Bioorthogonal Click Chemistry Enables Stable Cell Attachment to Silk Fibroin Scaffolds for Tissue Engineering Applications: Proof of Concept in Neural Regeneration.

Λεπτομέρειες βιβλιογραφικής εγγραφής
Τίτλος:	Bioorthogonal Click Chemistry Enables Stable Cell Attachment to Silk Fibroin Scaffolds for Tissue Engineering Applications: Proof of Concept in Neural Regeneration.
Συγγραφείς:	Quintana-Prego A; Center for Biomedical Technology, Universidad Politécnica de Madrid, Pozuelo de Alarcón, 28223Madrid, Spain.; Silk Biomed SL, 28260Madrid, Spain., Guedan-Duran A; Bioactive Surfaces SL, 28260Madrid, Spain., Guinea GV; Center for Biomedical Technology, Universidad Politécnica de Madrid, Pozuelo de Alarcón, 28223Madrid, Spain.; Silk Biomed SL, 28260Madrid, Spain.; Bioactive Surfaces SL, 28260Madrid, Spain.; Biomaterials and Regenerative Medicine Group, Instituto de Investigación Sanitaria del Hospital Clínico San Carlos (IdISSC), 28040Madrid, Spain.; Department of Material Science, ETSI Caminos, Canales y Puertos, Universidad Politécnica de Madrid, 28040Madrid, Spain.; Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Instituto de Salud Carlos III, 28029Madrid, Spain., Gomez-Rivas J; Urology Service, Instituto de Investigación Sanitaria del Hospital Clínico San Carlos (IdISSC), 28040Madrid, Spain., Panetsos F; Silk Biomed SL, 28260Madrid, Spain.; Neurocomputing and Neurorobotics Research Group, Faculty of Biology and Faculty of Optics, Complutense University of Madrid, 28040Madrid, Spain.; Bioactive Surfaces SL, 28260Madrid, Spain.; Omnia Mater SL, 28009Madrid, Spain.; Human Retina SL, 28009Madrid, Spain.
Πηγή:	Biomacromolecules [Biomacromolecules] 2026 Sep 14; Vol. 27 (9), pp. 6096-6110.
Τύπος έκδοσης:	Journal Article
Γλώσσα:	English
Στοιχεία περιοδικού:	Publisher: American Chemical Society Country of Publication: United States NLM ID: 100892849 Publication Model: Print Cited Medium: Internet ISSN: 1526-4602 (Electronic) Linking ISSN: 15257797 NLM ISO Abbreviation: Biomacromolecules Subsets: MEDLINE
Imprint Name(s):	Original Publication: Washington, DC : American Chemical Society, c2000-
Ιατρικοί όροι (MeSH):	Click Chemistry/methods , Tissue Engineering/methods , Tissue Scaffolds/chemistry , Fibroins/chemistry , Nerve Regeneration*, Schwann Cells/cytology ; Schwann Cells/metabolism ; Azides/chemistry ; Animals ; Cell Adhesion ; Rats
Περίληψη:	Tissue engineering seeks effective strategies to integrate cells into scaffolds while preserving spatial organization and stability. Conventional approaches based on extracellular matrix molecules or adhesion peptides often provide weak and nonspecific attachments, limiting robust assembly of complex cellular architectures. Here, we investigate biorthogonal click chemistry to address these limitations. By enabling covalent cell-biomaterial bonding through strain-promoted azide-alkyne cycloaddition (SPAAC), we achieved stable cell-scaffold integration. As a proof of concept, we engineered artificial Bands of Büngner-like scaffolds by functionalizing silk microfibers with cyclooctyne groups and metabolically engineering Schwann cells to present azide functionalities. Over 15 days in vitro, these biohybrid scaffolds supported directed neurite outgrowth, preserved metabolic activity, and increased nerve growth factor levels at early time points. Our results support click chemistry as a robust strategy for assembling stable cell-laden scaffolds while preserving biological function, potentially enabling the tailored design of complex cellular architectures and microenvironments for tissue engineering applications. (© 2026 The Authors. Published by American Chemical Society.)
