Machine vision-enabled OD600 measurement and mass spectrometric determination of metabolites of microbial cell-containing microfluidic droplets in real-time.

Λεπτομέρειες βιβλιογραφικής εγγραφής
Τίτλος:	Machine vision-enabled OD600 measurement and mass spectrometric determination of metabolites of microbial cell-containing microfluidic droplets in real-time.
Συγγραφείς:	Knecht GT; Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109, USA. rtkenn@umich.edu., Unger MJ; Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109, USA. rtkenn@umich.edu.; Life Sciences Institute, University of Michigan, Ann Arbor, Michigan 48109, USA., Narayan ARH; Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109, USA. rtkenn@umich.edu.; Life Sciences Institute, University of Michigan, Ann Arbor, Michigan 48109, USA.; Program in Chemical Biology, University of Michigan, Ann Arbor, Michigan 48109, USA., Kennedy RT; Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109, USA. rtkenn@umich.edu.
Πηγή:	The Analyst [Analyst] 2026 Oct 13; Vol. 151 (20), pp. 6036-6046. Date of Electronic Publication: 2026 Oct 13.
Τύπος έκδοσης:	Journal Article
Γλώσσα:	English
Στοιχεία περιοδικού:	Publisher: Royal Society of Chemistry Country of Publication: England NLM ID: 0372652 Publication Model: Electronic Cited Medium: Internet ISSN: 1364-5528 (Electronic) Linking ISSN: 00032654 NLM ISO Abbreviation: Analyst Subsets: MEDLINE
Imprint Name(s):	Publication: Cambridge : Royal Society of Chemistry Original Publication: London : Chemical Society
Ιατρικοί όροι (MeSH):	Escherichia coli/metabolism , Escherichia coli/growth & development , Escherichia coli/cytology , Mass Spectrometry/methods , Microfluidic Analytical Techniques/methods , Microfluidic Analytical Techniques/instrumentation
Περίληψη:	Droplet microfluidics has become a versatile tool for high-throughput phenotypic analysis of cells, employed in enzyme engineering, drug screening, and antibiotic assays. An important metric in such bioanalyses is the optical density (OD600) of the culture, but current methods for measuring OD600 within cultured droplets are limited. Herein, we present a machine-vision method for the measurement of OD600 between 0.1 and 4.3 in flowing microdroplets from 0.5-50 nL in volume. This system can make real-time decisions based on cell density and volume measurements and was demonstrated to remove vacant droplets from an emulsion by dielectrophoretic sorting at up to 10 droplets per s based on cell density with an error rate below 10%. We show that droplets can also be subjected to subsequent on-line analysis such as mass spectrometry (MS) and their growth levels correlated to metabolite abundances. We show that growth level is correlated with lysine abundance in droplets incubated with engineered E. coli cells.
Grant Information:	R01 GM150948 United States GM NIGMS NIH HHS
Entry Date(s):	Date Created: 20260924 Date Completed: 20261013 Latest Revision: 20261013
Update Code:	20261014
PubMed Central ID:	PMC13602199
DOI:	10.1039/d6an00623j
PMID:	42781733
Βάση Δεδομένων:	MEDLINE

Περιγραφή
ISSN:	1364-5528
DOI:	10.1039/d6an00623j