Academic Journal
Evaluation the role of quinoa seeds in attenuation the brain cellular senescence and aging induced by D-galactose and γ-radiation in rats: insights into autophagy, telomerase activity, amyloid-β and tau proteins.
| Τίτλος: | Evaluation the role of quinoa seeds in attenuation the brain cellular senescence and aging induced by D-galactose and γ-radiation in rats: insights into autophagy, telomerase activity, amyloid-β and tau proteins. |
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| Συγγραφείς: | Mohamed MA; Biochemistry Department, Faculty of Science, Ain Shams University, Cairo, Egypt. merehanfahem@sci.asu.edu.eg., Elbakry MMM; Biochemistry Department, Faculty of Science, Ain Shams University, Cairo, Egypt.; Faculty of Biotechnology, October University for Modern Sciences and Arts (MSA), Cairo, Egypt., Elbakary NM; Radiation Biology Department, National Centre for Radiation Research & Technology, Egyptian Atomic-Energy Authority, Cairo, Egypt., Moselhy SS; Biochemistry Department, Faculty of Science, Ain Shams University, Cairo, Egypt., Said YA; Biochemistry Department, Faculty of Science, Ain Shams University, Cairo, Egypt. |
| Πηγή: | Inflammopharmacology [Inflammopharmacology] 2026 Oct; Vol. 34 (10), pp. 6819-6838. Date of Electronic Publication: 2026 Sep 19. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Birkhäuser Country of Publication: Switzerland NLM ID: 9112626 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1568-5608 (Electronic) Linking ISSN: 09254692 NLM ISO Abbreviation: Inflammopharmacology Subsets: MEDLINE |
| Imprint Name(s): | Publication: Basel ; Boston : Birkhäuser Original Publication: Dordrecht, The Netherlands ; Norwell, MA, USA : Kluwer Academic Publishers, c1991- |
| Ιατρικοί όροι (MeSH): | Brain*/drug effects , Brain*/metabolism , Seeds*/chemistry , Aging*/drug effects , Aging*/metabolism , Chenopodium quinoa*/chemistry , Cellular Senescence*/drug effects , Cellular Senescence*/radiation effects , Plant Extracts*/pharmacology, Autophagy/drug effects ; Galactose/toxicity ; Telomerase/metabolism ; Amyloid beta-Peptides/metabolism ; Oxidative Stress/drug effects ; tau Proteins/metabolism ; Gamma Rays/adverse effects ; Antioxidants/pharmacology ; Animals ; Male ; Rats ; Molecular Docking Simulation |
| Περίληψη: | The gradual loss of cognitive function, manifested by impaired learning and spatial memory, is a hallmark of brain senescence during aging. However, effective natural interventions targeting multiple mechanisms involved in aging-related neurodegeneration remain limited. This study assessed the possible protective efficacy of quinoa seed powder (QSP) against D-galactose (D-gal)- and γ-radiation-induced brain senescence in rats. Fifty male albino rats (n = 10/group) were randomly allocated into five experimental groups. Aging was induced by D-gal administration and fractionated whole-body γ-radiation (1.5 Gy/week for four consecutive weeks; total dose 6 Gy). Behavioral performance, oxidative stress biomarkers, telomerase activity, autophagy markers, neurodegenerative proteins, histopathological alterations, antioxidant activity of quinoa ethanolic extract (QEE), and molecular docking were evaluated. QSP significantly (P < 0.05) attenuated oxidative stress, as evidenced by decreased malondialdehyde levels and increased catalase activity and reduced glutathione content. Furthermore, QSP significantly reduced amyloid-β 1-42 (Aβ42) and Tau protein accumulation, increased telomerase activity, and enhanced autophagy-related modulation through upregulation of microtubule-associated protein 1 light chain 3 beta (LC3B) and downregulation of mammalian target of rapamycin (mTOR) expression. In vitro analysis showed that QEE exhibited potent antioxidant activity, with EC50 values of 16.67 and 23.01 mg/mL for TAC and FRAP, respectively, and an IC (© 2026. The Author(s).) |
