Academic Journal
Hydrogen peroxide contributes to SYP-14288-induced multidrug resistance in Rhizoctonia solani by modulating detoxification and efflux pathways.
| Τίτλος: | Hydrogen peroxide contributes to SYP-14288-induced multidrug resistance in Rhizoctonia solani by modulating detoxification and efflux pathways. |
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| Συγγραφείς: | Liang Z; Department of Plant Pathology, China Agricultural University, Beijing 100193, China., Zhang J; Department of Plant Pathology, China Agricultural University, Beijing 100193, China., Zhou R; Department of Plant Pathology, China Agricultural University, Beijing 100193, China., Wu Z; Department of Plant Pathology, China Agricultural University, Beijing 100193, China; College of Forestry, Northwest A & F University, Yangling 712100, Shaanxi, China., Deng W; Department of Plant Pathology, China Agricultural University, Beijing 100193, China., Zhu L; Department of Plant Pathology, China Agricultural University, Beijing 100193, China., Hu M; Shenzhen Inspection and Testing Center of Agricultural Product Quality and Safety, Shenzheng 518000, Guangzhou, China., Yue X; Shenzhen Inspection and Testing Center of Agricultural Product Quality and Safety, Shenzheng 518000, Guangzhou, China., Hao J; School of Food and Agriculture, University of Maine, Orono, ME 04469, USA., Liu P; Department of Plant Pathology, China Agricultural University, Beijing 100193, China. Electronic address: pengfeiliu@cau.edu.cn., Liu X; Department of Plant Pathology, China Agricultural University, Beijing 100193, China. |
| Πηγή: | Pesticide biochemistry and physiology [Pestic Biochem Physiol] 2026 Sep; Vol. 223, pp. 107296. Date of Electronic Publication: 2026 Aug 03. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Academic Press Country of Publication: United States NLM ID: 1301573 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1095-9939 (Electronic) Linking ISSN: 00483575 NLM ISO Abbreviation: Pestic Biochem Physiol Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: New York Ny : Academic Press |
| Ιατρικοί όροι (MeSH): | Rhizoctonia*/drug effects , Rhizoctonia*/metabolism , Rhizoctonia*/genetics , Hydrogen Peroxide*/metabolism , Hydrogen Peroxide*/pharmacology , Fungicides, Industrial*/pharmacology , Drug Resistance, Multiple, Fungal*/genetics, Oxidative Stress/drug effects ; Drug Resistance, Fungal |
| Περίληψη: | SYP-14288 is an effective antifungal compound against Rhizoctonia solani, the causal agent of rice sheath blight. The chemical acts through its unique uncoupling mechanism. Previous studies have demonstrated that SYP-14288-resistant mutants of R. solani develop multidrug resistance (MDR), but the underlying mechanisms remain unclear. In this study, in SYP-14288-resistant mutants, hydrogen peroxide (H₂O₂) accumulated at higher levels, which may activated oxidative stress signaling and upregulated detoxification pathways. Compared with the parental strain, these mutants also showed significantly reduced sensitivity to exogenous H₂O₂. Transcriptomic and biochemical analyses revealed increased expression of cytochrome P450 monooxygenases, glutathione S-transferases, and efflux transporters, all contributing to the MDR phenotype. Reducing H₂O₂ levels suppressed detoxification activity and partially restored sensitivity to SYP-14288, confirming a putatively causal role of oxidative signaling. Moreover, both H₂O₂ and SYP-14288 synergistically enhanced the expression of genes associated with efflux-, metabolism-, and signaling-related genes in resistant mutants. Exogenous H₂O₂ increased the metabolism of SYP-14288, whereas N-acetylcysteine (an antioxidant) reduced it. Our findings demonstrate that SYP-14288-induced intracellular H₂O₂ accumulation putatively contributes the upregulation of metabolism- and efflux-related genes, thereby enhancing detoxification and contributing to MDR. This study provides novel mechanistic insights into the role of H₂O₂ in mediating R. solani resistance to SYP-14288, with important implications for the management of fungicide resistance. (Copyright © 2026. Published by Elsevier Inc.) |
| Competing Interests: | Declaration of competing interest None. |
| Contributed Indexing: | Keywords: ABC transporters; Cytochrome P450; Detoxification metabolism; Fungicide resistance; Reactive oxygen species (ROS); Uncoupler |
| Substance Nomenclature: | BBX060AN9V (Hydrogen Peroxide) 0 (Fungicides, Industrial) |
| SCR Organism: | Rhizoctonia solani |
| Entry Date(s): | Date Created: 20260904 Date Completed: 20260904 Latest Revision: 20260904 |
| Update Code: | 20260905 |
| DOI: | 10.1016/j.pestbp.2026.107296 |
| PMID: | 42697665 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1095-9939 |
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| DOI: | 10.1016/j.pestbp.2026.107296 |