Academic Journal
Artemisinins inhibited the synergistic cariogenic effect between Candida albicans and Streptococcus mutans.
| Τίτλος: | Artemisinins inhibited the synergistic cariogenic effect between Candida albicans and Streptococcus mutans. |
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| Συγγραφείς: | Zhou J; State Key Laboratory of Oral Diseases, National Center for Stomatology, National Clinical Research Center for Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu, Sichuan, China.; West China Lecheng Hospital, Sichuan University, Boao, Hainan, China., Zhou Z; State Key Laboratory of Oral Diseases, National Center for Stomatology, National Clinical Research Center for Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu, Sichuan, China., Shen J; State Key Laboratory of Oral Diseases, National Center for Stomatology, National Clinical Research Center for Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu, Sichuan, China., Lin Y; State Key Laboratory of Oral Diseases, National Center for Stomatology, National Clinical Research Center for Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu, Sichuan, China., Lei L; State Key Laboratory of Oral Diseases, National Center for Stomatology, National Clinical Research Center for Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu, Sichuan, China., Gou L; State Key Laboratory of Oral Diseases, National Center for Stomatology, National Clinical Research Center for Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu, Sichuan, China., Meng Z; Department of Clinical Laboratory, West China School of Stomatology, Sichuan University, Chengdu, Sichuan, China., Zhou Y; Guangdong Provincial Key Laboratory of Stomatology, Guanghua School of Stomatology, Sun Yat-Sen University, Guangzhou, China., Liao B; State Key Laboratory of Oral Diseases, National Center for Stomatology, National Clinical Research Center for Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu, Sichuan, China., Cheng L; State Key Laboratory of Oral Diseases, National Center for Stomatology, National Clinical Research Center for Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu, Sichuan, China.; Department of Cariology and Endodontics, West China School of Stomatology, Sichuan University, Chengdu, Sichuan, China., Zhou X; State Key Laboratory of Oral Diseases, National Center for Stomatology, National Clinical Research Center for Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu, Sichuan, China.; Department of Cariology and Endodontics, West China School of Stomatology, Sichuan University, Chengdu, Sichuan, China., Ren B; State Key Laboratory of Oral Diseases, National Center for Stomatology, National Clinical Research Center for Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu, Sichuan, China. |
| Πηγή: | Frontiers in cellular and infection microbiology [Front Cell Infect Microbiol] 2026 Sep 08; Vol. 16, pp. 1950360. Date of Electronic Publication: 2026 Sep 08 (Print Publication: 2026). |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Frontiers Media SA Country of Publication: Switzerland NLM ID: 101585359 Publication Model: eCollection Cited Medium: Internet ISSN: 2235-2988 (Electronic) Linking ISSN: 22352988 NLM ISO Abbreviation: Front Cell Infect Microbiol Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Lausanne : Frontiers Media SA |
| Ιατρικοί όροι (MeSH): | Candida albicans*/drug effects , Candida albicans*/growth & development , Candida albicans*/physiology , Artemisinins*/pharmacology , Streptococcus mutans*/drug effects , Streptococcus mutans*/growth & development , Streptococcus mutans*/genetics , Dental Caries*/microbiology , Dental Caries*/prevention & control , Dental Caries*/drug therapy , Microbial Interactions*/drug effects, Biofilms/drug effects ; Biofilms/growth & development ; Virulence/drug effects ; Hyphae/drug effects ; Hyphae/growth & development ; Anti-Bacterial Agents/pharmacology ; Animals ; Drug Synergism ; Disease Models, Animal ; Rats ; Microbial Sensitivity Tests |
| Περίληψη: | Background: Dental caries, a globally prevalent disease, burdens health and economies. Targeting Candida albicans-Streptococcus mutans synergism aids managing severe early childhood caries and root caries. Artemisinin, a natural antimalarial, inhibits C. albicans virulence via hyphal and biofilm suppression, promising to disrupt this cross-kingdom cariogenic partnership. Objective: This study evaluated the inhibitory effects of seven common artemisinin compounds on the synergistic cariogenic effect of C. albicans-S. mutans biofilm, in order to expand the application of artemisinin compounds in the field of caries prevention. Methods: Hyphal induction, biofilm formation, and CFU assays evaluated the inhibitory efficacy of the compounds against C. albicans and S. mutans. RT-qPCR examinations analyzed the expression of key virulence genes in both species. The anti-caries efficacy of artemisinin compounds was further validated in a rat caries model, demonstrated by quantitative analysis of microbial load in dental plaque and Keyes scoring for molar carious lesions. Results: All seven artemisinin derivatives effectively inhibited hyphal formation in C. albicans. Artemisinic acid and dihydroartemisinic acid additionally exhibited direct antibacterial activity against S. mutans, significantly suppressing dual-species biofilm formation, EPS synthesis, and acid production. All compounds downregulated key virulence genes in both pathogens. In vivo studies confirmed that artemisinic acid and dihydroartemisinic acid reduced microbial colonization and caries severity, while other derivatives primarily exerted anticaries effects by inhibiting C. albicans colonization. Conclusion: Artemisinin compounds can reduce the synergistic cariogenic virulence of C. albicans and S. mutans by inhibiting the interaction between C. albicans and S. mutans. (Copyright © 2026 Zhou, Zhou, Shen, Lin, Lei, Gou, Meng, Zhou, Liao, Cheng, Zhou and Ren.) |
| Competing Interests: | The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The reviewer ZH declared a past co-authorship with the authors LC, XZ to the handling editor. |
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| Contributed Indexing: | Keywords: Candida albicans; Streptococcus mutans; artemisinin compounds; dental caries; synergistic cariogenic |
| Substance Nomenclature: | 0 (Artemisinins) 0 (Anti-Bacterial Agents) |
| Entry Date(s): | Date Created: 20260923 Date Completed: 20260923 Latest Revision: 20260924 |
| Update Code: | 20260924 |
| PubMed Central ID: | PMC13593465 |
| DOI: | 10.3389/fcimb.2026.1950360 |
| PMID: | 42774055 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 2235-2988 |
|---|---|
| DOI: | 10.3389/fcimb.2026.1950360 |