| Competing Interests: | Declarations. Competing interests: The authors declare that they have no competing interests. Ethics approval: The study was reviewed and approved by the Research Ethics Committee, Faculty of Science, Ain Shams University (Approval No. ASU-SCI/BIOC/2025/6/2; reviewed on 15 June 2025). The study was conducted in accordance with the ARRIVE 2.0 guidelines and relevant regulations governing animal experimentation. |
| References: | Abugoch LE, Romero N, Tapia CA, Silva J, Rivera M (2008) Study of some physicochemical and functional properties of quinoa (Chenopodium quinoa Willd) protein isolates. J Agric Food Chem 56:4745–4750. (PMID: 1848911910.1021/jf703689u) Ahmed D, Baig H, Zara S (2012) Seasonal variation of phenolics, flavonoids, antioxidant and lipid peroxidation inhibitory activity of methanolic extract of Melilotus indicus and its sub-fractions in different solvents. Int J Phytomed 4:326–332. Almeida AJPOD, Ribeiro TP, Medeiros IAD (2017) Aging: molecular pathways and implications on the cardiovascular system. Oxidative Med Cell Longev 2017:7941563. (PMID: 10.1155/2017/7941563) Amick KA, Mahapatra G, Gao Z, Dewitt A, Craft S, Jain M, Molina AJ (2022) Plasma glycocholic acid and linoleic acid identified as potential mediators of mitochondrial bioenergetics in Alzheimer’s dementia. Front Aging Neurosci 14:954090. (PMID: 36212044954036410.3389/fnagi.2022.954090) Aniess WI, Gohari ST, Goma AA, Ahmad WA (2020) Protective effect of the red quinoa seeds versus oxidative stress induced by alloxan and carbon tetrachloride in experimental rats. Bull Natl Nutr Inst Arab Repub Egypt 55:1–38. (PMID: 10.21608/bnni.2020.162860) Arshad M, Kousar S, Din A, Afzaal M, Faisal MN, Sharif MK, Rasheed H, Saeed F, Akram N, Ahmed F (2024) Hepatoprotective efficacy of quinoa seed extract against CCl4-induced acute liver toxicity in rat model. Food Sci Nutr 12:5007–5018. (PMID: 390551881126692210.1002/fsn3.4149) Avila J, Hernández F, Viña J (2025) Tau protein isoforms in neuropathological aging: gerosuppressors, gerogenes or just travel companions. Geromedicine 1:202503–202503. Bayraktaroğlu SB, Turan RD, Taşlı NP, Şahin F (2025) Evaluation of anti-aging agents using the d-galactose-induced accelerated aging model. Stem cells and aging: methods and protocols. Springer. Blois MS (1958) Antioxidant determinations by the use of a stable free radical. Nature 181:1199–1200. (PMID: 10.1038/1811199a0) Cai W, Li L, Sang S, Pan X, Zhong C (2023) Physiological roles of β-amyloid in regulating synaptic function: implications for AD pathophysiology. Neurosci Bull 39:1289–1308. (PMID: 10.1007/s12264-022-00985-936443453) Calderon-Montano M, Burgos-Moron J, Pérez-Guerrero E, Lopez-Lazaro C (2011) A review on the dietary flavonoid kaempferol. Mini Rev Med Chem 11:298–344. (PMID: 2142890110.2174/138955711795305335) Chen X, Li Y, Chen W, Nong Z, Huang J, Chen C (2016) Protective effect of hyperbaric oxygen on cognitive impairment induced by D-galactose in mice. Neurochem Res 41:3032–3041. (PMID: 10.1007/s11064-016-2022-x27485714) Chou