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Local anesthetic lidocaine decelerates cytoplasmic streaming in Egeria densa via altered actin dynamics and reactive oxygen species generation.

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Τίτλος: Local anesthetic lidocaine decelerates cytoplasmic streaming in Egeria densa via altered actin dynamics and reactive oxygen species generation.
Συγγραφείς: Magari N; Department of Anesthesiology, Keio University School of Medicine, Shinjuku, Tokyo, Japan.; Faculty of Engineering, Kitami Institute of Technology, Kitami, Hokkaido, Japan., Kagenishi T; Faculty of Engineering, Kitami Institute of Technology, Kitami, Hokkaido, Japan., Yokawa K; Faculty of Engineering, Kitami Institute of Technology, Kitami, Hokkaido, Japan.
Πηγή: Plant signaling & behavior [Plant Signal Behav] 2026 Dec 31; Vol. 21 (1), pp. 2726830. Date of Electronic Publication: 2026 Sep 16.
Τύπος έκδοσης: Journal Article
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: Taylor & Francis Country of Publication: United States NLM ID: 101291431 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1559-2324 (Electronic) Linking ISSN: 15592316 NLM ISO Abbreviation: Plant Signal Behav Subsets: MEDLINE
Imprint Name(s): Publication: 2015- : Philadelphia, PA : Taylor & Francis
Original Publication: Georgetown, TX : Landes Bioscience
Ιατρικοί όροι (MeSH): Lidocaine*/pharmacology , Reactive Oxygen Species*/metabolism , Anesthetics, Local*/pharmacology , Cytoplasmic Streaming*/drug effects , Actins*/metabolism , Hydrocharitaceae*/drug effects , Hydrocharitaceae*/metabolism, Calcium/metabolism ; Cytoplasm/metabolism ; Cytoplasm/drug effects ; NADPH Oxidases/metabolism
Περίληψη: Local anesthetics (LAs) block voltage-gated Na+ channels in mammals, but they also affect cytoplasmic streaming in plant cells. Although certain LAs inhibit streaming in algae such as Chara, the underlying mechanisms remain unclear. This study aimed to elucidate how LAs reduce cytoplasmic streaming velocity, focusing on the actomyosin system and oxidative stress responses, using lidocaine as a representative LA. Streaming velocity in Egeria densa leaves decreased reversibly after lidocaine treatment and recovered following a washout procedure. Confocal laser scanning microscopy of fluorescently stained actin filaments revealed that lidocaine disrupted F-actin structures. Electron spin resonance analysis showed that lidocaine induced reactive oxygen species (ROS) production in a dose-dependent manner, and spatiotemporal fluorescence analysis with ROS-sensitive probes demonstrated that ROS accumulated near the plasma membrane and increased over time; this process appeared to be mediated by nicotinamide adenine dinucleotide phosphate (NADPH) oxidases and Ca2+ influx. Application of antioxidants attenuated the lidocaine-induced reduction in streaming velocity. In conclusion, lidocaine slows cytoplasmic streaming in E. densa by eliciting a reversible, multifaceted response involving NADPH oxidase-derived ROS, Ca2+ influx, and actomyosin remodeling, offering mechanistic insight into the still poorly understood phenomenon of local anesthetic action in plants.
