Academic Journal
Lycopene Alleviates Fatty Acid-Induced Oxidative Stress and Apoptosis in Bovine Mammary Epithelial Cells via the TFEB-Mediated Autophagy Signaling Pathway.
| Τίτλος: | Lycopene Alleviates Fatty Acid-Induced Oxidative Stress and Apoptosis in Bovine Mammary Epithelial Cells via the TFEB-Mediated Autophagy Signaling Pathway. |
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| Συγγραφείς: | Sun X; Heilongjiang Provincial Key Laboratory of Prevention and Control of Bovine Diseases, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, No. 5 Xinfeng Road, Daqing, Heilongjiang Province163319, China., Jia H; Heilongjiang Provincial Key Laboratory of Prevention and Control of Bovine Diseases, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, No. 5 Xinfeng Road, Daqing, Heilongjiang Province163319, China., Chen Y; Heilongjiang Provincial Key Laboratory of Prevention and Control of Bovine Diseases, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, No. 5 Xinfeng Road, Daqing, Heilongjiang Province163319, China., Wu Y; Heilongjiang Provincial Key Laboratory of Prevention and Control of Bovine Diseases, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, No. 5 Xinfeng Road, Daqing, Heilongjiang Province163319, China., Yan J; Heilongjiang Provincial Key Laboratory of Prevention and Control of Bovine Diseases, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, No. 5 Xinfeng Road, Daqing, Heilongjiang Province163319, China., Yu Y; Heilongjiang Provincial Key Laboratory of Prevention and Control of Bovine Diseases, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, No. 5 Xinfeng Road, Daqing, Heilongjiang Province163319, China., Wang J; Heilongjiang Provincial Key Laboratory of Prevention and Control of Bovine Diseases, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, No. 5 Xinfeng Road, Daqing, Heilongjiang Province163319, China., Ding W; Heilongjiang Provincial Key Laboratory of Prevention and Control of Bovine Diseases, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, No. 5 Xinfeng Road, Daqing, Heilongjiang Province163319, China., Ma L; Heilongjiang Provincial Key Laboratory of Prevention and Control of Bovine Diseases, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, No. 5 Xinfeng Road, Daqing, Heilongjiang Province163319, China., Xu Q; Heilongjiang Provincial Key Laboratory of Prevention and Control of Bovine Diseases, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, No. 5 Xinfeng Road, Daqing, Heilongjiang Province163319, China., Loor JJ; Mammalian NutriPhysioGenomics, Department of Animal Sciences and Division of Nutritional Sciences, University of Illinois, Urbana, Illinois61801, United States., Xu C; College of Veterinary Medicine, China Agricultural University, Beijing100193, China., Sun X; Heilongjiang Provincial Key Laboratory of Prevention and Control of Bovine Diseases, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, No. 5 Xinfeng Road, Daqing, Heilongjiang Province163319, China. |
| Πηγή: | Journal of agricultural and food chemistry [J Agric Food Chem] 2026 Sep 02; Vol. 74 (34), pp. 27111-27122. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: American Chemical Society Country of Publication: United States NLM ID: 0374755 Publication Model: Print Cited Medium: Internet ISSN: 1520-5118 (Electronic) Linking ISSN: 00218561 NLM ISO Abbreviation: J Agric Food Chem Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Washington, American Chemical Society. |
| Ιατρικοί όροι (MeSH): | Autophagy*/drug effects , Oxidative Stress*/drug effects , Epithelial Cells*/metabolism , Epithelial Cells*/drug effects , Epithelial Cells*/cytology , Apoptosis*/drug effects , Lycopene*/pharmacology , Mammary Glands, Animal*/cytology , Mammary Glands, Animal*/metabolism , Mammary Glands, Animal*/drug effects , Basic Helix-Loop-Helix Leucine Zipper Transcription Factors*/metabolism , Basic Helix-Loop-Helix Leucine Zipper Transcription Factors*/genetics , Fatty Acids*/metabolism, Cattle/metabolism ; Signal Transduction/drug effects ; Reactive Oxygen Species/metabolism ; Animals ; Female ; Cell Line |
| Περίληψη: | High concentrations of free fatty acids (FFA) during early lactation cause metabolic stress and oxidative damage in bovine mammary epithelial cells. This study explored the protective mechanism of lycopene (LYC), a natural antioxidant, against FFA-induced injury in the MAC-T cells. We found that 1.2 mM FFA induced oxidative stress, reactive oxygen species (ROS) accumulation, apoptosis, and impaired autophagy. LYC dose-dependently alleviated FFA-induced oxidative damage, restored autophagy flux, upregulated ATG5 autophagy-related 5 (ATG5), LC3-II, and transcription factor EB (TFEB), and downregulated p62, ROS, and apoptosis. Inhibition of autophagy abolished the protective effects of the LYC. Overexpression of TFEB mitigated FFA-induced dysfunction, while TFEB knockdown aggravated cell injury. These results demonstrate that LYC protects mammary epithelial cells from FFA-induced oxidative damage via regulating TFEB-mediated autophagy, suggesting its potential use in alleviating metabolic stress in periparturient dairy cows. (© 2026 American Chemical Society.) |
| Grant Information: | YJSCX2025-KJQN46 Postgraduate Innovation Research Project of Heilongjiang Bayi Agricultural University; YJSCX2025-Z07 Postgraduate Innovation Research Project of Heilongjiang Bayi Agricultural University |
| Contributed Indexing: | Keywords: apoptosis; autophagy; bovine mammary epithelial cells; lycopene; transcription factor EB |
| Substance Nomenclature: | SB0N2N0WV6 (Lycopene) 0 (Reactive Oxygen Species) 0 (Basic Helix-Loop-Helix Leucine Zipper Transcription Factors) 0 (Fatty Acids) |
| Entry Date(s): | Date Created: 20260903 Date Completed: 20260903 Latest Revision: 20260903 |
| Update Code: | 20260904 |
| DOI: | 10.1021/acs.jafc.6c05011 |
| PMID: | 42690786 |
| Βάση Δεδομένων: | MEDLINE |
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