Academic Journal
View in EDS
Linked Full Text

Ultrasound-assisted extraction of polyphenols from Moutan Cortex using natural deep eutectic solvent: Optimization, molecular mechanisms, and lipid-lowering activity.

Bibliographic Details
Title: Ultrasound-assisted extraction of polyphenols from Moutan Cortex using natural deep eutectic solvent: Optimization, molecular mechanisms, and lipid-lowering activity.
Authors: Yin S; Jiangsu Agri-animal Husbandry Vocational College, Taizhou 225300, PR China., Peng W; School of Veterinary Medicine, Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225009, PR China; Joint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou 225009, PR China., Wang Y; Jiangsu Agri-animal Husbandry Vocational College, Taizhou 225300, PR China., Lu H; Jiangsu Agri-animal Husbandry Vocational College, Taizhou 225300, PR China., Liu P; Jiangsu Agri-animal Husbandry Vocational College, Taizhou 225300, PR China., Zhu X; Jiangsu Agri-animal Husbandry Vocational College, Taizhou 225300, PR China., Yang H; Jiangsu Agri-animal Husbandry Vocational College, Taizhou 225300, PR China., Li J; School of Veterinary Medicine, Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225009, PR China; Joint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou 225009, PR China. Electronic address: jgli@yzu.edu.cn., Li L; Jiangsu Agri-animal Husbandry Vocational College, Taizhou 225300, PR China. Electronic address: liliuhui01@163.com.
Source: Ultrasonics sonochemistry [Ultrason Sonochem] 2026 Oct; Vol. 133, pp. 108052. Date of Electronic Publication: 2026 Sep 10.
Publication Type: Journal Article
Language: English
Journal Info: Publisher: Elsevier Science Country of Publication: Netherlands NLM ID: 9433356 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2828 (Electronic) Linking ISSN: 13504177 NLM ISO Abbreviation: Ultrason Sonochem Subsets: MEDLINE
Imprint Name(s): Publication: Amsterdam : Elsevier Science
Original Publication: Oxford : Butterworth Heinemann, c1994-
MeSH Terms: Polyphenols*/isolation & purification , Polyphenols*/pharmacology , Polyphenols*/chemistry , Paeonia*/chemistry , Chemical Fractionation*/methods , Deep Eutectic Solvents*/chemistry , Drugs, Chinese Herbal*/chemistry , Ultrasonic Waves*, Solvents/chemistry ; Molecular Dynamics Simulation ; Animals
Abstract: Moutan Cortex is a valuable medicinal resource rich in bioactive polyphenols, yet its sustainable valorization is often hindered by the inefficiencies and environmental concerns associated with conventional extraction methods. In this study, a green and efficient ultrasound-assisted extraction (UAE) strategy was developed using a betaine-levulinic acid (Bet-LevA) natural deep eutectic solvent (NADES). An intelligent optimization approach was applied to determine the optimal extraction conditions, achieving a maximum total polyphenol content of 27.03 ± 0.41 mg GAE/g DW, which was 1.93-fold higher than that obtained using 70% ethanol extraction. The molecular mechanisms underlying the superior extraction performance of Bet-LevA were further elucidated using FT-IR, conductor-like screening model for realistic solvents (COSMO-RS), and molecular dynamics (MD) simulations, revealing that hydrogen-bond networks and stronger solvent-polyphenol interactions promoted solubilization and stabilization of phenolic compounds. Furthermore, the extracted polyphenol fraction effectively alleviated fatty acid-induced lipid accumulation in AML-12 hepatocytes. Overall, this study provides a sustainable extraction framework integrating green solvent design, intelligent optimization, molecular mechanism analysis, and functional evaluation for the high-value utilization of Moutan Cortex in functional foods and nutraceutical applications.
(Copyright © 2026 The Authors. Published by Elsevier B.V. All rights reserved.)
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
Contributed Indexing: Keywords: COSMO-RS; Lipid-lowering activity; Molecular dynamics simulation; MoutanCortex; Natural deep eutectic solvents; Polyphenols; Ultrasound-assisted extraction
Substance Nomenclature: 0 (Polyphenols)
0 (moutan cortex)
0 (Deep Eutectic Solvents)
0 (Drugs, Chinese Herbal)
0 (Solvents)
Entry Date(s): Date Created: 20260912 Date Completed: 20261005 Latest Revision: 20261005
Update Code: 20261006
PubMed Central ID: PMC13587915
DOI: 10.1016/j.ultsonch.2026.108052
PMID: 42731572
Database: MEDLINE
FullText Links:
  – Type: other
    Url: https://resolver.ebsco.com:443/public/rma-ftfapi/ejs/direct?AccessToken=43BDB79AA866D8AEEA58&Show=Object
Text:
  Availability: 0
CustomLinks:
  – Url: https://www.doi.org/10.1016/j.ultsonch.2026.108052?
    Name: ScienceDirect (all content) (s7799221)
    Category: fullText
    Text: View record from ScienceDirect
    MouseOverText: View record from ScienceDirect
Header DbId: cmedm
DbLabel: MEDLINE
An: 42731572
AccessLevel: 3
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: Ultrasound-assisted extraction of polyphenols from Moutan Cortex using natural deep eutectic solvent: Optimization, molecular mechanisms, and lipid-lowering activity.