References:	Angew Chem Int Ed Engl. 2001 Jun 1;40(11):2004-2021. (PMID: 11433435) ACS Nano. 2018 Dec 26;12(12):12193-12200. (PMID: 30511851) Mater Today Bio. 2022 Dec 28;18:100530. (PMID: 36601535) Angew Chem Int Ed Engl. 2010 Apr 26;49(19):3375-8. (PMID: 20391522) Nat Neurosci. 2010 Jul;13(7):897-905. (PMID: 20543841) Chem Soc Rev. 2010 Apr;39(4):1272-9. (PMID: 20349533) Acta Biomater. 2019 Sep 15;96:175-187. (PMID: 31260823) J Bone Joint Surg Am. 2007 Apr;89(4):786-91. (PMID: 17403801) Materials (Basel). 2015 Aug 28;8(9):5671-5701. (PMID: 28793529) Science. 1993 May 14;260(5110):920-6. (PMID: 8493529) Bioact Mater. 2021 Sep 23;10:15-31. (PMID: 34901526) Adv Mater. 2022 Feb;34(5):e2107392. (PMID: 34775659) Cells Tissues Organs. 2014;200(1):6-12. (PMID: 25765065) Annu Rev Chem Biomol Eng. 2011;2:403-30. (PMID: 22432625) J Physiol. 2016 Jul 1;594(13):3521-31. (PMID: 26864683) Angew Chem Int Ed Engl. 2012 Nov 19;51(47):11836-40. (PMID: 23081905) Ann Biomed Eng. 2020 Jul;48(7):2132-2153. (PMID: 31741227) Biomaterials. 2003 Nov;24(24):4337-51. (PMID: 12922147) Acta Biomater. 2022 Oct 1;151:106-117. (PMID: 35970482) Adv Healthc Mater. 2018 Oct;7(19):e1800560. (PMID: 30106514) Science. 2008 May 2;320(5876):664-7. (PMID: 18451302) Urology. 2008 Nov;72(5):1036-9. (PMID: 18799206) Proc Natl Acad Sci U S A. 2016 May 10;113(19):5173-8. (PMID: 27125855) Biomacromolecules. 2017 Apr 10;18(4):1127-1133. (PMID: 28226209) Proc Natl Acad Sci U S A. 2010 Feb 2;107(5):1821-6. (PMID: 20080615) Mol Neurobiol. 1997 Feb-Apr;14(1-2):67-116. (PMID: 9170101) Nat Chem Biol. 2017 Apr;13(4):415-424. (PMID: 28192414) Int J Mol Sci. 2021 Dec 02;22(23):. (PMID: 34884856) Adv Wound Care (New Rochelle). 2024 Dec;13(12):625-638. (PMID: 38775424) Mol Neurobiol. 2003 Jun;27(3):277-324. (PMID: 12845152) Angew Chem Int Ed Engl. 2016 Jun 27;55(27):7660-4. (PMID: 27080423) J Shoulder Elbow Surg. 2004 Sep-Oct;13(5):538-41. (PMID: 15383811) J Mater Chem. 2009 Jun 23;19(36):6443-6450. (PMID: 20161439) Biomaterials. 2020 Feb;230:119615. (PMID: 31776020) ACS Nano. 2009 May 26;3(5):1151-9. (PMID: 19397333) J Surg Res. 2012 Aug;176(2):490-502. (PMID: 22341350) J R Soc Interface. 2007 Dec 22;4(17):999-1030. (PMID: 17412675) Acta Orthop Belg. 2007 Aug;73(4):432-6. (PMID: 17939470) J Am Chem Soc. 2014 Dec 17;136(50):17468-76. (PMID: 25313651) Oncotarget. 2016 Sep 6;7(36):58671-58683. (PMID: 27486772) Biomaterials. 2014 Jan;35(1):185-95. (PMID: 24120048) Nat Chem. 2014 May;6(5):393-403. (PMID: 24755590) Sci Adv. 2023 Feb 10;9(6):eade8829. (PMID: 36753555)
Grant Information:	B2017/BMD-3760 Comunidad de Madrid; P022/BMD-7236 Comunidad de Madrid; PIPF-2022_SAL-GL-25129 Comunidad de Madrid; PI22/00916 Ministerio de Ciencia, Innovaci?n y Universidades; 101099719 HORIZON EUROPE European Innovation Council; 101130454 HORIZON EUROPE European Innovation Council
Substance Nomenclature:	9007-76-5 (Fibroins) 0 (Azides)
Entry Date(s):	Date Created: 20260914 Date Completed: 20260914 Latest Revision: 20260918
Update Code:	20260918
PubMed Central ID:	PMC13580028
DOI:	10.1021/acs.biomac.6c01009
PMID:	42734291
Βάση Δεδομένων:	MEDLINE

Περιγραφή
ISSN:	1526-4602
DOI:	10.1021/acs.biomac.6c01009