MBD3 Knockdown Promotes the Differentiation of Bovine iPSCs into Primordial Germ Cell-like Cells Associated with Altered Bivalent Modification of TGF-β-Related Genes.

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Τίτλος:	MBD3 Knockdown Promotes the Differentiation of Bovine iPSCs into Primordial Germ Cell-like Cells Associated with Altered Bivalent Modification of TGF-β-Related Genes.
Συγγραφείς:	Yuan W; State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, College of Life Science, Inner Mongolia University, Hohhot 010021, China.; College of Life Science and Technology, Inner Mongolia Normal University, Hohhot 010022, China., Wang J; State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, College of Life Science, Inner Mongolia University, Hohhot 010021, China., Li W; State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, College of Life Science, Inner Mongolia University, Hohhot 010021, China., Luo Y; State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, College of Life Science, Inner Mongolia University, Hohhot 010021, China., Liu Y; State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, College of Life Science, Inner Mongolia University, Hohhot 010021, China., Long S; State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, College of Life Science, Inner Mongolia University, Hohhot 010021, China., Wang Y; State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, College of Life Science, Inner Mongolia University, Hohhot 010021, China., Liu F; State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, College of Life Science, Inner Mongolia University, Hohhot 010021, China., Geng X; State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, College of Life Science, Inner Mongolia University, Hohhot 010021, China., Yue Y; State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, College of Life Science, Inner Mongolia University, Hohhot 010021, China., Li X; State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, College of Life Science, Inner Mongolia University, Hohhot 010021, China.
Πηγή:	Cells [Cells] 2026 Aug 30; Vol. 15 (17). Date of Electronic Publication: 2026 Aug 30.
Τύπος έκδοσης:	Journal Article
Γλώσσα:	English
Στοιχεία περιοδικού:	Publisher: MDPI Country of Publication: Switzerland NLM ID: 101600052 Publication Model: Electronic Cited Medium: Internet ISSN: 2073-4409 (Electronic) Linking ISSN: 20734409 NLM ISO Abbreviation: Cells Subsets: MEDLINE
Imprint Name(s):	Original Publication: Basel, Switzerland : MDPI
Ιατρικοί όροι (MeSH):	Cell Differentiation/genetics , Transforming Growth Factor beta/metabolism , Transforming Growth Factor beta/genetics , Germ Cells/cytology , Germ Cells/metabolism , DNA-Binding Proteins/metabolism , DNA-Binding Proteins/genetics , Induced Pluripotent Stem Cells/metabolism , Induced Pluripotent Stem Cells/cytology , Transcription Factors/metabolism , Transcription Factors/genetics , Gene Knockdown Techniques, Animals ; Cattle ; Signal Transduction
Περίληψη:	Methyl-CpG binding domain protein 3 (MBD3) is a member of the nucleosome remodeling and deacetylase (NuRD) corepressor protein complex, which plays a pivotal role in embryonic development, pluripotent stem cell (PSC) differentiation, and induced pluripotent stem cell (iPSC) reprogramming. However, whether MBD3 is also involved in iPSC differentiation into primordial germ cell-like cells (PGCLCs) is unclear. In this study, bovine iPSCs generated by MBD3 knockdown (MBD3 KD) during reprogramming were subjected to PGCLC differentiation using a monolayer cell culture method. Our results revealed that MBD3 KD enhanced the ability of bovine iPSCs to differentiate into PGCLCs. MBD3-KD bovine iPSC-derived PGCLCs exhibited the characteristics of in vivo primordial germ cells (PGCs) in a migratory state. MBD3 KD increased NODAL signal transduction during the induction of PGCLCs and was associated with increased expression of PRDM1 and SOX15. Moreover, MBD3 KD increased the bivalent modification of transforming growth factor beta (TGF-β)-related genes concomitant with the upregulation of DPPA4 and DNMT3L expression in bovine iPSCs, and this epigenetic change was associated with enhanced NODAL signaling. Our results indicate that MBD3 KD during reprogramming favors the differentiation of bovine iPSCs into PGCLCs, further highlighting the impact of MBD3 on histone modifications during germ cell development.