References: Plant Cell Rep. 2024 Nov 24;43(12):293. (PMID: 39580775)
Adv Biol (Weinh). 2025 Dec;9(12):e00486. (PMID: 41194452)
J Cell Biol. 1976 Feb;68(2):395-402. (PMID: 1245553)
J Cell Physiol. 1968 Dec;72(3):153-60. (PMID: 5724566)
Plant Signal Behav. 2024 Dec 31;19(1):2388443. (PMID: 39116108)
Plant Signal Behav. 2024 Dec 31;19(1):2339574. (PMID: 38601988)
Plant Signal Behav. 2022 Dec 31;17(1):2106410. (PMID: 35938584)
Mitochondrion. 2010 Aug;10(5):487-96. (PMID: 20546950)
Sci Signal. 2009 Aug 18;2(84):ra45. (PMID: 19690331)
J Am Chem Soc. 2013 Apr 10;135(14):5389-98. (PMID: 23484901)
Eur Biophys J. 2005 Nov;34(8):973-86. (PMID: 15971063)
Planta. 1991 Mar;183(4):567-74. (PMID: 24193850)
J Exp Bot. 2003 Jan;54(380):103-13. (PMID: 12456760)
Curr Opin Cell Biol. 2004 Feb;16(1):68-72. (PMID: 15037307)
Ann Bot. 2018 Nov 03;122(5):747-756. (PMID: 29236942)
Anesthesiology. 1990 Apr;72(4):711-34. (PMID: 2157353)
Nat Struct Mol Biol. 2025 Feb;32(2):237-242. (PMID: 39448849)
Photosynth Res. 1989 Nov;22(2):137-46. (PMID: 24424746)
Dev Cell. 2013 Nov 11;27(3):345-52. (PMID: 24229646)
J Biol Chem. 2012 Aug 31;287(36):30711-8. (PMID: 22740687)
Lancet. 1968 Dec 21;2(7582):1326-9. (PMID: 4177393)
Anesth Prog. 2012 Summer;59(2):90-101; quiz 102-3. (PMID: 22822998)
Plant Physiol. 2000 Jun;123(2):645-54. (PMID: 10859194)
Free Radic Biol Med. 1987;3(4):259-303. (PMID: 2826304)
Anesthesiology. 2007 Jul;107(1):136-43. (PMID: 17585225)
Plant Cell. 1998 Feb;10(2):255-66. (PMID: 9490748)
J Biol Chem. 2008 Apr 4;283(14):8885-92. (PMID: 18218618)
Front Plant Sci. 2020 Jun 30;11:968. (PMID: 32695131)
Korean J Physiol Pharmacol. 2012 Dec;16(6):413-22. (PMID: 23269904)
Proc Natl Acad Sci U S A. 1974 Nov;71(11):4457-61. (PMID: 4530994)
Free Radic Biol Med. 2018 Jul;122:21-27. (PMID: 29203327)
Sci Adv. 2025 Dec 5;11(49):eadw6915. (PMID: 41337577)
Nature. 1982 Apr 15;296(5858):647-50. (PMID: 7070508)
Nat Plants. 2015 Mar 23;1(4):15031. (PMID: 27247032)
Curr Opin Plant Biol. 2015 Oct;27:104-10. (PMID: 26202096)
Plant J. 2005 Oct;44(2):342-7. (PMID: 16212611)
Contributed Indexing: Keywords: Cytoplasmic streaming; Egeria densa; actin cytoskeleton; calcium influx; lidocaine; local anesthetic; reactive oxygen species
Substance Nomenclature: 98PI200987 (Lidocaine)
0 (Reactive Oxygen Species)
0 (Anesthetics, Local)
0 (Actins)
SY7Q814VUP (Calcium)
EC 1.6.3.- (NADPH Oxidases)
Entry Date(s): Date Created: 20260916 Date Completed: 20260916 Latest Revision: 20261008
Update Code: 20261008
PubMed Central ID: PMC13596920
DOI: 10.1080/15592324.2026.2726830
PMID: 42746911
Βάση Δεδομένων: MEDLINE
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  Data: Local anesthetic lidocaine decelerates cytoplasmic streaming in Egeria densa via altered actin dynamics and reactive oxygen species generation.
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  Data: <searchLink fieldCode="AU" term="%22Magari+N%22">Magari N</searchLink>; Department of Anesthesiology, Keio University School of Medicine, Shinjuku, Tokyo, Japan.; Faculty of Engineering, Kitami Institute of Technology, Kitami, Hokkaido, Japan.<br /><searchLink fieldCode="AU" term="%22Kagenishi+T%22">Kagenishi T</searchLink>; Faculty of Engineering, Kitami Institute of Technology, Kitami, Hokkaido, Japan.<br /><searchLink fieldCode="AU" term="%22Yokawa+K%22">Yokawa K</searchLink>; Faculty of Engineering, Kitami Institute of Technology, Kitami, Hokkaido, Japan.