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AU" term="%22Yin+S%22">Yin S</searchLink>; Jiangsu Agri-animal Husbandry Vocational College, Taizhou 225300, PR China.<br /><searchLink fieldCode="AU" term="%22Peng+W%22">Peng W</searchLink>; School of Veterinary Medicine, Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225009, PR China; Joint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou 225009, PR China.<br /><searchLink fieldCode="AU" term="%22Wang+Y%22">Wang Y</searchLink>; Jiangsu Agri-animal Husbandry Vocational College, Taizhou 225300, PR China.<br /><searchLink fieldCode="AU" term="%22Lu+H%22">Lu H</searchLink>; Jiangsu Agri-animal Husbandry Vocational College, Taizhou 225300, PR China.<br /><searchLink fieldCode="AU" term="%22Liu+P%22">Liu P</searchLink>; Jiangsu Agri-animal Husbandry Vocational College, Taizhou 225300, PR China.<br /><searchLink fieldCode="AU" term="%22Zhu+X%22">Zhu X</searchLink>; Jiangsu Agri-animal Husbandry Vocational College, Taizhou 225300, PR China.<br /><searchLink fieldCode="AU" term="%22Yang+H%22">Yang H</searchLink>; Jiangsu Agri-animal Husbandry Vocational College, Taizhou 225300, PR China.<br /><searchLink fieldCode="AU" term="%22Li+J%22">Li J</searchLink>; School of Veterinary Medicine, Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225009, PR China; Joint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou 225009, PR China. Electronic address: jgli@yzu.edu.cn.<br /><searchLink fieldCode="AU" term="%22Li+L%22">Li L</searchLink>; Jiangsu Agri-animal Husbandry Vocational College, Taizhou 225300, PR China. Electronic address: liliuhui01@163.com.
– Name: TitleSource
  Label: Source
  Group: Src
  Data: <searchLink fieldCode="JN" term="%229433356%22">Ultrasonics sonochemistry</searchLink> [Ultrason Sonochem] 2026 Oct; Vol. 133, pp. 108052. <i>Date of Electronic Publication: </i>2026 Sep 10.
– Name: TypePub
  Label: Publication Type
  Group: TypPub
  Data: Journal Article
– Name: Language
  Label: Language
  Group: Lang
  Data: English
– Name: TitleSource
  Label: Journal Info
  Group: Src
  Data: <i>Publisher: </i><searchLink fieldCode="PB" term="%22Elsevier+Science%22">Elsevier Science </searchLink><i>Country of Publication: </i>Netherlands <i>NLM ID: </i>9433356 <i>Publication Model: </i>Print-Electronic <i>Cited Medium: </i>Internet <i>ISSN: </i>1873-2828 (Electronic) <i>Linking ISSN: </i><searchLink fieldCode="IS" term="%2213504177%22">13504177 </searchLink><i>NLM ISO Abbreviation: </i>Ultrason Sonochem <i>Subsets: </i>MEDLINE
– Name: PublisherInfo
  Label: Imprint Name(s)
  Group: PubInfo
  Data: <i>Publication</i>: Amsterdam : Elsevier Science<br /><i>Original Publication</i>: Oxford : Butterworth Heinemann, c1994-
– Name: SubjectMESH
  Label: MeSH Terms
  Group: Su
  Data: <searchLink fieldCode="MM" term="%22Polyphenols%22">Polyphenols*</searchLink>/<searchLink fieldCode="MM" term="%22Polyphenols+isolation+%26+purification%22">isolation & purification</searchLink> <br /><searchLink fieldCode="MM" term="%22Polyphenols%22">Polyphenols*</searchLink>/<searchLink fieldCode="MM" term="%22Polyphenols+pharmacology%22">pharmacology</searchLink> <br /><searchLink fieldCode="MM" term="%22Polyphenols%22">Polyphenols*</searchLink>/<searchLink fieldCode="MM" term="%22Polyphenols+chemistry%22">chemistry</searchLink> <br /><searchLink fieldCode="MM" term="%22Paeonia%22">Paeonia*</searchLink>/<searchLink fieldCode="MM" term="%22Paeonia+chemistry%22">chemistry</searchLink> <br /><searchLink fieldCode="MM" term="%22Chemical+Fractionation%22">Chemical Fractionation*</searchLink>/<searchLink fieldCode="MM" term="%22Chemical+Fractionation+methods%22">methods</searchLink> <br /><searchLink fieldCode="MM" term="%22Deep+Eutectic+Solvents%22">Deep Eutectic Solvents*</searchLink>/<searchLink fieldCode="MM" term="%22Deep+Eutectic+Solvents+chemistry%22">chemistry</searchLink> <br /><searchLink fieldCode="MM" term="%22Drugs%2C+Chinese+Herbal%22">Drugs, Chinese Herbal*</searchLink>/<searchLink fieldCode="MM" term="%22Drugs%2C+Chinese+Herbal+chemistry%22">chemistry</searchLink> <br /><searchLink fieldCode="MM" term="%22Ultrasonic+Waves%22">Ultrasonic Waves*</searchLink><br /><searchLink fieldCode="MH" term="%22Solvents%22">Solvents</searchLink>/<searchLink fieldCode="MH" term="%22Solvents+chemistry%22">chemistry</searchLink> ; <searchLink fieldCode="MH" term="%22Molecular+Dynamics+Simulation%22">Molecular Dynamics Simulation</searchLink> ; <searchLink fieldCode="MH" term="%22Animals%22">Animals</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Moutan Cortex is a valuable medicinal resource rich in bioactive polyphenols, yet