References:	iScience. 2020 Dec 29;24(1):102003. (PMID: 33490911) Stem Cells. 2011 Jul;29(7):1041-51. (PMID: 21608077) Cold Spring Harb Perspect Biol. 2017 Jan 3;9(1):. (PMID: 27836834) Cell Rep. 2022 Nov 1;41(5):111587. (PMID: 36323261) Nature. 2017 Jun 15;546(7658):416-420. (PMID: 28607482) Life Sci Alliance. 2021 Feb 19;4(5):. (PMID: 33608411) Nat Methods. 2015 Apr;12(4):357-60. (PMID: 25751142) Wellcome Open Res. 2019 Aug 16;4:122. (PMID: 31583280) Bioinformatics. 2009 Jul 15;25(14):1754-60. (PMID: 19451168) Sci Rep. 2017 Mar 03;7:43851. (PMID: 28256590) Cell Discov. 2023 Feb 3;9(1):14. (PMID: 36737434) Cell. 2013 Sep 26;155(1):121-34. (PMID: 24074865) Cell. 2015 Jun 4;161(6):1437-52. (PMID: 26046443) Nature. 2013 Oct 3;502(7469):65-70. (PMID: 24048479) Cell Rep. 2013 Jun 27;3(6):1777-84. (PMID: 23727241) Stem Cell Reports. 2021 Dec 14;16(12):2844-2851. (PMID: 34767751) J Biol Chem. 2015 Aug 14;290(33):20105-16. (PMID: 26126825) Elife. 2022 Apr 08;11:. (PMID: 35394424) Cell Stem Cell. 2017 Oct 5;21(4):517-532.e5. (PMID: 28985527) OMICS. 2012 May;16(5):284-7. (PMID: 22455463) Nature. 2016 Sep 22;537(7621):558-562. (PMID: 27626379) J Virol. 2014 Sep;88(18):10680-95. (PMID: 24991018) Reproduction. 2006 Aug;132(2):233-46. (PMID: 16885532) Nat Struct Mol Biol. 2020 Aug;27(8):696-705. (PMID: 32572255) Development. 2024 Apr 1;151(7):. (PMID: 38607588) Reproduction. 2015 Oct;150(4):245-56. (PMID: 26159833) Int J Mol Sci. 2023 Mar 21;24(6):. (PMID: 36982982) J Cell Mol Med. 2021 Apr;25(8):4014-4027. (PMID: 33724652) Cancer Lett. 2024 Jun 1;591:216902. (PMID: 38641310) Dev Biol. 2005 Aug 1;284(1):194-203. (PMID: 15993878) Nature. 2018 Oct;562(7728):595-599. (PMID: 30283136) Proc Natl Acad Sci U S A. 2021 Jun 8;118(23):. (PMID: 34088849) Reproduction. 2021 Mar;161(3):239-253. (PMID: 33275120) Bioinformatics. 2010 Jan 1;26(1):139-40. (PMID: 19910308) Genome Biol. 2008;9(9):R137. (PMID: 18798982) Bioinformatics. 2015 Jul 15;31(14):2382-3. (PMID: 25765347) FEBS J. 2021 Jul;288(14):4394-4411. (PMID: 33524211) Nature. 2011 Jan 13;469(7329):231-5. (PMID: 21196933) Nucleic Acids Res. 2002 Sep 1;30(17):3831-8. (PMID: 12202768) J Reprod Dev. 2024 Apr 4;70(2):82-95. (PMID: 38355134) Nat Struct Mol Biol. 2020 Aug;27(8):685-686. (PMID: 32572253) Semin Cell Dev Biol. 2015 Sep;45:94-103. (PMID: 26500180) Cells. 2024 Feb 23;13(5):. (PMID: 38474345) Cell Stem Cell. 2015 Aug 6;17(2):178-94. (PMID: 26189426) Proc Natl Acad Sci U S A. 2013 Oct 1;110(40):16061-6. (PMID: 24043772)
Grant Information:	32160172 National Natural Science Foundation of China; 2023KYPT0010 Key Science-Technology Project of Inner Mongolia; 2026CXPT02003 Key Science-Technology Project of Inner Mongolia; 2023ZD0407504 STI 2030-Major Projects of China; NMGIRT2204 Development Plan for Young Scientific and Technological Talents in Colleges and Universities of Inner Mongolia Autonomous Region of China
Contributed Indexing:	Keywords: MBD3; PGCLCs; bivalent
Substance Nomenclature:	0 (Transforming Growth Factor beta) 0 (DNA-Binding Proteins) 0 (Transcription Factors)
Entry Date(s):	Date Created: 20260915 Date Completed: 20260915 Latest Revision: 20260916
Update Code:	20260916
PubMed Central ID:	PMC13564770
DOI:	10.3390/cells15171577
PMID:	42738871
Βάση Δεδομένων:	MEDLINE

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ISSN:	2073-4409
DOI:	10.3390/cells15171577