S-M, Yen Y-H, Yuan F, Zhang S-C, Chong C-M (2023) Neuronal senescence in the aged brain. Aging Dis 14:1618. (PMID: 371961171052974410.14336/AD.2023.0214) D’angelo S (2023) Diet and aging: the role of polyphenol-rich diets in slow down the shortening of telomeres: a review. Antioxidants 12:2086. (PMID: 381362061074076410.3390/antiox12122086) Drury R (1983) Theory and practice of histological techniques. J Clin Pathol 36:609. (PMID: 49831310.1136/jcp.36.5.609-d) El Hazzam K, Hafsa J, Sobeh M, Mhada M, Taourirte M, El Kacimi K, Yasri A (2020) An insight into saponins from Quinoa (Chenopodium quinoa Willd): a review. Molecules 25:1059. (PMID: 32120971717910810.3390/molecules25051059) Freyssin A, Page G, Fauconneau B, Bilan AR (2018) Natural polyphenols effects on protein aggregates in Alzheimer’s and Parkinson’s prion-like diseases. Neural Regen Res 13:955. (PMID: 29926816602247910.4103/1673-5374.233432) Gao Q, Fan M, Qian H, Li Y, Wang L (2025) Quinoa polyphenols alleviate glucose deprivation-induced endoplasmic reticulum stress by enhancing hepatic cellular autophagy and antioxidant capacity. Mol Nutr Food Res 69:e70279. (PMID: 4101413610.1002/mnfr.70279) Glynn C, Rodriguez JA, Hyman BT (2024) The structural line between prion and “prion-like”: insights from prion protein and tau. Curr Opin Neurobiol. https://doi.org/10.1016/j.conb.2024.102857. (PMID: 10.1016/j.conb.2024.1028573848986511162956) Han Y-L, Yin H-H, Xiao C, Bernards MT, He Y, Guan Y-X (2023) Understanding the molecular mechanisms of polyphenol inhibition of amyloid β aggregation. ACS Chem Neurosci 14:4051–4061. (PMID: 3789013110.1021/acschemneuro.3c00586) Han S-H, Lee J-H, Woo J-S, Jung G-H, Jung S-H, Han E-J, Park Y-S, Kim B-S, Kim S-K, Park B-K (2024) Platycodin D induces apoptosis via regulating MAPK pathway and promotes autophagy in colon cancer cell. Biomed Pharmacother 172:116216. (PMID: 3829575510.1016/j.biopha.2024.116216) Herbert B-S, Hochreiter AE, Wright WE, Shay JW (2006) Nonradioactive detection of telomerase activity using the telomeric repeat amplification protocol. Nat Protoc 1:1583–1590. (PMID: 10.1038/nprot.2006.23917406450) Hole KL, Williams RJ (2020) Flavonoids as an intervention for Alzheimer’s disease: progress and hurdles towards defining a mechanism of action. Brain Plast 6:167–192. (PMID: 10.3233/BPL-200098) Hruby AJ, Higuchi-Sanabria R (2025) Mitochondrial dysfunction in cellular senescence: a bridge to neurodegenerative disease. Npj Aging 11:99. (PMID: 10.1038/s41514-025-00291-44140233912708852) Hussain MI, Farooq M, Syed QA, Ishaq A, Al-Ghamdi AA, Hatamleh AA (2021) Botany, nutritional value, phytochemical composition and biological activities of quinoa. Plants 10:2258. (PMID: 34834624862408510.3390/plants10112258) Ibragimova M, Kussainova A, Aripova A, Bersimbaev R, Bulgakova O (2024) The molecular mechanisms in senescent cells induced by natural aging and ionizing radiation. Cells 13:550. (PMID: 385343941096941610.3390/cells13060550) Janet Olufunmilayo O, Olugbenga Ayodeji A, James Oluwabusayo A (2025) Neurobehavioural changes and oxidative damage due to aluminium chloride-induced neurotoxicity in adult female wistar rats: Phoenix Dactylifera L. fruit to the rescue. Brain Sci Adv 11:9050012. (PMID: 10.26599/BSA.2024.9050012) Jin M, Cai S-Q (2023) Mechanisms underlying brain aging under normal and pathological conditions. Neurosci Bull 39:303–314. (PMID: 10.1007/s12264-022-00969-936437436) Kobayashi S, Tanaka T, Soeda Y, Takashima