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  Data: <searchLink fieldCode="JN" term="%22101291431%22">Plant signaling & behavior</searchLink> [Plant Signal Behav] 2026 Dec 31; Vol. 21 (1), pp. 2726830. <i>Date of Electronic Publication: </i>2026 Sep 16.
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  Data: <i>Publication</i>: 2015- : Philadelphia, PA : Taylor & Francis<br /><i>Original Publication</i>: Georgetown, TX : Landes Bioscience
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  Data: <searchLink fieldCode="MM" term="%22Lidocaine%22">Lidocaine*</searchLink>/<searchLink fieldCode="MM" term="%22Lidocaine+pharmacology%22">pharmacology</searchLink> <br /><searchLink fieldCode="MM" term="%22Reactive+Oxygen+Species%22">Reactive Oxygen Species*</searchLink>/<searchLink fieldCode="MM" term="%22Reactive+Oxygen+Species+metabolism%22">metabolism</searchLink> <br /><searchLink fieldCode="MM" term="%22Anesthetics%2C+Local%22">Anesthetics, Local*</searchLink>/<searchLink fieldCode="MM" term="%22Anesthetics%2C+Local+pharmacology%22">pharmacology</searchLink> <br /><searchLink fieldCode="MM" term="%22Cytoplasmic+Streaming%22">Cytoplasmic Streaming*</searchLink>/<searchLink fieldCode="MM" term="%22Cytoplasmic+Streaming+drug+effects%22">drug effects</searchLink> <br /><searchLink fieldCode="MM" term="%22Actins%22">Actins*</searchLink>/<searchLink fieldCode="MM" term="%22Actins+metabolism%22">metabolism</searchLink> <br /><searchLink fieldCode="MM" term="%22Hydrocharitaceae%22">Hydrocharitaceae*</searchLink>/<searchLink fieldCode="MM" term="%22Hydrocharitaceae+drug+effects%22">drug effects</searchLink> <br /><searchLink fieldCode="MM" term="%22Hydrocharitaceae%22">Hydrocharitaceae*</searchLink>/<searchLink fieldCode="MM" term="%22Hydrocharitaceae+metabolism%22">metabolism</searchLink><br /><searchLink fieldCode="MH" term="%22Calcium%22">Calcium</searchLink>/<searchLink fieldCode="MH" term="%22Calcium+metabolism%22">metabolism</searchLink> ; <searchLink fieldCode="MH" term="%22Cytoplasm%22">Cytoplasm</searchLink>/<searchLink fieldCode="MH" term="%22Cytoplasm+metabolism%22">metabolism</searchLink> ; <searchLink fieldCode="MH" term="%22Cytoplasm%22">Cytoplasm</searchLink>/<searchLink fieldCode="MH" term="%22Cytoplasm+drug+effects%22">drug effects</searchLink> ; <searchLink fieldCode="MH" term="%22NADPH+Oxidases%22">NADPH Oxidases</searchLink>/<searchLink fieldCode="MH" term="%22NADPH+Oxidases+metabolism%22">metabolism</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Local anesthetics (LAs) block voltage-gated Na<superscript>+</superscript> channels in mammals, but they also affect cytoplasmic streaming in plant cells. Although certain LAs inhibit streaming in algae such as Chara, the underlying mechanisms remain unclear. This study aimed to elucidate how LAs reduce cytoplasmic streaming velocity, focusing on the actomyosin system and oxidative stress responses, using lidocaine as a representative LA. Streaming velocity in Egeria densa leaves decreased reversibly after lidocaine treatment and recovered following a washout procedure. Confocal laser scanning microscopy of fluorescently stained actin filaments revealed that lidocaine disrupted F-actin structures. Electron spin resonance analysis showed that lidocaine induced reactive oxygen species (ROS) production in a dose-dependent manner, and spatiotemporal fluorescence analysis with ROS-sensitive probes demonstrated that ROS accumulated near the plasma membrane and increased over time; this process appeared to be mediated by nicotinamide adenine dinucleotide phosphate (NADPH) oxidases and Ca<superscript>2+</superscript> influx. Application of antioxidants attenuated the lidocaine-induced reduction in streaming velocity. In conclusion, lidocaine slows cytoplasmic streaming in E. densa by eliciting a reversible, multifaceted response involving NADPH oxidase-derived ROS, Ca<superscript>2+</superscript> influx, and actomyosin remodeling, offering mechanistic insight into the still poorly understood phenomenon of local anesthetic action in plants.
