Academic Journal
MAFF is a transcription factor that regulates contraction-induced skeletal muscle transcription.
| Τίτλος: | MAFF is a transcription factor that regulates contraction-induced skeletal muscle transcription. |
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| Συγγραφείς: | Fukushima T; Department of Physiology, School of Medicine, Aichi Medical University, Nagakute, Japan.; Laboratory of Stem Cell Regeneration and Adaptation, Graduate School of Pharmaceutical Sciences, The University of Osaka, Suita, Japan., Fujioka T; Department of Physiology, School of Medicine, Aichi Medical University, Nagakute, Japan., Masubuchi S; Department of Physiology, School of Medicine, Aichi Medical University, Nagakute, Japan., Sakakibara I; Department of Physiology, School of Medicine, Aichi Medical University, Nagakute, Japan sakakibara.iori.711@mail.aichi-med-u.ac.jp. |
| Πηγή: | Life science alliance [Life Sci Alliance] 2026 Sep 08; Vol. 9 (12). Date of Electronic Publication: 2026 Sep 08 (Print Publication: 2026). |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Life Science Alliance, LLC Country of Publication: United States NLM ID: 101728869 Publication Model: Electronic-Print Cited Medium: Internet ISSN: 2575-1077 (Electronic) Linking ISSN: 25751077 NLM ISO Abbreviation: Life Sci Alliance Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: [Woodbury, NY] : Life Science Alliance, LLC, [2018]- |
| Ιατρικοί όροι (MeSH): | Muscle, Skeletal*/metabolism , Muscle, Skeletal*/physiology , Muscle Contraction*/genetics , Muscle Contraction*/physiology , Transcription Factors*/metabolism , Transcription Factors*/genetics, Muscle Development/genetics ; Muscle Fibers, Skeletal/metabolism ; Animals ; Mice ; Humans ; Gene Expression Regulation ; Transcription, Genetic ; Cell Line ; Electric Stimulation |
| Περίληψη: | Skeletal muscle adapts to exercise through rapid transcriptional remodeling, but regulators that link contractile activity to these gene programs remain unclear. Here we show that MAFF, a small MAF transcription factor, is consistently induced by acute exercise in both human and mouse skeletal muscle, with induction restricted to muscles recruited by the exercise modality. In C2C12 cells, Maff was induced by in vitro electrical pulse stimulation model. Using CRISPR/Cas9-mediated Maff knockout C2C12 cells, we found that loss of Maff reduced myogenic fusion with accumulation of unfused nuclei. RNA sequencing revealed broad reprogramming in Maff-deficient myotubes, including reduced expression of muscle structural and contractile genes and increased interferon/immune responses and extracellular matrix-related signatures. Moreover, Maff deficiency attenuated a subset of electrical pulse stimulation-responsive genes. Together, these data identify MAFF as a component of the transcriptional machinery supporting myogenic maturation and contraction-evoked gene regulation. (© 2026 Fukushima et al.) |
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| Substance Nomenclature: | 0 (Transcription Factors) |
| Entry Date(s): | Date Created: 20260908 Date Completed: 20260908 Latest Revision: 20260911 |
| Update Code: | 20260911 |
| PubMed Central ID: | PMC13554480 |
| DOI: | 10.26508/lsa.202603709 |
| PMID: | 42711121 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 2575-1077 |
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| DOI: | 10.26508/lsa.202603709 |