A (2019) Enhanced tau protein translation by hyper-excitation. Front Aging Neurosci 11:322. (PMID: 31824301687955410.3389/fnagi.2019.00322) Koumayha A, Borjac J, Matar A (2026) The effect of Chenopodium quinoa Willd. Extract on DMH-induced colorectal cancer and hepatic oxidative stress through monitoring PI3K/Akt/mTOR pathway. Adv Pharmacol Pharmaceut Sci 202:5552606. Lan Y, Song Y, Zhang W, Zhao S, Wang X, Wang L, Wang Y, Yang X, Wu H, Liu X (2025a) Quinoa ethanol extract ameliorates cognitive impairments induced by hypoxia in mice: insights into antioxidant defense and gut microbiome modulation. J Agric Food Chem 73:3427–3443. (PMID: 3987345510.1021/acs.jafc.4c07530) Lan Y, Zhao S, Song Y, Zhang W, Hao J, Wang X, Zhu Z, Ding L, Wang Y, Liu X (2025b) Physicochemical properties of selenized quinoa protein hydrolysate and its regulatory effects on neuroinflammation and gut microbiota in hypoxic mice. J Future Foods. https://doi.org/10.1016/j.jfutfo.2025.09.044. (PMID: 10.1016/j.jfutfo.2025.09.044) Li L, Lietz G, Bal W, Watson A, Morfey B, Seal C (2018) Effects of quinoa (Chenopodium quinoa Willd.) consumption on markers of CVD risk. Nutrients 10:777. (PMID: 29914146602432310.3390/nu10060777) Li L, Lietz G, Seal CJ (2024) Effects of quinoa intake on markers of cardiovascular risk: a systematic literature review and meta-analysis. Food Rev Int 40:1–19. (PMID: 10.1080/87559129.2022.2148689) Ma W, Wei S, Peng W, Sun T, Huang J, Yu R, Zhang B, Li W (2021) Antioxidant effect of Polygonatum sibiricum polysaccharides in D-galactose-induced heart aging mice. Biomed Res Int 2021:6688855. (PMID: 33860051802408610.1155/2021/6688855) Ma Y, Wang X, Li X, Chen X, Teng Z, Wang X, Yang J, Liu G (2024) COP-22 alleviates d-galactose–induced brain aging by attenuating oxidative stress, inflammation, and apoptosis in mice. Mol Neurobiol 61:6708–6720. (PMID: 10.1007/s12035-024-03976-13834728511339142) Mannick JB, Lamming DW (2023) Targeting the biology of aging with mTOR inhibitors. Nat Aging 3:642–660. (PMID: 10.1038/s43587-023-00416-y3714283010330278) Martinovic J, Kontic MZ, Dragic M, Todorovic A, Stojanovic IG, Mitrovic N, Grkovic I, Drakulic D (2023) Chronic oral d-galactose intake provokes age-related changes in the rat prefrontal cortex. Behav Brain Res 436:114072. (PMID: 3603090610.1016/j.bbr.2022.114072) Morris GM, Huey R, Lindstrom W, Sanner MF, Belew RK, Goodsell DS, Olson AJ (2009) AutoDock4 and AutoDockTools4: automated docking with selective receptor flexibility. J Comput Chem 30:2785–2791. (PMID: 19399780276063810.1002/jcc.21256) Mu H, Xue S, Sun Q, Shi J, Zhang D, Wang D, Wei J (2023) Research progress of quinoa seeds (Chenopodium quinoa Wild.): nutritional components, technological treatment, and application. Foods 12:2087. (PMID: 372389051021762210.3390/foods12102087) Ng CY, Wang M (2021) The functional ingredients of quinoa (Chenopodium quinoa) and physiological effects of consuming quinoa: a review. Food Front 2:329–356. (PMID: 10.1002/fft2.109) Noureen S, Riaz A, Arshad M, Arshad N (2019) In vitro selection and in vivo confirmation of the antioxidant ability of Lactobacillus brevis MG000874. J Appl Microbiol 126:1221–1232. (PMID: 3059772610.1111/jam.14189) Nsimba RY, Kikuzaki H, Konishi Y (2008) Antioxidant activity of various extracts and fractions of Chenopodium quinoa and Amaranthus spp. seeds. Food Chem 106:760–766. (PMID: 10.1016/j.foodchem.2007.06.004) O’boyle NM, Banck M, James CA, Morley C, Vandermeersch T, Hutchison