– Name: Ref
  Label: References
  Group: RefInfo
  Data: Plant Cell Rep. 2024 Nov 24;43(12):293. (PMID: <searchLink fieldCode="PM" term="%2239580775%22">39580775)</searchLink><br />Adv Biol (Weinh). 2025 Dec;9(12):e00486. (PMID: <searchLink fieldCode="PM" term="%2241194452%22">41194452)</searchLink><br />J Cell Biol. 1976 Feb;68(2):395-402. (PMID: <searchLink fieldCode="PM" term="%221245553%22">1245553)</searchLink><br />J Cell Physiol. 1968 Dec;72(3):153-60. (PMID: <searchLink fieldCode="PM" term="%225724566%22">5724566)</searchLink><br />Plant Signal Behav. 2024 Dec 31;19(1):2388443. (PMID: <searchLink fieldCode="PM" term="%2239116108%22">39116108)</searchLink><br />Plant Signal Behav. 2024 Dec 31;19(1):2339574. (PMID: <searchLink fieldCode="PM" term="%2238601988%22">38601988)</searchLink><br />Plant Signal Behav. 2022 Dec 31;17(1):2106410. (PMID: <searchLink fieldCode="PM" term="%2235938584%22">35938584)</searchLink><br />Mitochondrion. 2010 Aug;10(5):487-96. (PMID: <searchLink fieldCode="PM" term="%2220546950%22">20546950)</searchLink><br />Sci Signal. 2009 Aug 18;2(84):ra45. (PMID: <searchLink fieldCode="PM" term="%2219690331%22">19690331)</searchLink><br />J Am Chem Soc. 2013 Apr 10;135(14):5389-98. (PMID: <searchLink fieldCode="PM" term="%2223484901%22">23484901)</searchLink><br />Eur Biophys J. 2005 Nov;34(8):973-86. (PMID: <searchLink fieldCode="PM" term="%2215971063%22">15971063)</searchLink><br />Planta. 1991 Mar;183(4):567-74. (PMID: <searchLink fieldCode="PM" term="%2224193850%22">24193850)</searchLink><br />J Exp Bot. 2003 Jan;54(380):103-13. (PMID: <searchLink fieldCode="PM" term="%2212456760%22">12456760)</searchLink><br />Curr Opin Cell Biol. 2004 Feb;16(1):68-72. (PMID: <searchLink fieldCode="PM" term="%2215037307%22">15037307)</searchLink><br />Ann Bot. 2018 Nov 03;122(5):747-756. (PMID: <searchLink fieldCode="PM" term="%2229236942%22">29236942)</searchLink><br />Anesthesiology. 1990 Apr;72(4):711-34. (PMID: <searchLink fieldCode="PM" term="%222157353%22">2157353)</searchLink><br />Nat Struct Mol Biol. 2025 Feb;32(2):237-242. (PMID: <searchLink fieldCode="PM" term="%2239448849%22">39448849)</searchLink><br />Photosynth Res. 1989 Nov;22(2):137-46. (PMID: <searchLink fieldCode="PM" term="%2224424746%22">24424746)</searchLink><br />Dev Cell. 2013 Nov 11;27(3):345-52. (PMID: <searchLink fieldCode="PM" term="%2224229646%22">24229646)</searchLink><br />J Biol Chem. 2012 Aug 31;287(36):30711-8. (PMID: <searchLink fieldCode="PM" term="%2222740687%22">22740687)</searchLink><br />Lancet. 1968 Dec 21;2(7582):1326-9. (PMID: <searchLink fieldCode="PM" term="%224177393%22">4177393)</searchLink><br />Anesth Prog. 2012 Summer;59(2):90-101; quiz 102-3. (PMID: <searchLink fieldCode="PM" term="%2222822998%22">22822998)</searchLink><br />Plant Physiol. 