its sustainable valorization is often hindered by the inefficiencies and environmental concerns associated with conventional extraction methods. In this study, a green and efficient ultrasound-assisted extraction (UAE) strategy was developed using a betaine-levulinic acid (Bet-LevA) natural deep eutectic solvent (NADES). An intelligent optimization approach was applied to determine the optimal extraction conditions, achieving a maximum total polyphenol content of 27.03 ± 0.41 mg GAE/g DW, which was 1.93-fold higher than that obtained using 70% ethanol extraction. The molecular mechanisms underlying the superior extraction performance of Bet-LevA were further elucidated using FT-IR, conductor-like screening model for realistic solvents (COSMO-RS), and molecular dynamics (MD) simulations, revealing that hydrogen-bond networks and stronger solvent-polyphenol interactions promoted solubilization and stabilization of phenolic compounds. Furthermore, the extracted polyphenol fraction effectively alleviated fatty acid-induced lipid accumulation in AML-12 hepatocytes. Overall, this study provides a sustainable extraction framework integrating green solvent design, intelligent optimization, molecular mechanism analysis, and functional evaluation for the high-value utilization of Moutan Cortex in functional foods and nutraceutical applications.<br /> (Copyright © 2026 The Authors. Published by Elsevier B.V. All rights reserved.)
– Name: Abstract
  Label: Competing Interests
  Group: Ab
  Data: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
– Name: SubjectMinor
  Label: Contributed Indexing
  Group:
  Data: <i>Keywords: </i>COSMO-RS; Lipid-lowering activity; Molecular dynamics simulation; MoutanCortex; Natural deep eutectic solvents; Polyphenols; Ultrasound-assisted extraction
– Name: NumberCAS
  Label: Substance Nomenclature
  Group: ID
  Data: 0 (Polyphenols)<br />0 (moutan cortex)<br />0 (Deep Eutectic Solvents)<br />0 (Drugs, Chinese Herbal)<br />0 (Solvents)
– Name: DateEntry
  Label: Entry Date(s)
  Group: Date
  Data: <i>Date Created: </i>20260912 <i>Date Completed: </i>20261005 <i>Latest Revision: </i>20261005
– Name: DateUpdate
  Label: Update Code
  Group: Date
  Data: 20261006
– Name: PubmedCentralID
  Label: PubMed Central ID
  Group: ID
  Data: PMC13587915
– Name: DOI
  Label: DOI
  Group: ID
  Data: 10.1016/j.ultsonch.2026.108052
– Name: AN
  Label: PMID
  Group: ID
  Data: 42731572
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=cmedm&AN=42731572
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1016/j.ultsonch.2026.108052
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        StartPage: 108052
    Subjects:
      – SubjectFull: Solvents chemistry
        Type: general
      – SubjectFull: Molecular Dynamics Simulation
        Type: general
      – SubjectFull: Animals
        Type: general
      – SubjectFull: Polyphenols isolation & purification
        Type: general
      – SubjectFull: Polyphenols pharmacology
        Type: general
      – SubjectFull: Polyphenols chemistry
        Type: general
      – SubjectFull: Paeonia chemistry
        Type: general
      – SubjectFull: Chemical Fractionation methods
        Type: general
      – SubjectFull: Deep Eutectic Solvents chemistry
        Type: general
      – SubjectFull: Drugs, Chinese Herbal chemistry
        Type: general
      – SubjectFull: Ultrasonic Waves
        Type: general
    Titles:
      – TitleFull: Ultrasound-assisted extraction of polyphenols from Moutan Cortex using natural deep eutectic solvent: Optimization, molecular mechanisms, and lipid-lowering activity.
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Yin S
      – PersonEntity:
          Name:
            NameFull: Peng W
      – PersonEntity:
          Name:
            NameFull: Wang Y
      – PersonEntity:
          Name:
            NameFull: Lu H
      – PersonEntity:
          Name:
            NameFull: Liu P
      – PersonEntity:
          Name:
            NameFull: Zhu X
      – PersonEntity:
          Name:
            NameFull: Yang H
      – PersonEntity:
          Name:
            NameFull: Li J
      – PersonEntity:
          Name:
            NameFull: Li L
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 01
              M: 10
              Text: 2026 Oct
              Type: published
              Y: 2026
          Identifiers:
            – Type: issn-electronic
              Value: 1873-2828
          Numbering:
            – Type: volume
              Value: 133
          Titles:
            – TitleFull: Ultrasonics sonochemistry
              Type: main
ResultId 1