GR (2011) Open babel: an open chemical toolbox. J Cheminform 3:33. (PMID: 10.1186/1758-2946-3-33219823003198950) Oyaizu M (1986) Studies on products of browning reaction antioxidative activities of products of browning reaction prepared from glucosamine. Jpn J Nutrit Dietetics 44:307–315. (PMID: 10.5264/eiyogakuzashi.44.307) Pantiya P, Thonusin C, Chunchai T, Pintana H, Ongnok B, Nawara W, Arunsak B, Kongkaew A, Chattipakorn N, Chattipakorn SC (2023a) Long-term lifestyle intervention is superior to transient modification for neuroprotection in D-galactose-induced aging rats. Life Sci 334:122248. (PMID: 3794006910.1016/j.lfs.2023.122248) Pantiya P, Thonusin C, Ongnok B, Chunchai T, Kongkaew A, Nawara W, Arunsak B, Chattipakorn N, Chattipakorn SC (2023b) Long-term D-galactose administration mimics natural aging in rat’s hippocampus. Alzheimers Dement 19:e073540. (PMID: 10.1002/alz.073540) Park JH, Lee YJ, Kim YH, Yoon KS (2017) Antioxidant and antimicrobial activities of quinoa (Chenopodium quinoa Willd.) seeds cultivated in Korea. Prevent Nutrit Food Sci 22:195. Rehman SU, Shah SA, Ali T, Chung JI, Kim MO (2017) Anthocyanins reversed D-galactose-induced oxidative stress and neuroinflammation mediated cognitive impairment in adult rats. Mol Neurobiol 54:255–271. (PMID: 10.1007/s12035-015-9604-526738855) Rejili M, Al-Kuraishy HM, Shokr MM, Batiha GE-S (2026) Unlocking the aging brain: mTORC1 as a convergent integrator for neurodegeneration and therapeutic intervention. Biogerontology 27:115. (PMID: 10.1007/s10522-026-10457-642295556) Rossiello F, Jurk D, Passos JF, D’adda Di Fagagna F (2022) Telomere dysfunction in ageing and age-related diseases. Nat Cell Biol 24:135–147. (PMID: 10.1038/s41556-022-00842-x351654208985209) Salama A, El Shahed ZAE (2024) Naringin attenuates D-galactose-induced brain aging via regulation of oxidative stress markers TNF-α, NF-κβ and modulation of the neurotrophic markers PGC1-α, NT-3 AGEs, and GFAP in vivo. Egypt J Chem 67:1–15. Sedik AA, El-Mosallamy AE, Yassen NN, El-Nasr NMA (2025) Dose dependent toxicity of streptozotocin inducing diabetic complications in male Sprague Dawley rats with metabolic biochemical histopathological effects. Sci Rep 15:43381. (PMID: 10.1038/s41598-025-27100-y4136121312689764) Shahzad A, Faisal MN, Hussain G, Naqvi SAR, Anwar H (2021) Therapeutic potential of quinoa seed extract as regenerative and hepatoprotective agent in induced liver injury wistar rat model. Pak J Pharmaceut Sci 34:2309–2315. Shang H, Sun J, Zheng Z, Sun S, Yan X (2025) Study on the effect of quinoa saponins on human colon cancer HT-29 cells. Food Sci Nutr 13:e4669. (PMID: 3980323310.1002/fsn3.4669) Solo P (2021) Potential inhibitors of SARS-CoV-2 (COVID 19) spike protein of the delta and delta plus variant: in silico studies of medicinal plants of North-East India. Curr Res Pharmacol Drug Discov 2:100065. (PMID: 34870160853077810.1016/j.crphar.2021.100065) Souza SP, Roos AA, Gindri AL, Domingues VO, Ascari J, Guerra GP (2020) Neuroprotective effect of red quinoa seeds extract on scopolamine-induced declarative memory deficits in mice: the role of acetylcholinesterase and oxidative stress. J Funct Foods 69:103958. (PMID: 10.1016/j.jff.2020.103958) Terreros G, Pérez MÁ, Muñoz-Llancao P, D’espessailles A, Martínez EA, Dagnino-Subiabre A (2024) The neuroprotective role of quinoa (Chenopodium quinoa, Wild) supplementation in hippocampal morphology and memory of adolescent stressed rats. Nutrients 