2000 Jun;123(2):645-54. (PMID: <searchLink fieldCode="PM" term="%2210859194%22">10859194)</searchLink><br />Free Radic Biol Med. 1987;3(4):259-303. (PMID: <searchLink fieldCode="PM" term="%222826304%22">2826304)</searchLink><br />Anesthesiology. 2007 Jul;107(1):136-43. (PMID: <searchLink fieldCode="PM" term="%2217585225%22">17585225)</searchLink><br />Plant Cell. 1998 Feb;10(2):255-66. (PMID: <searchLink fieldCode="PM" term="%229490748%22">9490748)</searchLink><br />J Biol Chem. 2008 Apr 4;283(14):8885-92. (PMID: <searchLink fieldCode="PM" term="%2218218618%22">18218618)</searchLink><br />Front Plant Sci. 2020 Jun 30;11:968. (PMID: <searchLink fieldCode="PM" term="%2232695131%22">32695131)</searchLink><br />Korean J Physiol Pharmacol. 2012 Dec;16(6):413-22. (PMID: <searchLink fieldCode="PM" term="%2223269904%22">23269904)</searchLink><br />Proc Natl Acad Sci U S A. 1974 Nov;71(11):4457-61. (PMID: <searchLink fieldCode="PM" term="%224530994%22">4530994)</searchLink><br />Free Radic Biol Med. 2018 Jul;122:21-27. (PMID: <searchLink fieldCode="PM" term="%2229203327%22">29203327)</searchLink><br />Sci Adv. 2025 Dec 5;11(49):eadw6915. (PMID: <searchLink fieldCode="PM" term="%2241337577%22">41337577)</searchLink><br />Nature. 1982 Apr 15;296(5858):647-50. (PMID: <searchLink fieldCode="PM" term="%227070508%22">7070508)</searchLink><br />Nat Plants. 2015 Mar 23;1(4):15031. (PMID: <searchLink fieldCode="PM" term="%2227247032%22">27247032)</searchLink><br />Curr Opin Plant Biol. 2015 Oct;27:104-10. (PMID: <searchLink fieldCode="PM" term="%2226202096%22">26202096)</searchLink><br />Plant J. 2005 Oct;44(2):342-7. (PMID: <searchLink fieldCode="PM" term="%2216212611%22">16212611)</searchLink>
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  Data: <i>Keywords: </i>Cytoplasmic streaming; Egeria densa; actin cytoskeleton; calcium influx; lidocaine; local anesthetic; reactive oxygen species
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        Text: English
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        StartPage: 2726830
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      – SubjectFull: Calcium metabolism
        Type: general
      – SubjectFull: Cytoplasm metabolism
        Type: general
      – SubjectFull: Cytoplasm drug effects
        Type: general
      – SubjectFull: NADPH Oxidases metabolism
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      – SubjectFull: Anesthetics, Local pharmacology
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      – SubjectFull: Cytoplasmic Streaming drug effects
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      – SubjectFull: Hydrocharitaceae drug effects
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      – TitleFull: Local anesthetic lidocaine decelerates cytoplasmic streaming in Egeria densa via altered actin dynamics and reactive oxygen species generation.
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