Local anesthetic lidocaine decelerates cytoplasmic streaming in Egeria densa via altered actin dynamics and reactive oxygen species generation.

Λεπτομέρειες βιβλιογραφικής εγγραφής
Τίτλος:	Local anesthetic lidocaine decelerates cytoplasmic streaming in Egeria densa via altered actin dynamics and reactive oxygen species generation.
Συγγραφείς:	Magari N; Department of Anesthesiology, Keio University School of Medicine, Shinjuku, Tokyo, Japan.; Faculty of Engineering, Kitami Institute of Technology, Kitami, Hokkaido, Japan., Kagenishi T; Faculty of Engineering, Kitami Institute of Technology, Kitami, Hokkaido, Japan., Yokawa K; Faculty of Engineering, Kitami Institute of Technology, Kitami, Hokkaido, Japan.
Πηγή:	Plant signaling & behavior [Plant Signal Behav] 2026 Dec 31; Vol. 21 (1), pp. 2726830. Date of Electronic Publication: 2026 Sep 16.
Τύπος έκδοσης:	Journal Article
Γλώσσα:	English
Στοιχεία περιοδικού:	Publisher: Taylor & Francis Country of Publication: United States NLM ID: 101291431 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1559-2324 (Electronic) Linking ISSN: 15592316 NLM ISO Abbreviation: Plant Signal Behav Subsets: MEDLINE
Imprint Name(s):	Publication: 2015- : Philadelphia, PA : Taylor & Francis Original Publication: Georgetown, TX : Landes Bioscience
Ιατρικοί όροι (MeSH):	Lidocaine/pharmacology , Reactive Oxygen Species/metabolism , Anesthetics, Local/pharmacology , Cytoplasmic Streaming/drug effects , Actins/metabolism , Hydrocharitaceae/drug effects , Hydrocharitaceae*/metabolism, Calcium/metabolism ; Cytoplasm/metabolism ; Cytoplasm/drug effects ; NADPH Oxidases/metabolism
Περίληψη:	Local anesthetics (LAs) block voltage-gated Na⁺ channels in mammals, but they also affect cytoplasmic streaming in plant cells. Although certain LAs inhibit streaming in algae such as Chara, the underlying mechanisms remain unclear. This study aimed to elucidate how LAs reduce cytoplasmic streaming velocity, focusing on the actomyosin system and oxidative stress responses, using lidocaine as a representative LA. Streaming velocity in Egeria densa leaves decreased reversibly after lidocaine treatment and recovered following a washout procedure. Confocal laser scanning microscopy of fluorescently stained actin filaments revealed that lidocaine disrupted F-actin structures. Electron spin resonance analysis showed that lidocaine induced reactive oxygen species (ROS) production in a dose-dependent manner, and spatiotemporal fluorescence analysis with ROS-sensitive probes demonstrated that ROS accumulated near the plasma membrane and increased over time; this process appeared to be mediated by nicotinamide adenine dinucleotide phosphate (NADPH) oxidases and Ca²⁺ influx. Application of antioxidants attenuated the lidocaine-induced reduction in streaming velocity. In conclusion, lidocaine slows cytoplasmic streaming in E. densa by eliciting a reversible, multifaceted response involving NADPH oxidase-derived ROS, Ca²⁺ influx, and actomyosin remodeling, offering mechanistic insight into the still poorly understood phenomenon of local anesthetic action in plants.
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Contributed Indexing:	Keywords: Cytoplasmic streaming; Egeria densa; actin cytoskeleton; calcium influx; lidocaine; local anesthetic; reactive oxygen species
Substance Nomenclature:	98PI200987 (Lidocaine) 0 (Reactive Oxygen Species) 0 (Anesthetics, Local) 0 (Actins) SY7Q814VUP (Calcium) EC 1.6.3.- (NADPH Oxidases)
Entry Date(s):	Date Created: 20260916 Date Completed: 20260916 Latest Revision: 20261008
Update Code:	20261008
PubMed Central ID:	PMC13596920
DOI:	10.1080/15592324.2026.2726830
PMID:	42746911
Βάση Δεδομένων:	MEDLINE

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ISSN:	1559-2324
DOI:	10.1080/15592324.2026.2726830