16:381. (PMID: 383376651085738010.3390/nu16030381) Vilcacundo R, Hernández-Ledesma B (2017) Nutritional and biological value of quinoa (Chenopodium quinoa Willd.). Curr Opin Food Sci 14:1–6. (PMID: 10.1016/j.cofs.2016.11.007) Viña J, Borrás C, Mas-Bargues C (2024) Free radicals in Alzheimer’s disease: from pathophysiology to clinical trial results. Free Radic Biol Med 225:296–301. (PMID: 3937005510.1016/j.freeradbiomed.2024.09.051) Vossel KA, Xu JC, Fomenko V, Miyamoto T, Suberbielle E, Knox JA, Ho K, Kim DH, Yu G-Q, Mucke L (2015) Tau reduction prevents Aβ-induced axonal transport deficits by blocking activation of GSK3β. J Cell Biol 209:419–433. (PMID: 25963821442778910.1083/jcb.201407065) Wei P, Zhang X, Yan C, Sun S, Chen Z, Lin F (2025) Mitochondrial dysfunction and aging: multidimensional mechanisms and therapeutic strategies. Biogerontology 26:142. (PMID: 10.1007/s10522-025-10273-44063482512241157) Xi X, Fan G, Xue H, Peng S, Huang W, Zhan J (2024) Harnessing the potential of quinoa: nutritional profiling, bioactive components, and implications for health promotion. Antioxidants (Basel) 13:829. (PMID: 390618981127395010.3390/antiox13070829) Xu X, Pang Y, Fan X (2025) Mitochondria in oxidative stress, inflammation and aging: from mechanisms to therapeutic advances. Signal Transduct Target Ther 10:190. (PMID: 405002581215921310.1038/s41392-025-02253-4) Yakunchikova E, Yurova M, Drachyov I, Radetskaya E, Altukhov K, Semenov A, Panchenko A, Tyndyk M, Bykov V, Fedoros E (2024) Model of accelerated aging in CB6F2 mice induced by ionizing radiation. Bull Exp Biol Med 177:363–367. (PMID: 10.1007/s10517-024-06190-039126549) Younis IY, Sedeek MS, Essa AF, Elgamal AM, Eltanany BM, Goda ZM, Pont L, Benavente F, Mohsen E (2025) Exploring geographic variations in Quinoa grains: unveiling anti-Alzheimer activity via GC–MS, LC-QTOF-MS/MS, molecular networking, and chemometric analysis. Food Chem 465:141918. (PMID: 3954169110.1016/j.foodchem.2024.141918) Zhang L, Zhang J, Zang H, Yin Z, Guan P, Yu C, Shan A, Feng X (2024) Dietary pterostilbene exerts potential protective effects by regulating lipid metabolism and enhancing antioxidant capacity on liver in broilers. J Anim Physiol Anim Nutr 108:921–933. (PMID: 10.1111/jpn.13941) Zhou Z, Zhang P, Li J, Yao J, Jiang Y, Wan M, Tang W, Liu L (2024) Autophagy and the pancreas: healthy and disease states. Front Cell Dev Biol 12:1460616. (PMID: 393813721145838910.3389/fcell.2024.1460616) Zhu J, Mu X, Zeng J, Xu C, Liu J, Zhang M, Li C, Chen J, Li T, Wang Y (2014) Ginsenoside Rg1 prevents cognitive impairment and hippocampus senescence in a rat model of D-galactose-induced aging. PLoS ONE 9:e101291. (PMID: 24979747407629610.1371/journal.pone.0101291) Zhu W, Zhang X, Yu M, Lin B, Yu C (2021) Radiation-induced liver injury and hepatocyte senescence. Cell Death Discov 7:244. (PMID: 10.1038/s41420-021-00634-6345313768446062) |
| Contributed Indexing: | Keywords: Aging; Autophagy; Brain cellular senescence; Oxidative stress; Quinoa seeds; Rats; Telomerase |
| Substance Nomenclature: | X2RN3Q8DNE (Galactose) EC 2.7.7.49 (Telomerase) 0 (Amyloid beta-Peptides) 0 (tau Proteins) 0 (Plant Extracts) 0 (Antioxidants) |
| Entry Date(s): | Date Created: 20260919 Date Completed: 20261017 Latest Revision: 20261017 |
| Update Code: | 20261017 |
| DOI: | 10.1007/s10787-026-02377-1 |
| PMID: | 42762380 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1568-5608 |
|---|---|
| DOI: | 10.1007/s10787-026-02377-1 |