Academic Journal
Linking cytokine signaling to vascular response: Impedance biosensing of E-selectin and IL-6 for endothelial activation assessment.
| Τίτλος: | Linking cytokine signaling to vascular response: Impedance biosensing of E-selectin and IL-6 for endothelial activation assessment. |
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| Συγγραφείς: | David BE; Department of Bioengineering, The University of Texas at Dallas, Richardson, TX, 75248, USA., Madhurantakam S; Department of Bioengineering, The University of Texas at Dallas, Richardson, TX, 75248, USA., Mathew G; Department of Bioengineering, The University of Texas at Dallas, Richardson, TX, 75248, USA., Desai AM; Department of Bioengineering, The University of Texas at Dallas, Richardson, TX, 75248, USA., Karnam JB; EnLiSense LLC, Allen, TX, 75013, USA., Dhamu VN; EnLiSense LLC, Allen, TX, 75013, USA., Muthukumar S; EnLiSense LLC, Allen, TX, 75013, USA., Prasad S; Department of Bioengineering, The University of Texas at Dallas, Richardson, TX, 75248, USA. Electronic address: Shalini.Prasad@UTDallas.edu. |
| Πηγή: | Biosensors & bioelectronics [Biosens Bioelectron] 2027 Jan 01; Vol. 315, pp. 119230. Date of Electronic Publication: 2026 Sep 17. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Elsevier Advanced Technology Country of Publication: England NLM ID: 9001289 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-4235 (Electronic) Linking ISSN: 09565663 NLM ISO Abbreviation: Biosens Bioelectron Subsets: MEDLINE |
| Imprint Name(s): | Publication: Oxford : Elsevier Advanced Technology Original Publication: [Barking, Essex, England] : Elsevier Applied Science, 1989- |
| Ιατρικοί όροι (MeSH): | E-Selectin*/blood , Interleukin-6*/blood , Dielectric Spectroscopy*/instrumentation , Dielectric Spectroscopy*/methods , Biosensing Techniques*/instrumentation, Gold/chemistry ; Biomarkers/blood ; Humans ; Equipment Design ; Limit of Detection |
| Περίληψη: | Endothelial activation is a key feature of vascular inflammation, yet rapid methods for simultaneously quantifying inflammatory cytokines and endothelial activation biomarkers remain limited. Here, we present a portable electrochemical impedance spectroscopy (EIS) biosensing platform for the label-free detection of interleukin-6 (IL-6) and E-selectin directly in human plasma. The platform utilizes ZnO-modified gold electrodes to support antibody immobilization and enhance impedance-based signal transduction, enabling rapid biomarker quantification using pre-functionalized disposable sensors. Sensor performance was characterized through surface functionalization, electrochemical impedance analysis, and equivalent circuit modeling, followed by analytical validation using calibration, spike-and-recovery, specificity, and precision studies. The biosensor demonstrated concentration-dependent impedance responses across the evaluated measurement ranges, with regression-based limits of detection of 55.77 pg/mL for IL-6 and 3.38 ng/mL for E-selectin. Analytical validation demonstrated excellent quantitative performance, including calibration coefficients of determination up to 0.98, spike-and-recovery values within 80-120%, minimal cross-reactivity with representative non-target proteins, and intra- and inter-assay coefficients of variation below 20%. Measurements were completed using less than 100 μL of plasma with a total assay time of under 10 min. The portable READ platform demonstrated analytical performance comparable to a benchtop impedance analyzer, supporting reliable quantitative biomarker detection in plasma. Collectively, this work establishes a portable multiplex electrochemical biosensing strategy for rapid inflammatory profiling and provides a foundation for future point-of-care monitoring of endothelial dysfunction and vascular inflammation. (Copyright © 2026 Elsevier B.V. All rights reserved.) |
| Competing Interests: | Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. |
| Contributed Indexing: | Keywords: E-selectin; Electrochemical impedance spectroscopy; Endothelial dysfunction; IL-6; Label-free biosensor; Point-of-care diagnostics |
| Substance Nomenclature: | 0 (E-Selectin) 0 (Interleukin-6) 0 (IL6 protein, human) 7440-57-5 (Gold) 0 (Biomarkers) |
| Entry Date(s): | Date Created: 20260918 Date Completed: 20261010 Latest Revision: 20261010 |
| Update Code: | 20261011 |
| DOI: | 10.1016/j.bios.2026.119230 |
| PMID: | 42759301 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1873-4235 |
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| DOI: | 10.1016/j.bios.2026.119230 |