Academic Journal
Cell-cycle-resolved τSTED nanoscopy reveals nanoscale reorganization of TGN46-positive structures.
| Τίτλος: | Cell-cycle-resolved τSTED nanoscopy reveals nanoscale reorganization of TGN46-positive structures. |
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| Συγγραφείς: | Katoh K; Exploratory Research Center on Life and Living Systems (ExCELLS), National Institutes of Natural Sciences.; Artificial Intelligence Research Center, National Institute of Advanced Industrial Science and Technology (AIST)., Tateo S; Exploratory Research Center on Life and Living Systems (ExCELLS), National Institutes of Natural Sciences.; Institute for Molecular Science, National Institutes of Natural Sciences.; Faculty and Graduate School of Pharmaceutical Sciences, Nagoya City University., Mitsuyama T; Exploratory Research Center on Life and Living Systems (ExCELLS), National Institutes of Natural Sciences.; Artificial Intelligence Research Center, National Institute of Advanced Industrial Science and Technology (AIST)., Kato K; Exploratory Research Center on Life and Living Systems (ExCELLS), National Institutes of Natural Sciences.; Institute for Molecular Science, National Institutes of Natural Sciences.; Faculty and Graduate School of Pharmaceutical Sciences, Nagoya City University.; Graduate Institute for Advanced Studies, SOKENDAI. |
| Πηγή: | Cell structure and function [Cell Struct Funct] 2026 Oct 08; Vol. 51 (2), pp. 263-275. Date of Electronic Publication: 2026 Sep 12. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Japan Society for Cell Biology Country of Publication: Japan NLM ID: 7608465 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1347-3700 (Electronic) Linking ISSN: 03867196 NLM ISO Abbreviation: Cell Struct Funct Subsets: MEDLINE |
| Imprint Name(s): | Publication: Tokyo : Japan Society for Cell Biology Original Publication: Okayama, Japan, Japan Society for Cell Biology. |
| Ιατρικοί όροι (MeSH): | Golgi Apparatus*/metabolism , Cell Cycle* , Nanotechnology*, trans-Golgi Network/metabolism ; Humans ; HeLa Cells ; Microscopy, Fluorescence |
| Περίληψη: | The Golgi apparatus is a highly organized membrane system whose structure is remodeled during cell-cycle progression, but nanoscale changes in individual Golgi subdomains during interphase remain incompletely understood. Here, we used Fucci-compatible fluorescence lifetime-based STED (τSTED) nanoscopy to examine cell-cycle-associated reorganization of cis-Golgi and trans-Golgi network (TGN)-associated structures in fixed HeLa cells. By combining Fucci-SA reporters with red/far-red Golgi labeling, large-field sequential imaging, Abberior STAR RED nanobody labeling, and reproducible field-wise lifetime-trajectory-based τSTED reconstruction, we correlated Fucci-defined cell-cycle state with nanoscale Golgi morphology in individual cells. Automated morphometry showed that GM130-positive cis-Golgi and TGN46-positive trans/TGN-associated distribution domains were relatively compact in G1 cells, whereas TGN46-positive structures exhibited an expanded spatial distribution in G2-enriched cells. Optimized τSTED imaging resolved this expansion as dispersed submicron TGN46-positive structures rather than uniform enlargement of the Golgi apparatus. These findings reveal cell-cycle-associated spatial reorganization of TGN46-positive trans/TGN-associated structures and demonstrate the utility of cell-cycle-resolved super-resolution imaging for analyzing organelle architecture.Key words: Golgi apparatus, cell cycling, super-resolution microscopy, τSTED, Fucci. |
| Contributed Indexing: | Keywords: Fucci; Golgi apparatus; cell cycling; super-resolution microscopy; τSTED |
| Entry Date(s): | Date Created: 20260913 Date Completed: 20261007 Latest Revision: 20261007 |
| Update Code: | 20261008 |
| DOI: | 10.1247/csf.26065 |
| PMID: | 42732940 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1347-3700 |
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| DOI: | 10.1247/csf.26065 |