης: |
Journal Article |
| Γλώσσα: |
English |
| Στοιχεία περιοδικού: |
Publisher: Royal Society of Chemistry Country of Publication: England NLM ID: 101128948 Publication Model: Electronic Cited Medium: Internet ISSN: 1473-0189 (Electronic) Linking ISSN: 14730189 NLM ISO Abbreviation: Lab Chip Subsets: MEDLINE |
| Imprint Name(s): |
Original Publication: Cambridge, UK : Royal Society of Chemistry, c2001- |
| Ιατρικοί όροι (MeSH): |
Neoplastic Cells, Circulating*/pathology , Cell Separation*/instrumentation , Cell Separation*/methods , Microfluidic Analytical Techniques*/instrumentation , Microfluidic Analytical Techniques*/methods , Lab-On-A-Chip Devices*, Humans ; Cell Line, Tumor ; Equipment Design |
| Περίληψη: |
Inertial microfluidics provides a label-free strategy for isolating circulating tumor cells (CTCs) from blood by exploiting size-dependent lateral migration in microchannels. However, straight-channel devices are often optimized using the final equilibrium position, even though target-cell loss can arise from incomplete or poorly reproducible migration before outlet collection. Here, we show that heteroclinic orbit engagement provides a predictive design criterion for improving CTC scale separation in a rectangular co-flow inertial microchannel. A computational model predicted size-dependent heteroclinic orbits and lateral migration velocities, which were experimentally validated using beads under controlled inlet flow-split conditions. Increasing buffer confinement shifted the sample-buffer interface closer to the sidewall, promoted earlier orbit engagement, improved agreement between measured and simulated velocity profiles, and reduced upstream trajectory variability. Larger CTC scale particles engaged their orbits earlier and migrated more reproducibly, while smaller white blood cell (WBC) scale particles remained laterally displaced from the larger particle focusing region. Biological validation with PANC1 and A549 cells showed that the 1 : 6 : 1 flow-split configuration increased separation efficiency to 99.9 ± 0.2% for PANC1 and maintained high separation efficiency of 99.2 ± 0.9% for A549 cells, although its effect on total recovery was cell line dependent. In an intraductal papillary mucinous neoplasm (IPMN) patient sample, representing a premalignant pancreatic lesion that may progress to invasive pancreatic ductal adenocarcinoma (PDAC), CTC target outlet partitioning was 67% under 1 : 1 : 1 and 100% under 1 : 6 : 1, within the limits of the detected cell numbers. These results establish orbit engagement as a mechanistic design principle for improving clinically relevant CTC enrichment without changing device geometry, total flow rate, or labeling strategy. |
| Entry Date(s): |
Date Created: 20260924 Date Completed: 20261013 Latest Revision: 20261013 |
| Update Code: |
20261014 |
| DOI: |
10.1039/d6lc00633g |
| PMID: |
42781935 |
| Βάση Δεδομένων: |
MEDLINE |