Academic Journal
Live Imaging of Ions in Cells Infected with Chlamydia trachomatis.
| Τίτλος: | Live Imaging of Ions in Cells Infected with Chlamydia trachomatis. |
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| Συγγραφείς: | Weild R; Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QP, UK., Hayward RD; Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QP, UK. rdh24@cam.ac.uk. |
| Πηγή: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2027; Vol. 3073, pp. 81-93. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE |
| Imprint Name(s): | Publication: Totowa, NJ : Humana Press Original Publication: Clifton, N.J. : Humana Press |
| Ιατρικοί όροι (MeSH): | Chlamydia trachomatis*/metabolism , Chlamydia trachomatis*/physiology , Chlamydia Infections*/metabolism , Chlamydia Infections*/microbiology , Potassium*/metabolism , Ions*/metabolism, Microscopy, Confocal/methods ; Time-Lapse Imaging/methods ; Humans ; HeLa Cells ; Host-Pathogen Interactions ; Ion Transport |
| Περίληψη: | This chapter describes methodological approaches for studying intracellular ion dynamics during Chlamydia trachomatis infection. It emphasizes the requirement for live-cell imaging to capture active and compartmentalized ion fluxes in infected cells precluded by fixed-cell techniques. Specifically, we present step-by-step protocols for labeling Chlamydia-infected cells with a fluorescent potassium ion (K+)-sensitive probe for performing live imaging with confocal microscopy or spinning disc confocal microscopy for higher resolution and procedures for image processing and quantification. We also describe time-lapse microscopy to monitor K+ dynamics over the extended time course of the infection. These advanced microscopy methods have the potential to visualize ion transport in real time at the host-pathogen interface. (© 2027. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.) |
| Competing Interests: | Competing Interests: The authors have no conflicts of interest to declare that are relevant to the content of this chapter. |
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| Contributed Indexing: | Keywords: Chlamydia; Confocal microscopy; Ion; Live imaging; Probe; Super-resolution |
| Substance Nomenclature: | RWP5GA015D (Potassium) 0 (Ions) |
| Entry Date(s): | Date Created: 20260922 Date Completed: 20260922 Latest Revision: 20260922 |
| Update Code: | 20260923 |
| DOI: | 10.1007/978-1-0716-5531-3_6 |
| PMID: | 42771143 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1940-6029 |
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| DOI: | 10.1007/978-1-0716-5531-3_6 |